Solid-like condensation of MORF8 inhibits RNA editing under heat stress in Arabidopsis
Abstract Heat stress inhibits photosynthesis efficiency, thereby suppressing plant growth and crop yield. However, the mechanism underlying this inhibition is not fully understood. Here, we report that the multiple organellar RNA-editing factor 8 (MORF8) forms condensates with solid-like properties...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-58146-1 |
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| author | Jie Wu Yue Wang Haodong Chen Tongda Xu Wenqiang Yang Xiaofeng Fang |
| author_facet | Jie Wu Yue Wang Haodong Chen Tongda Xu Wenqiang Yang Xiaofeng Fang |
| author_sort | Jie Wu |
| collection | DOAJ |
| description | Abstract Heat stress inhibits photosynthesis efficiency, thereby suppressing plant growth and crop yield. However, the mechanism underlying this inhibition is not fully understood. Here, we report that the multiple organellar RNA-editing factor 8 (MORF8) forms condensates with solid-like properties in chloroplasts upon heat stress. In vitro data show that the MORF8 condensation is intrinsically heat-dependent and primarily determined by its IDR (intrinsically disordered region). Purification and characterization of MORF8 condensates show that numerous editing factors including PPR proteins and MORFs are partitioned. We provide both genetic and biochemical evidence that MORF8 condensation inhibits chloroplast RNA editing. In agreement, we find that both heat stress and MORF8 condensation lead to reduced editing of RNAs encoding NADH dehydrogenase-like (NDH) complex and impaired NDH activity and photosynthesis efficiency. These findings uncover MORF8 as a putative chloroplastic thermosensor that mediates photosynthesis inhibition by heat and highlight the functional significance of solid material properties of biomolecular condensates. |
| format | Article |
| id | doaj-art-8ed8bcfe6018425394166fbcadbc3310 |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-8ed8bcfe6018425394166fbcadbc33102025-08-20T02:41:34ZengNature PortfolioNature Communications2041-17232025-03-0116111310.1038/s41467-025-58146-1Solid-like condensation of MORF8 inhibits RNA editing under heat stress in ArabidopsisJie Wu0Yue Wang1Haodong Chen2Tongda Xu3Wenqiang Yang4Xiaofeng Fang5Center for Plant Biology, School of Life Sciences, Tsinghua UniversityKey Laboratory of Photobiology, Photosynthesis Research Center, Institute of Botany, Chinese Academy of SciencesCenter for Plant Biology, School of Life Sciences, Tsinghua UniversityHaixia Institute of Science and Technology, and College of Life Sciences, Fujian Agriculture and Forestry UniversityKey Laboratory of Photobiology, Photosynthesis Research Center, Institute of Botany, Chinese Academy of SciencesCenter for Plant Biology, School of Life Sciences, Tsinghua UniversityAbstract Heat stress inhibits photosynthesis efficiency, thereby suppressing plant growth and crop yield. However, the mechanism underlying this inhibition is not fully understood. Here, we report that the multiple organellar RNA-editing factor 8 (MORF8) forms condensates with solid-like properties in chloroplasts upon heat stress. In vitro data show that the MORF8 condensation is intrinsically heat-dependent and primarily determined by its IDR (intrinsically disordered region). Purification and characterization of MORF8 condensates show that numerous editing factors including PPR proteins and MORFs are partitioned. We provide both genetic and biochemical evidence that MORF8 condensation inhibits chloroplast RNA editing. In agreement, we find that both heat stress and MORF8 condensation lead to reduced editing of RNAs encoding NADH dehydrogenase-like (NDH) complex and impaired NDH activity and photosynthesis efficiency. These findings uncover MORF8 as a putative chloroplastic thermosensor that mediates photosynthesis inhibition by heat and highlight the functional significance of solid material properties of biomolecular condensates.https://doi.org/10.1038/s41467-025-58146-1 |
| spellingShingle | Jie Wu Yue Wang Haodong Chen Tongda Xu Wenqiang Yang Xiaofeng Fang Solid-like condensation of MORF8 inhibits RNA editing under heat stress in Arabidopsis Nature Communications |
| title | Solid-like condensation of MORF8 inhibits RNA editing under heat stress in Arabidopsis |
| title_full | Solid-like condensation of MORF8 inhibits RNA editing under heat stress in Arabidopsis |
| title_fullStr | Solid-like condensation of MORF8 inhibits RNA editing under heat stress in Arabidopsis |
| title_full_unstemmed | Solid-like condensation of MORF8 inhibits RNA editing under heat stress in Arabidopsis |
| title_short | Solid-like condensation of MORF8 inhibits RNA editing under heat stress in Arabidopsis |
| title_sort | solid like condensation of morf8 inhibits rna editing under heat stress in arabidopsis |
| url | https://doi.org/10.1038/s41467-025-58146-1 |
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