High throughput sequencing sheds light on the viral diversity in Aedes aegypti mosquitoes from Binh Thuan Province, Vietnam.

Mosquitoes are important vectors for various infectious pathogens. More than 200 species of mosquitoes are common in Vietnam, one of the main carriers of viruses that are important for humans is the mosquito of the genus Aedes aegypti. Metavirome sequencing can shed light on the diversity of mosquit...

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Main Authors: Margarita Popova, Anna Gladkikh, Alena Sharova, Tatiana Arbuzova, Valeriya Sbarzaglia, Ekaterina Klyuchnikova, Nadezhda Tsyganova, Majid Forghani, Anastasia Gritseva, Edward Ramsay, Nguyen Thanh Dong, Bui Thanh Phu, Do Thai Hung, Vladimir Dedkov
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0322924
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author Margarita Popova
Anna Gladkikh
Alena Sharova
Tatiana Arbuzova
Valeriya Sbarzaglia
Ekaterina Klyuchnikova
Nadezhda Tsyganova
Majid Forghani
Anastasia Gritseva
Edward Ramsay
Nguyen Thanh Dong
Bui Thanh Phu
Do Thai Hung
Vladimir Dedkov
author_facet Margarita Popova
Anna Gladkikh
Alena Sharova
Tatiana Arbuzova
Valeriya Sbarzaglia
Ekaterina Klyuchnikova
Nadezhda Tsyganova
Majid Forghani
Anastasia Gritseva
Edward Ramsay
Nguyen Thanh Dong
Bui Thanh Phu
Do Thai Hung
Vladimir Dedkov
author_sort Margarita Popova
collection DOAJ
description Mosquitoes are important vectors for various infectious pathogens. More than 200 species of mosquitoes are common in Vietnam, one of the main carriers of viruses that are important for humans is the mosquito of the genus Aedes aegypti. Metavirome sequencing can shed light on the diversity of mosquito-borne viruses classified as insect-specific viruses (ISV). After BLAST analysis using the viral database, contigs were classified as belonging to seventeen ISVs. Ten of them are distributed among five families: Totiviridae; Flaviviridae; Partitiviridae; Phenuiviridae; and Orthomyxoviridae. The other seven species belonged to recently identified RNA viruses whose taxonomic position is undefined in the current classification of the International Committee on Taxonomy of Viruses (ICTV). This is the first study to reveal the diversity of RNA viruses associated with Aedes aegypti mosquitoes in Vietnam, while highlighting the need for further study of ISV in mosquito vectors.
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publisher Public Library of Science (PLoS)
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spelling doaj-art-8eb81585209a4031b1344f28ca1e3bb42025-08-20T03:08:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01205e032292410.1371/journal.pone.0322924High throughput sequencing sheds light on the viral diversity in Aedes aegypti mosquitoes from Binh Thuan Province, Vietnam.Margarita PopovaAnna GladkikhAlena SharovaTatiana ArbuzovaValeriya SbarzagliaEkaterina KlyuchnikovaNadezhda TsyganovaMajid ForghaniAnastasia GritsevaEdward RamsayNguyen Thanh DongBui Thanh PhuDo Thai HungVladimir DedkovMosquitoes are important vectors for various infectious pathogens. More than 200 species of mosquitoes are common in Vietnam, one of the main carriers of viruses that are important for humans is the mosquito of the genus Aedes aegypti. Metavirome sequencing can shed light on the diversity of mosquito-borne viruses classified as insect-specific viruses (ISV). After BLAST analysis using the viral database, contigs were classified as belonging to seventeen ISVs. Ten of them are distributed among five families: Totiviridae; Flaviviridae; Partitiviridae; Phenuiviridae; and Orthomyxoviridae. The other seven species belonged to recently identified RNA viruses whose taxonomic position is undefined in the current classification of the International Committee on Taxonomy of Viruses (ICTV). This is the first study to reveal the diversity of RNA viruses associated with Aedes aegypti mosquitoes in Vietnam, while highlighting the need for further study of ISV in mosquito vectors.https://doi.org/10.1371/journal.pone.0322924
spellingShingle Margarita Popova
Anna Gladkikh
Alena Sharova
Tatiana Arbuzova
Valeriya Sbarzaglia
Ekaterina Klyuchnikova
Nadezhda Tsyganova
Majid Forghani
Anastasia Gritseva
Edward Ramsay
Nguyen Thanh Dong
Bui Thanh Phu
Do Thai Hung
Vladimir Dedkov
High throughput sequencing sheds light on the viral diversity in Aedes aegypti mosquitoes from Binh Thuan Province, Vietnam.
PLoS ONE
title High throughput sequencing sheds light on the viral diversity in Aedes aegypti mosquitoes from Binh Thuan Province, Vietnam.
title_full High throughput sequencing sheds light on the viral diversity in Aedes aegypti mosquitoes from Binh Thuan Province, Vietnam.
title_fullStr High throughput sequencing sheds light on the viral diversity in Aedes aegypti mosquitoes from Binh Thuan Province, Vietnam.
title_full_unstemmed High throughput sequencing sheds light on the viral diversity in Aedes aegypti mosquitoes from Binh Thuan Province, Vietnam.
title_short High throughput sequencing sheds light on the viral diversity in Aedes aegypti mosquitoes from Binh Thuan Province, Vietnam.
title_sort high throughput sequencing sheds light on the viral diversity in aedes aegypti mosquitoes from binh thuan province vietnam
url https://doi.org/10.1371/journal.pone.0322924
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