Simultaneous Component Analysis of <i>Akebia quinata</i> Seeds (Lardizabalaceae) by Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry for Quality and Cytotoxicity Assessment
<i>Akebia quinata</i> seeds (AQSs) are used as an analgesic, antiphlogistic, and diuretic in traditional herbal medicine. We developed an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC–MS/MS) simultaneous component analysis method to analyze eight compounds (...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
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| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/5/669 |
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| Summary: | <i>Akebia quinata</i> seeds (AQSs) are used as an analgesic, antiphlogistic, and diuretic in traditional herbal medicine. We developed an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC–MS/MS) simultaneous component analysis method to analyze eight compounds (chlorogenic acid, isochlorogenic acid A, isochlorogenic acid C, hederacolchiside F, hederacoside C, dipsacoside B, akebia saponin D, and α-hederin) as markers for the quality assessment of AQSs. The separation of the eight analytes was performed in an Acquity UPLC BEH C<sub>18</sub> reversed-phase analytical column. The method was validated with respect to linearity (coefficient of determination ≥ 0.994), recovery (90.32–108.18%; relative standard deviation (RSD) < 10.0%), and precision (RSD < 10%). The analysis of the AQSs confirmed that the eight components were found in concentrations of 0.42–9.07 mg/g. The cytotoxicity of the AQS extract and the eight compounds against human cancer cell lines, including MDA-MB-231 (breast), A549 (lung), HCT 116 (colon), AsPC-1 (pancreas), and A2780 (ovarian), was also assessed, with cisplatin used as a positive control. In addition, dipsacoside B showed high cytotoxicity in all cell lines. This assay will help to enhance efficacy and clinical research as well as provide a validated quality assessment of AQS extract and related traditional herbal medicines. |
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| ISSN: | 2223-7747 |