Comparative Analysis of Rhizosphere Microbiomes in Different Blueberry Cultivars
Blueberry growth is closely tied to its rhizosphere’s microbial communities. Recent advancements in high-throughput sequencing and multi-omics technologies have enhanced the investigation of variations in rhizosphere microbial communities and their functional roles across different plant cultivars....
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MDPI AG
2025-06-01
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| author | Lifeng Xiao Qiuyue Zhao Jie Deng Lingyan Cui Tingting Zhang Qin Yang Sifeng Zhao |
| author_facet | Lifeng Xiao Qiuyue Zhao Jie Deng Lingyan Cui Tingting Zhang Qin Yang Sifeng Zhao |
| author_sort | Lifeng Xiao |
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| description | Blueberry growth is closely tied to its rhizosphere’s microbial communities. Recent advancements in high-throughput sequencing and multi-omics technologies have enhanced the investigation of variations in rhizosphere microbial communities and their functional roles across different plant cultivars. In this study, high-throughput sequencing was utilized to assess the rhizosphere microbial diversity in highbush and rabbiteye blueberry groups, encompassing a total of eight cultivars. Notable variations were observed in both bacterial and fungal community diversity. Ten bacterial phyla, each with a relative abundance greater than 1%, constituted 92.32–97.08% of the total abundance across the eight cultivars, with <i>Acidobacteriota</i>, <i>Actinobacteriota</i>, and <i>Pseudomonadota</i> being predominant. Similarly, five major fungal phyla, each exceeding 1% in relative abundance, accounted for 88.18–97.20% of the total abundance, with <i>Ascomycota</i> and <i>Basidiomycota</i> being the most dominant. The results showed that the rhizospheres of blueberries host a variety of plant growth-promoting rhizobacteria (PGPR), including genera such as <i>Burkholderia</i>, <i>Enterobacter</i>, <i>Streptomyces</i>, <i>Arthrobacter</i>, and <i>Pseudomonas</i>. Rabbiteye blueberry cultivars exhibit a greater propensity for accumulating beneficial symbiotic microorganisms compared to highbush cultivars. Notably, the relative abundance of ericoid mycorrhizal fungi, specifically <i>Oidiodendron</i>, is significantly elevated in the cultivars Emerald, Premier, O’Neal, and Brightwell, with the most pronounced increase observed in Emerald. Furthermore, rabbiteye blueberries support a more diverse and abundant array of cultivar-specific fungal communities than their highbush counterparts. Understanding the interaction networks between blueberries and their associated microbes can provide a theoretical foundation for the targeted regulation of rhizosphere microbiomes and offer valuable insights for the management of rhizospheres in other acidophilic crops. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-06-01 |
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| series | Horticulturae |
| spelling | doaj-art-8f0f44ec0a9b421280ce5bc2493bda4e2025-08-20T03:27:14ZengMDPI AGHorticulturae2311-75242025-06-0111669610.3390/horticulturae11060696Comparative Analysis of Rhizosphere Microbiomes in Different Blueberry CultivarsLifeng Xiao0Qiuyue Zhao1Jie Deng2Lingyan Cui3Tingting Zhang4Qin Yang5Sifeng Zhao6Provincial Famous Teacher Yang Qin Studio, Key Laboratory of Molecular Breeding and Variety Creation of Horticultural Plants for Mountain Features in Guizhou Province, School of Life and Health Science, Kaili University, Kaili 556011, ChinaKey Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization, Shihezi University, Shihezi 832003, ChinaProvincial Famous Teacher Yang Qin Studio, Key Laboratory of Molecular Breeding and Variety Creation of Horticultural Plants for Mountain Features in Guizhou Province, School of Life and Health Science, Kaili University, Kaili 556011, ChinaProvincial Famous Teacher Yang Qin Studio, Key Laboratory of Molecular Breeding and Variety Creation of Horticultural Plants for Mountain Features in Guizhou Province, School of Life and Health Science, Kaili University, Kaili 556011, ChinaProvincial Famous Teacher Yang Qin Studio, Key Laboratory of Molecular Breeding and Variety Creation of Horticultural Plants for Mountain Features in Guizhou Province, School of Life and Health Science, Kaili University, Kaili 556011, ChinaProvincial Famous Teacher Yang Qin Studio, Key Laboratory of Molecular Breeding and Variety Creation of Horticultural Plants for Mountain Features in Guizhou Province, School of Life and Health Science, Kaili University, Kaili 556011, ChinaKey Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization, Shihezi University, Shihezi 832003, ChinaBlueberry growth is closely tied to its rhizosphere’s microbial communities. Recent advancements in high-throughput sequencing and multi-omics technologies have enhanced the investigation of variations in rhizosphere microbial communities and their functional roles across different plant cultivars. In this study, high-throughput sequencing was utilized to assess the rhizosphere microbial diversity in highbush and rabbiteye blueberry groups, encompassing a total of eight cultivars. Notable variations were observed in both bacterial and fungal community diversity. Ten bacterial phyla, each with a relative abundance greater than 1%, constituted 92.32–97.08% of the total abundance across the eight cultivars, with <i>Acidobacteriota</i>, <i>Actinobacteriota</i>, and <i>Pseudomonadota</i> being predominant. Similarly, five major fungal phyla, each exceeding 1% in relative abundance, accounted for 88.18–97.20% of the total abundance, with <i>Ascomycota</i> and <i>Basidiomycota</i> being the most dominant. The results showed that the rhizospheres of blueberries host a variety of plant growth-promoting rhizobacteria (PGPR), including genera such as <i>Burkholderia</i>, <i>Enterobacter</i>, <i>Streptomyces</i>, <i>Arthrobacter</i>, and <i>Pseudomonas</i>. Rabbiteye blueberry cultivars exhibit a greater propensity for accumulating beneficial symbiotic microorganisms compared to highbush cultivars. Notably, the relative abundance of ericoid mycorrhizal fungi, specifically <i>Oidiodendron</i>, is significantly elevated in the cultivars Emerald, Premier, O’Neal, and Brightwell, with the most pronounced increase observed in Emerald. Furthermore, rabbiteye blueberries support a more diverse and abundant array of cultivar-specific fungal communities than their highbush counterparts. Understanding the interaction networks between blueberries and their associated microbes can provide a theoretical foundation for the targeted regulation of rhizosphere microbiomes and offer valuable insights for the management of rhizospheres in other acidophilic crops.https://www.mdpi.com/2311-7524/11/6/696blueberrycultivar differences16S rRNAITSrhizosphere microbiome |
| spellingShingle | Lifeng Xiao Qiuyue Zhao Jie Deng Lingyan Cui Tingting Zhang Qin Yang Sifeng Zhao Comparative Analysis of Rhizosphere Microbiomes in Different Blueberry Cultivars Horticulturae blueberry cultivar differences 16S rRNA ITS rhizosphere microbiome |
| title | Comparative Analysis of Rhizosphere Microbiomes in Different Blueberry Cultivars |
| title_full | Comparative Analysis of Rhizosphere Microbiomes in Different Blueberry Cultivars |
| title_fullStr | Comparative Analysis of Rhizosphere Microbiomes in Different Blueberry Cultivars |
| title_full_unstemmed | Comparative Analysis of Rhizosphere Microbiomes in Different Blueberry Cultivars |
| title_short | Comparative Analysis of Rhizosphere Microbiomes in Different Blueberry Cultivars |
| title_sort | comparative analysis of rhizosphere microbiomes in different blueberry cultivars |
| topic | blueberry cultivar differences 16S rRNA ITS rhizosphere microbiome |
| url | https://www.mdpi.com/2311-7524/11/6/696 |
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