Development of Double Haploid Lines from <i>Cucumber mosaic virus</i> Resistant Pepper Germplasm by Anther Culture
<i>Cucumber mosaic virus</i> (CMV) is one of the most dangerous viral diseases threatening <i>Solanaceae</i> crops, in particular <i>Capsicum</i> sp. This study aims to develop double haploid (DH) pepper lines from germplasm resistant to CMV in order to speed up t...
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2025-03-01
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| author | Zhana Ivanova Gancho Pasev Veronica Pashkoulova Vesela Radeva Stanislava Grozeva |
| author_facet | Zhana Ivanova Gancho Pasev Veronica Pashkoulova Vesela Radeva Stanislava Grozeva |
| author_sort | Zhana Ivanova |
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| description | <i>Cucumber mosaic virus</i> (CMV) is one of the most dangerous viral diseases threatening <i>Solanaceae</i> crops, in particular <i>Capsicum</i> sp. This study aims to develop double haploid (DH) pepper lines from germplasm resistant to CMV in order to speed up the breeding process. For this purpose, six genotypes previously tested for CMV resistance were used. Two induction mediums (17-2 and 17-3) with different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) for anther incubation and further plant regeneration were applied. L10 was the most responsive genotype, exhibiting the highest direct embryogenesis and the most plant regenerants on both mediums. Medium-specific response was observed in genotype L9 where regenerants were observed only on 17-2. Further, eight DH lines were evaluated with two CMV isolates (L-BG and PV-0418) and checked for local and systemic presence of the pathogen in leaves and fruits for a period of 60 days by DAS-ELISA. Of the tested DH lines, four (DH2, DH6, DH7 and DH9) were resistant to both strains, two (DH5 and DH14) were resistant to L-BG, and two (DH19 and DH21) were susceptible to both isolates. Field evaluation of DH7, DH9, and DH14 for some agronomic and morphological traits divided them into two groups according to the original genotypes. |
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| language | English |
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| spelling | doaj-art-8edd8dcde17f49ab9d8a1cea6daea2c02025-08-20T02:11:17ZengMDPI AGHorticulturae2311-75242025-03-0111329310.3390/horticulturae11030293Development of Double Haploid Lines from <i>Cucumber mosaic virus</i> Resistant Pepper Germplasm by Anther CultureZhana Ivanova0Gancho Pasev1Veronica Pashkoulova2Vesela Radeva3Stanislava Grozeva4Maritsa Vegetable Crops Research Institute—Plovdiv, Agricultural Academy, 4003 Plovdiv, BulgariaMaritsa Vegetable Crops Research Institute—Plovdiv, Agricultural Academy, 4003 Plovdiv, BulgariaMaritsa Vegetable Crops Research Institute—Plovdiv, Agricultural Academy, 4003 Plovdiv, BulgariaMaritsa Vegetable Crops Research Institute—Plovdiv, Agricultural Academy, 4003 Plovdiv, BulgariaMaritsa Vegetable Crops Research Institute—Plovdiv, Agricultural Academy, 4003 Plovdiv, Bulgaria<i>Cucumber mosaic virus</i> (CMV) is one of the most dangerous viral diseases threatening <i>Solanaceae</i> crops, in particular <i>Capsicum</i> sp. This study aims to develop double haploid (DH) pepper lines from germplasm resistant to CMV in order to speed up the breeding process. For this purpose, six genotypes previously tested for CMV resistance were used. Two induction mediums (17-2 and 17-3) with different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) for anther incubation and further plant regeneration were applied. L10 was the most responsive genotype, exhibiting the highest direct embryogenesis and the most plant regenerants on both mediums. Medium-specific response was observed in genotype L9 where regenerants were observed only on 17-2. Further, eight DH lines were evaluated with two CMV isolates (L-BG and PV-0418) and checked for local and systemic presence of the pathogen in leaves and fruits for a period of 60 days by DAS-ELISA. Of the tested DH lines, four (DH2, DH6, DH7 and DH9) were resistant to both strains, two (DH5 and DH14) were resistant to L-BG, and two (DH19 and DH21) were susceptible to both isolates. Field evaluation of DH7, DH9, and DH14 for some agronomic and morphological traits divided them into two groups according to the original genotypes.https://www.mdpi.com/2311-7524/11/3/293<i>Cucumber mosaic virus</i>double haploidsresistancepepperELISA |
| spellingShingle | Zhana Ivanova Gancho Pasev Veronica Pashkoulova Vesela Radeva Stanislava Grozeva Development of Double Haploid Lines from <i>Cucumber mosaic virus</i> Resistant Pepper Germplasm by Anther Culture Horticulturae <i>Cucumber mosaic virus</i> double haploids resistance pepper ELISA |
| title | Development of Double Haploid Lines from <i>Cucumber mosaic virus</i> Resistant Pepper Germplasm by Anther Culture |
| title_full | Development of Double Haploid Lines from <i>Cucumber mosaic virus</i> Resistant Pepper Germplasm by Anther Culture |
| title_fullStr | Development of Double Haploid Lines from <i>Cucumber mosaic virus</i> Resistant Pepper Germplasm by Anther Culture |
| title_full_unstemmed | Development of Double Haploid Lines from <i>Cucumber mosaic virus</i> Resistant Pepper Germplasm by Anther Culture |
| title_short | Development of Double Haploid Lines from <i>Cucumber mosaic virus</i> Resistant Pepper Germplasm by Anther Culture |
| title_sort | development of double haploid lines from i cucumber mosaic virus i resistant pepper germplasm by anther culture |
| topic | <i>Cucumber mosaic virus</i> double haploids resistance pepper ELISA |
| url | https://www.mdpi.com/2311-7524/11/3/293 |
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