Cloning and expression of Antheraea pernyi nucleopolyhedrovirus ptp-2 gene
To elucidate Antheraea pernyi nucleopolyhedrovirus (ApNPV) ptp-2 gene characters, ApNPV ptp-2 gene was cloned using primers based on the sequence of ApNPV PstI-C fragment and then sub-cloned into prokaryotic expression vector pQE-30 to construct recombinant plasmid pQE-ptp-2. PTP-2 protein was induc...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2007-03-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2007.02.0125 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849412783047704576 |
|---|---|
| author | SHI Sheng-lin PAN Min-hui LU Cheng |
| author_facet | SHI Sheng-lin PAN Min-hui LU Cheng |
| author_sort | SHI Sheng-lin |
| collection | DOAJ |
| description | To elucidate Antheraea pernyi nucleopolyhedrovirus (ApNPV) ptp-2 gene characters, ApNPV ptp-2 gene was cloned using primers based on the sequence of ApNPV PstI-C fragment and then sub-cloned into prokaryotic expression vector pQE-30 to construct recombinant plasmid pQE-ptp-2. PTP-2 protein was induced expression in E. coli M15. The expression accounted for 38.7 percent of the total protein. Western blot analysis of His-tag indicated that the protein expressed was His-fused. PTP-2 polyclonal antiserum was made against His-PTP-2 protein expressed in Escherichia coli. Western blot analysis of ApNPV occlusion body (OB) revealed that PTP-2 protein was not associated with the structure of ApNPV OB. |
| format | Article |
| id | doaj-art-8eef0590ae2447aeb3ace0b68f3d568f |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2007-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-8eef0590ae2447aeb3ace0b68f3d568f2025-08-20T03:34:20ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552007-03-013312512810.3785/1008-9209.2007.02.012510089209Cloning and expression of Antheraea pernyi nucleopolyhedrovirus ptp-2 geneSHI Sheng-linPAN Min-huiLU ChengTo elucidate Antheraea pernyi nucleopolyhedrovirus (ApNPV) ptp-2 gene characters, ApNPV ptp-2 gene was cloned using primers based on the sequence of ApNPV PstI-C fragment and then sub-cloned into prokaryotic expression vector pQE-30 to construct recombinant plasmid pQE-ptp-2. PTP-2 protein was induced expression in E. coli M15. The expression accounted for 38.7 percent of the total protein. Western blot analysis of His-tag indicated that the protein expressed was His-fused. PTP-2 polyclonal antiserum was made against His-PTP-2 protein expressed in Escherichia coli. Western blot analysis of ApNPV occlusion body (OB) revealed that PTP-2 protein was not associated with the structure of ApNPV OB.https://www.academax.com/doi/10.3785/1008-9209.2007.02.0125nucleopolyhedrovirusgene expressiongene clone<italic>Antheraea pernyi</italic><italic>ptp-2</italic> gene |
| spellingShingle | SHI Sheng-lin PAN Min-hui LU Cheng Cloning and expression of Antheraea pernyi nucleopolyhedrovirus ptp-2 gene 浙江大学学报. 农业与生命科学版 nucleopolyhedrovirus gene expression gene clone <italic>Antheraea pernyi</italic> <italic>ptp-2</italic> gene |
| title | Cloning and expression of Antheraea pernyi nucleopolyhedrovirus ptp-2 gene |
| title_full | Cloning and expression of Antheraea pernyi nucleopolyhedrovirus ptp-2 gene |
| title_fullStr | Cloning and expression of Antheraea pernyi nucleopolyhedrovirus ptp-2 gene |
| title_full_unstemmed | Cloning and expression of Antheraea pernyi nucleopolyhedrovirus ptp-2 gene |
| title_short | Cloning and expression of Antheraea pernyi nucleopolyhedrovirus ptp-2 gene |
| title_sort | cloning and expression of antheraea pernyi nucleopolyhedrovirus ptp 2 gene |
| topic | nucleopolyhedrovirus gene expression gene clone <italic>Antheraea pernyi</italic> <italic>ptp-2</italic> gene |
| url | https://www.academax.com/doi/10.3785/1008-9209.2007.02.0125 |
| work_keys_str_mv | AT shishenglin cloningandexpressionofantheraeapernyinucleopolyhedrovirusptp2gene AT panminhui cloningandexpressionofantheraeapernyinucleopolyhedrovirusptp2gene AT lucheng cloningandexpressionofantheraeapernyinucleopolyhedrovirusptp2gene |