Simultaneous Component Analysis of <i>Akebia quinata</i> Seeds (Lardizabalaceae) by Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry for Quality and Cytotoxicity Assessment
<i>Akebia quinata</i> seeds (AQSs) are used as an analgesic, antiphlogistic, and diuretic in traditional herbal medicine. We developed an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC–MS/MS) simultaneous component analysis method to analyze eight compounds (...
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2025-02-01
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| author | Chang-Seob Seo Jaemoo Chun Kwang Hoon Song |
| author_facet | Chang-Seob Seo Jaemoo Chun Kwang Hoon Song |
| author_sort | Chang-Seob Seo |
| collection | DOAJ |
| description | <i>Akebia quinata</i> seeds (AQSs) are used as an analgesic, antiphlogistic, and diuretic in traditional herbal medicine. We developed an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC–MS/MS) simultaneous component analysis method to analyze eight compounds (chlorogenic acid, isochlorogenic acid A, isochlorogenic acid C, hederacolchiside F, hederacoside C, dipsacoside B, akebia saponin D, and α-hederin) as markers for the quality assessment of AQSs. The separation of the eight analytes was performed in an Acquity UPLC BEH C<sub>18</sub> reversed-phase analytical column. The method was validated with respect to linearity (coefficient of determination ≥ 0.994), recovery (90.32–108.18%; relative standard deviation (RSD) < 10.0%), and precision (RSD < 10%). The analysis of the AQSs confirmed that the eight components were found in concentrations of 0.42–9.07 mg/g. The cytotoxicity of the AQS extract and the eight compounds against human cancer cell lines, including MDA-MB-231 (breast), A549 (lung), HCT 116 (colon), AsPC-1 (pancreas), and A2780 (ovarian), was also assessed, with cisplatin used as a positive control. In addition, dipsacoside B showed high cytotoxicity in all cell lines. This assay will help to enhance efficacy and clinical research as well as provide a validated quality assessment of AQS extract and related traditional herbal medicines. |
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| language | English |
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| spelling | doaj-art-8ed6d6a150804782bdc925efb017e76e2025-08-20T02:59:15ZengMDPI AGPlants2223-77472025-02-0114566910.3390/plants14050669Simultaneous Component Analysis of <i>Akebia quinata</i> Seeds (Lardizabalaceae) by Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry for Quality and Cytotoxicity AssessmentChang-Seob Seo0Jaemoo Chun1Kwang Hoon Song2KM Science Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Republic of KoreaKM Convergence Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Republic of KoreaKM Convergence Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Republic of Korea<i>Akebia quinata</i> seeds (AQSs) are used as an analgesic, antiphlogistic, and diuretic in traditional herbal medicine. We developed an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC–MS/MS) simultaneous component analysis method to analyze eight compounds (chlorogenic acid, isochlorogenic acid A, isochlorogenic acid C, hederacolchiside F, hederacoside C, dipsacoside B, akebia saponin D, and α-hederin) as markers for the quality assessment of AQSs. The separation of the eight analytes was performed in an Acquity UPLC BEH C<sub>18</sub> reversed-phase analytical column. The method was validated with respect to linearity (coefficient of determination ≥ 0.994), recovery (90.32–108.18%; relative standard deviation (RSD) < 10.0%), and precision (RSD < 10%). The analysis of the AQSs confirmed that the eight components were found in concentrations of 0.42–9.07 mg/g. The cytotoxicity of the AQS extract and the eight compounds against human cancer cell lines, including MDA-MB-231 (breast), A549 (lung), HCT 116 (colon), AsPC-1 (pancreas), and A2780 (ovarian), was also assessed, with cisplatin used as a positive control. In addition, dipsacoside B showed high cytotoxicity in all cell lines. This assay will help to enhance efficacy and clinical research as well as provide a validated quality assessment of AQS extract and related traditional herbal medicines.https://www.mdpi.com/2223-7747/14/5/669simultaneous determination<i>Akebia quinate</i> seedsquality assessmentUPLC–MS/MScytotoxicity |
| spellingShingle | Chang-Seob Seo Jaemoo Chun Kwang Hoon Song Simultaneous Component Analysis of <i>Akebia quinata</i> Seeds (Lardizabalaceae) by Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry for Quality and Cytotoxicity Assessment Plants simultaneous determination <i>Akebia quinate</i> seeds quality assessment UPLC–MS/MS cytotoxicity |
| title | Simultaneous Component Analysis of <i>Akebia quinata</i> Seeds (Lardizabalaceae) by Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry for Quality and Cytotoxicity Assessment |
| title_full | Simultaneous Component Analysis of <i>Akebia quinata</i> Seeds (Lardizabalaceae) by Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry for Quality and Cytotoxicity Assessment |
| title_fullStr | Simultaneous Component Analysis of <i>Akebia quinata</i> Seeds (Lardizabalaceae) by Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry for Quality and Cytotoxicity Assessment |
| title_full_unstemmed | Simultaneous Component Analysis of <i>Akebia quinata</i> Seeds (Lardizabalaceae) by Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry for Quality and Cytotoxicity Assessment |
| title_short | Simultaneous Component Analysis of <i>Akebia quinata</i> Seeds (Lardizabalaceae) by Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry for Quality and Cytotoxicity Assessment |
| title_sort | simultaneous component analysis of i akebia quinata i seeds lardizabalaceae by ultra performance liquid chromatography tandem mass spectrometry for quality and cytotoxicity assessment |
| topic | simultaneous determination <i>Akebia quinate</i> seeds quality assessment UPLC–MS/MS cytotoxicity |
| url | https://www.mdpi.com/2223-7747/14/5/669 |
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