Anaesthetics reduce the viability of adipose-derived stem cells
Adipose-derived stem cells (ADSCs) are characterized by their low immunogenicity and unique immunosuppressive properties, providing many opportunities for autologous transplantation in regenerative medicine and plastic surgery. These methods are characterized by low rejection rates and intense stimu...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Adipocyte |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21623945.2024.2351870 |
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| author | Maria Bugajska-Liedtke Nadia Fatyga Aleksander Brzozowski Anna Bajek Małgorzata Maj |
| author_facet | Maria Bugajska-Liedtke Nadia Fatyga Aleksander Brzozowski Anna Bajek Małgorzata Maj |
| author_sort | Maria Bugajska-Liedtke |
| collection | DOAJ |
| description | Adipose-derived stem cells (ADSCs) are characterized by their low immunogenicity and unique immunosuppressive properties, providing many opportunities for autologous transplantation in regenerative medicine and plastic surgery. These methods are characterized by low rejection rates and intense stimulation of tissue regeneration. However, procedures during which fat tissue is harvested occur under local anaesthesia. To better understand the effects and mechanisms of anaesthetic compounds in cosmetic and therapeutic procedures, the present study used a mixture of these compounds (0.1% epinephrine, 8.4% sodium bicarbonate, and 4% articaine) and examined their impact on a human adipose-derived stem cell line. The results showed anesthetics’ negative, dose-dependent effect on cell viability and proliferation, especially during the first 24 h of incubation. After extending the exposure to 48 and 72 h of incubation, cells adapted to new culture conditions. In contrast, no significant changes were observed in immunophenotype, cell cycle progression, and apoptosis. The results obtained from this study provide information on the effect of the selected mixture of anaesthetics on the characteristics and function of ASC52telo cells. The undesirable changes in the metabolic activity of cells suggest the need to search for new drugs to harvest cells with unaltered properties and higher efficacy in aesthetic medicine treatments. |
| format | Article |
| id | doaj-art-8efcdd172e5f4c3c88dfab424a8bdd3c |
| institution | OA Journals |
| issn | 2162-3945 2162-397X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj-art-8efcdd172e5f4c3c88dfab424a8bdd3c2025-08-20T02:30:42ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2024-12-0113110.1080/21623945.2024.2351870Anaesthetics reduce the viability of adipose-derived stem cellsMaria Bugajska-Liedtke0Nadia Fatyga1Aleksander Brzozowski2Anna Bajek3Małgorzata Maj4Department of Tissue Engineering, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, PolandDepartment of Tissue Engineering, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, PolandDepartment of Tissue Engineering, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, PolandDepartment of Oncology, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, PolandDepartment of Tissue Engineering, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, PolandAdipose-derived stem cells (ADSCs) are characterized by their low immunogenicity and unique immunosuppressive properties, providing many opportunities for autologous transplantation in regenerative medicine and plastic surgery. These methods are characterized by low rejection rates and intense stimulation of tissue regeneration. However, procedures during which fat tissue is harvested occur under local anaesthesia. To better understand the effects and mechanisms of anaesthetic compounds in cosmetic and therapeutic procedures, the present study used a mixture of these compounds (0.1% epinephrine, 8.4% sodium bicarbonate, and 4% articaine) and examined their impact on a human adipose-derived stem cell line. The results showed anesthetics’ negative, dose-dependent effect on cell viability and proliferation, especially during the first 24 h of incubation. After extending the exposure to 48 and 72 h of incubation, cells adapted to new culture conditions. In contrast, no significant changes were observed in immunophenotype, cell cycle progression, and apoptosis. The results obtained from this study provide information on the effect of the selected mixture of anaesthetics on the characteristics and function of ASC52telo cells. The undesirable changes in the metabolic activity of cells suggest the need to search for new drugs to harvest cells with unaltered properties and higher efficacy in aesthetic medicine treatments.https://www.tandfonline.com/doi/10.1080/21623945.2024.2351870Adipose-derived stem cellsanaestheticscytotoxicitycell cycleapoptosis |
| spellingShingle | Maria Bugajska-Liedtke Nadia Fatyga Aleksander Brzozowski Anna Bajek Małgorzata Maj Anaesthetics reduce the viability of adipose-derived stem cells Adipocyte Adipose-derived stem cells anaesthetics cytotoxicity cell cycle apoptosis |
| title | Anaesthetics reduce the viability of adipose-derived stem cells |
| title_full | Anaesthetics reduce the viability of adipose-derived stem cells |
| title_fullStr | Anaesthetics reduce the viability of adipose-derived stem cells |
| title_full_unstemmed | Anaesthetics reduce the viability of adipose-derived stem cells |
| title_short | Anaesthetics reduce the viability of adipose-derived stem cells |
| title_sort | anaesthetics reduce the viability of adipose derived stem cells |
| topic | Adipose-derived stem cells anaesthetics cytotoxicity cell cycle apoptosis |
| url | https://www.tandfonline.com/doi/10.1080/21623945.2024.2351870 |
| work_keys_str_mv | AT mariabugajskaliedtke anaestheticsreducetheviabilityofadiposederivedstemcells AT nadiafatyga anaestheticsreducetheviabilityofadiposederivedstemcells AT aleksanderbrzozowski anaestheticsreducetheviabilityofadiposederivedstemcells AT annabajek anaestheticsreducetheviabilityofadiposederivedstemcells AT małgorzatamaj anaestheticsreducetheviabilityofadiposederivedstemcells |