Development of functional markers for peroxidase activity genes TaPod-7AS and TaPod-4AL in wheat
Abstract Grain peroxidase (POD) has a pivotal role in determining wheat flour color and end-use products. High POD activity in wheat grains can improve the whiteness and commercial value of flour. The POD genes TaPod-7AS and TaPod-4AL have high effects on POD activity in wheat. Function marker for P...
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2025-05-01
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| Online Access: | https://doi.org/10.1186/s12864-025-11597-8 |
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| author | Xinyuan Liu Yukun Cheng Hongwei Geng Yi Ren Junmei Cao Bin Bai Yating Xue |
| author_facet | Xinyuan Liu Yukun Cheng Hongwei Geng Yi Ren Junmei Cao Bin Bai Yating Xue |
| author_sort | Xinyuan Liu |
| collection | DOAJ |
| description | Abstract Grain peroxidase (POD) has a pivotal role in determining wheat flour color and end-use products. High POD activity in wheat grains can improve the whiteness and commercial value of flour. The POD genes TaPod-7AS and TaPod-4AL have high effects on POD activity in wheat. Function marker for POD genes can be used to improve flour color attributes accurately and efficiently in wheat molecular breeding. Here, wheat POD genes TaPod-7AS and TaPod-4AL were sequenced and characterized, gene expression analysis indicated that TaPod-7AS, TaPod-4AL, TaPod-D1 genes were highly expressed at 21 days after flowering. Function markers POD-7AS1 and POD-7AS2 were developed for TaPod-7AS, amplifying 424-bp and 464-bp PCR fragments in the cultivars with low and high POD activities, respectively. The markers POD-4AL1 and POD-4AL2 for TaPod-4AL amplified 541-bp and 391-bp fragments in the cultivars with low and high POD activities, respectively. The functional markers were validated in a diverse panel of 268 winter wheat cultivars and advanced lines, indicating significant associations between genotypes and POD activity (P < 0.05). The genotypes with favorable alleles TaPod-7ASb/TaPod-4ALb/TaPod-D1b had higher POD activity (749.9 U.g-1.min-1) than those with alleles TaPod-7ASa/TaPod-4ALa/TaPod-D1a (mean POD activity 619.4 U.g-1.min-1). |
| format | Article |
| id | doaj-art-8f2516be5ea947a5bf167bb2fea1f146 |
| institution | DOAJ |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
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| series | BMC Genomics |
| spelling | doaj-art-8f2516be5ea947a5bf167bb2fea1f1462025-08-20T03:16:31ZengBMCBMC Genomics1471-21642025-05-0126111510.1186/s12864-025-11597-8Development of functional markers for peroxidase activity genes TaPod-7AS and TaPod-4AL in wheatXinyuan Liu0Yukun Cheng1Hongwei Geng2Yi Ren3Junmei Cao4Bin Bai5Yating Xue6College of Agronomy, Xinjiang Agricultural UniversityCollege of Agronomy, Xinjiang Agricultural UniversityCollege of Agronomy, Xinjiang Agricultural UniversityCollege of Agronomy, Xinjiang Agricultural UniversityResearch Institute of Food Crops, Xinjiang Academy of Agricultural SciencesWheat Research Institute, Gansu Academy of Agricultural SciencesCollege of Agronomy, Xinjiang Agricultural UniversityAbstract Grain peroxidase (POD) has a pivotal role in determining wheat flour color and end-use products. High POD activity in wheat grains can improve the whiteness and commercial value of flour. The POD genes TaPod-7AS and TaPod-4AL have high effects on POD activity in wheat. Function marker for POD genes can be used to improve flour color attributes accurately and efficiently in wheat molecular breeding. Here, wheat POD genes TaPod-7AS and TaPod-4AL were sequenced and characterized, gene expression analysis indicated that TaPod-7AS, TaPod-4AL, TaPod-D1 genes were highly expressed at 21 days after flowering. Function markers POD-7AS1 and POD-7AS2 were developed for TaPod-7AS, amplifying 424-bp and 464-bp PCR fragments in the cultivars with low and high POD activities, respectively. The markers POD-4AL1 and POD-4AL2 for TaPod-4AL amplified 541-bp and 391-bp fragments in the cultivars with low and high POD activities, respectively. The functional markers were validated in a diverse panel of 268 winter wheat cultivars and advanced lines, indicating significant associations between genotypes and POD activity (P < 0.05). The genotypes with favorable alleles TaPod-7ASb/TaPod-4ALb/TaPod-D1b had higher POD activity (749.9 U.g-1.min-1) than those with alleles TaPod-7ASa/TaPod-4ALa/TaPod-D1a (mean POD activity 619.4 U.g-1.min-1).https://doi.org/10.1186/s12864-025-11597-8Functional markerGrain POD geneTaPod-7ASTaPod-4ALTriticum aestivum |
| spellingShingle | Xinyuan Liu Yukun Cheng Hongwei Geng Yi Ren Junmei Cao Bin Bai Yating Xue Development of functional markers for peroxidase activity genes TaPod-7AS and TaPod-4AL in wheat BMC Genomics Functional marker Grain POD gene TaPod-7AS TaPod-4AL Triticum aestivum |
| title | Development of functional markers for peroxidase activity genes TaPod-7AS and TaPod-4AL in wheat |
| title_full | Development of functional markers for peroxidase activity genes TaPod-7AS and TaPod-4AL in wheat |
| title_fullStr | Development of functional markers for peroxidase activity genes TaPod-7AS and TaPod-4AL in wheat |
| title_full_unstemmed | Development of functional markers for peroxidase activity genes TaPod-7AS and TaPod-4AL in wheat |
| title_short | Development of functional markers for peroxidase activity genes TaPod-7AS and TaPod-4AL in wheat |
| title_sort | development of functional markers for peroxidase activity genes tapod 7as and tapod 4al in wheat |
| topic | Functional marker Grain POD gene TaPod-7AS TaPod-4AL Triticum aestivum |
| url | https://doi.org/10.1186/s12864-025-11597-8 |
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