MOLECULAR MARKING OF SUNFLOWER LINES WITH DIFFERENT ABILITY TO SUPPRESSION OF THE CYTOPLASMIC MALE STERILITY PHENOTYPE
Ninety five lines of sunflower genetic collection differing by their ability to suppress the CMS phenotype were molecularly marked with the use of 7 primer pairs. Using the STS marker orfH522, a sterile (PET1) cytoplasmon was identified in 79 lines, which confirmed indirectly the presence of fertili...
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| Format: | Article |
| Language: | English |
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N.I. Vavilov All-Russian Institute of Plant Genetic Resources
2018-06-01
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| Series: | Труды по прикладной ботанике, генетике и селекции |
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| Online Access: | https://elpub.vir.nw.ru/jour/article/view/82 |
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| author | Yu. I. Karabitsina I. N. Anisimova V. A. Gavrilova N. V. Alpatieva A. G. Pinaev E. B. Kuznetsova V. T. Rozhkova |
| author_facet | Yu. I. Karabitsina I. N. Anisimova V. A. Gavrilova N. V. Alpatieva A. G. Pinaev E. B. Kuznetsova V. T. Rozhkova |
| author_sort | Yu. I. Karabitsina |
| collection | DOAJ |
| description | Ninety five lines of sunflower genetic collection differing by their ability to suppress the CMS phenotype were molecularly marked with the use of 7 primer pairs. Using the STS marker orfH522, a sterile (PET1) cytoplasmon was identified in 79 lines, which confirmed indirectly the presence of fertility restoration genes in their genotypes. The majority of these lines also have a complex of molecular markers linked to the Rf1 gene. The HRG01, HRG02 and STS115 markers showed the best diagnostic value in revealing the Rf1 gene in the examined material. The data on allelic variation of the microsatellite loci ORS224, ORS511 and ORS799 were obtained for the first time. |
| format | Article |
| id | doaj-art-8ed4e8a412b548419ede5f6998bb6ed7 |
| institution | Kabale University |
| issn | 2227-8834 2619-0982 |
| language | English |
| publishDate | 2018-06-01 |
| publisher | N.I. Vavilov All-Russian Institute of Plant Genetic Resources |
| record_format | Article |
| series | Труды по прикладной ботанике, генетике и селекции |
| spelling | doaj-art-8ed4e8a412b548419ede5f6998bb6ed72025-02-03T08:31:10ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesТруды по прикладной ботанике, генетике и селекции2227-88342619-09822018-06-0117729910710.30901/2227-8834-2016-2-99-10782MOLECULAR MARKING OF SUNFLOWER LINES WITH DIFFERENT ABILITY TO SUPPRESSION OF THE CYTOPLASMIC MALE STERILITY PHENOTYPEYu. I. Karabitsina0I. N. Anisimova1V. A. Gavrilova2N. V. Alpatieva3A. G. Pinaev4E. B. Kuznetsova5V. T. Rozhkova6Federal Research Center the N.I.Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Federal Research Center the N.I.Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Federal Research Center the N.I.Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Federal Research Center the N.I.Vavilov All-Russian Institute of Plant Genetic Resources (VIR)All-Russian Research Institute for Agricultural MicrobiologyFederal Research Center the N.I.Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Federal Research Center the N.I.Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Ninety five lines of sunflower genetic collection differing by their ability to suppress the CMS phenotype were molecularly marked with the use of 7 primer pairs. Using the STS marker orfH522, a sterile (PET1) cytoplasmon was identified in 79 lines, which confirmed indirectly the presence of fertility restoration genes in their genotypes. The majority of these lines also have a complex of molecular markers linked to the Rf1 gene. The HRG01, HRG02 and STS115 markers showed the best diagnostic value in revealing the Rf1 gene in the examined material. The data on allelic variation of the microsatellite loci ORS224, ORS511 and ORS799 were obtained for the first time.https://elpub.vir.nw.ru/jour/article/view/82генетикаподсолнечниклиниигенетическая коллекцияцмсвосстановление фертильности пыльцыгенымолекулярные маркерыgeneticsunflowerlinesgenetic collectioncmspollen fertility restorationgenesmolecular markers |
| spellingShingle | Yu. I. Karabitsina I. N. Anisimova V. A. Gavrilova N. V. Alpatieva A. G. Pinaev E. B. Kuznetsova V. T. Rozhkova MOLECULAR MARKING OF SUNFLOWER LINES WITH DIFFERENT ABILITY TO SUPPRESSION OF THE CYTOPLASMIC MALE STERILITY PHENOTYPE Труды по прикладной ботанике, генетике и селекции генетика подсолнечник линии генетическая коллекция цмс восстановление фертильности пыльцы гены молекулярные маркеры genetic sunflower lines genetic collection cms pollen fertility restoration genes molecular markers |
| title | MOLECULAR MARKING OF SUNFLOWER LINES WITH DIFFERENT ABILITY TO SUPPRESSION OF THE CYTOPLASMIC MALE STERILITY PHENOTYPE |
| title_full | MOLECULAR MARKING OF SUNFLOWER LINES WITH DIFFERENT ABILITY TO SUPPRESSION OF THE CYTOPLASMIC MALE STERILITY PHENOTYPE |
| title_fullStr | MOLECULAR MARKING OF SUNFLOWER LINES WITH DIFFERENT ABILITY TO SUPPRESSION OF THE CYTOPLASMIC MALE STERILITY PHENOTYPE |
| title_full_unstemmed | MOLECULAR MARKING OF SUNFLOWER LINES WITH DIFFERENT ABILITY TO SUPPRESSION OF THE CYTOPLASMIC MALE STERILITY PHENOTYPE |
| title_short | MOLECULAR MARKING OF SUNFLOWER LINES WITH DIFFERENT ABILITY TO SUPPRESSION OF THE CYTOPLASMIC MALE STERILITY PHENOTYPE |
| title_sort | molecular marking of sunflower lines with different ability to suppression of the cytoplasmic male sterility phenotype |
| topic | генетика подсолнечник линии генетическая коллекция цмс восстановление фертильности пыльцы гены молекулярные маркеры genetic sunflower lines genetic collection cms pollen fertility restoration genes molecular markers |
| url | https://elpub.vir.nw.ru/jour/article/view/82 |
| work_keys_str_mv | AT yuikarabitsina molecularmarkingofsunflowerlineswithdifferentabilitytosuppressionofthecytoplasmicmalesterilityphenotype AT inanisimova molecularmarkingofsunflowerlineswithdifferentabilitytosuppressionofthecytoplasmicmalesterilityphenotype AT vagavrilova molecularmarkingofsunflowerlineswithdifferentabilitytosuppressionofthecytoplasmicmalesterilityphenotype AT nvalpatieva molecularmarkingofsunflowerlineswithdifferentabilitytosuppressionofthecytoplasmicmalesterilityphenotype AT agpinaev molecularmarkingofsunflowerlineswithdifferentabilitytosuppressionofthecytoplasmicmalesterilityphenotype AT ebkuznetsova molecularmarkingofsunflowerlineswithdifferentabilitytosuppressionofthecytoplasmicmalesterilityphenotype AT vtrozhkova molecularmarkingofsunflowerlineswithdifferentabilitytosuppressionofthecytoplasmicmalesterilityphenotype |