Forskolin-containing conditioned medium induces neural differentiation of adipose-derived stem cells: An in vitro experimental study
[Objective:] To explore the neural differentiation of adipose-derived stem cells (ASCs) induced by neural-induced conditional medium in vitro, and to study the optimal concentration of forskolin and the effect of optimal concentration on neurogenic differentiation of ASCs in vitro. [Methods:] ASCs w...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Oral and Maxillofacial Surgery
2024-06-01
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| Series: | Kouqiang hemian waike zazhi |
| Subjects: | |
| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2024.03.002 |
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| Summary: | [Objective:] To explore the neural differentiation of adipose-derived stem cells (ASCs) induced by neural-induced conditional medium in vitro, and to study the optimal concentration of forskolin and the effect of optimal concentration on neurogenic differentiation of ASCs in vitro. [Methods:] ASCs were isolated by enzyme digestion method. The phenotype and differentiation ability of stem cells are identified by flow cytometry and cell staining. Nerve induction differentiation was performed in vitro by neural-induced conditional medium containing different concentrations of forskolin, and the optimal concentration was screened by immunofluorescence staining. ASCs were induced by neural-induced conditional medium containing the optimal concentration of forskolin and the morphological changes of cells were observed by microscope before induction, 6 h after induction and 24 h after induction. Effects of induction on rat ASCs were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR) and immunofluorescence. [Results:] The optimal concentration of forskolin in neural-induced conditional medium was 10 μmol/L, and the cells induced by neural-induced conditional medium containing 10 μmol/L forskolin could rapidly induce ASCs to differentiate in the direction of nerve formation. After induction, the cell morphology was similar to nerve cells. Results of RT-qPCR and immunofluorescence indicated that the expressions of anti-class Ⅲ β-tubulin (Tuj1), neurofilament-M (NF-M), nestin and S100 β at the mRNA level and protein level were increased after induction (P<0.05). [Conclusion:] Nerve differentiation of ASCs can be induced by conditional medium containing 10 μmol/L forskolin, and the induced cells are expected to become seed cells of facial nerve conduits. |
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| ISSN: | 1005-4979 |