Trypsin Digestion Pretreatment of Kelp Samples and the Iodine Speciation Analysis of Iodate, Iodide, MIT, and DIT by HPLC-ICP-MS
The analysis of iodine content in kelp is mainly focused on determining total iodine content, which lacks a rapid and sensitive analytical method for iodine species corresponding to iodate, iodide, monoiodotyrosine (MIT), and diiodo-tyrosine (DIT). Based on exploring the pretreatment methods of enzy...
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Wiley
2023-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2023/5072070 |
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| author | Tong Chen Wanjing Cui Ziwei Bi Yafei Guo Guoliang Zhu Xiuyun Pan Zhiqiang Cui Zhen Yang Tianlong Deng |
| author_facet | Tong Chen Wanjing Cui Ziwei Bi Yafei Guo Guoliang Zhu Xiuyun Pan Zhiqiang Cui Zhen Yang Tianlong Deng |
| author_sort | Tong Chen |
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| description | The analysis of iodine content in kelp is mainly focused on determining total iodine content, which lacks a rapid and sensitive analytical method for iodine species corresponding to iodate, iodide, monoiodotyrosine (MIT), and diiodo-tyrosine (DIT). Based on exploring the pretreatment methods of enzymatic hydrolysis of kelp, a rapid determination method of high-performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC-ICP-MS) for different iodine species existed in kelp samples was established. The pretreatment method is trypsin hydrolysis of kelp samples treated at 50°C for 10 hours, then the extraction solution was obtained by centrifugation, and filtration was proved for the first time. A high-efficiency separation anion-exchange column (Dionex IonPac AS-14) for iodate, iodide, MIT, and DIT with 0.1 mol·L−1(NH4)2CO3 solution at pH = 9 as a mobile phase, a flow rate of 0.8 mL·min−1 for the first 1500 s, and 1.2 mL·min−1 for the last 1800 s was confirmed. The linear ranges of the standard calibrations on four iodine species are 1.0–150 μg·L−1, and the detection limits are 1.62, 1.02, 1.59, and 2.55 μg·L−1, respectively, while the RSD values of four iodine species are all less than 2%. Under the optimized conditions, the proposed method was successfully applied for the speciation analysis of iodine in kelp samples. |
| format | Article |
| id | doaj-art-8f36c6d80c9b46c4bc74aec1e4c796ed |
| institution | DOAJ |
| issn | 2090-9071 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
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| series | Journal of Chemistry |
| spelling | doaj-art-8f36c6d80c9b46c4bc74aec1e4c796ed2025-08-20T03:19:46ZengWileyJournal of Chemistry2090-90712023-01-01202310.1155/2023/5072070Trypsin Digestion Pretreatment of Kelp Samples and the Iodine Speciation Analysis of Iodate, Iodide, MIT, and DIT by HPLC-ICP-MSTong Chen0Wanjing Cui1Ziwei Bi2Yafei Guo3Guoliang Zhu4Xiuyun Pan5Zhiqiang Cui6Zhen Yang7Tianlong Deng8Key Laboratory of Marine Resource Chemistry and Food TechnologyKey Laboratory of Marine Resource Chemistry and Food TechnologyKey Laboratory of Marine Resource Chemistry and Food TechnologyKey Laboratory of Marine Resource Chemistry and Food TechnologyChina Salt Engineering Technology Research Institute Co.Hubei Yixin Salt Industry Technology Research Institute Co.China Salt Engineering Technology Research Institute Co.China Salt Engineering Technology Research Institute Co.Key Laboratory of Marine Resource Chemistry and Food TechnologyThe analysis of iodine content in kelp is mainly focused on determining total iodine content, which lacks a rapid and sensitive analytical method for iodine species corresponding to iodate, iodide, monoiodotyrosine (MIT), and diiodo-tyrosine (DIT). Based on exploring the pretreatment methods of enzymatic hydrolysis of kelp, a rapid determination method of high-performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC-ICP-MS) for different iodine species existed in kelp samples was established. The pretreatment method is trypsin hydrolysis of kelp samples treated at 50°C for 10 hours, then the extraction solution was obtained by centrifugation, and filtration was proved for the first time. A high-efficiency separation anion-exchange column (Dionex IonPac AS-14) for iodate, iodide, MIT, and DIT with 0.1 mol·L−1(NH4)2CO3 solution at pH = 9 as a mobile phase, a flow rate of 0.8 mL·min−1 for the first 1500 s, and 1.2 mL·min−1 for the last 1800 s was confirmed. The linear ranges of the standard calibrations on four iodine species are 1.0–150 μg·L−1, and the detection limits are 1.62, 1.02, 1.59, and 2.55 μg·L−1, respectively, while the RSD values of four iodine species are all less than 2%. Under the optimized conditions, the proposed method was successfully applied for the speciation analysis of iodine in kelp samples.http://dx.doi.org/10.1155/2023/5072070 |
| spellingShingle | Tong Chen Wanjing Cui Ziwei Bi Yafei Guo Guoliang Zhu Xiuyun Pan Zhiqiang Cui Zhen Yang Tianlong Deng Trypsin Digestion Pretreatment of Kelp Samples and the Iodine Speciation Analysis of Iodate, Iodide, MIT, and DIT by HPLC-ICP-MS Journal of Chemistry |
| title | Trypsin Digestion Pretreatment of Kelp Samples and the Iodine Speciation Analysis of Iodate, Iodide, MIT, and DIT by HPLC-ICP-MS |
| title_full | Trypsin Digestion Pretreatment of Kelp Samples and the Iodine Speciation Analysis of Iodate, Iodide, MIT, and DIT by HPLC-ICP-MS |
| title_fullStr | Trypsin Digestion Pretreatment of Kelp Samples and the Iodine Speciation Analysis of Iodate, Iodide, MIT, and DIT by HPLC-ICP-MS |
| title_full_unstemmed | Trypsin Digestion Pretreatment of Kelp Samples and the Iodine Speciation Analysis of Iodate, Iodide, MIT, and DIT by HPLC-ICP-MS |
| title_short | Trypsin Digestion Pretreatment of Kelp Samples and the Iodine Speciation Analysis of Iodate, Iodide, MIT, and DIT by HPLC-ICP-MS |
| title_sort | trypsin digestion pretreatment of kelp samples and the iodine speciation analysis of iodate iodide mit and dit by hplc icp ms |
| url | http://dx.doi.org/10.1155/2023/5072070 |
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