Cytokeratin-1 is essential for the detection of laminar shear stress in endothelial cells
Endothelial cells regulate diverse vascular functions by perceiving and reacting to laminar shear stress. In this study, a novel shear-sensing receptor was identified through the use of a pro-inflammatory protein, lysyl-tRNA synthetase (KARS), which is known to be secreted from endothelial cells via...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Animal Cells and Systems |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2025.2526426 |
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| author | Sunyoung Ahn Youngsik Seo Heonyong Park |
| author_facet | Sunyoung Ahn Youngsik Seo Heonyong Park |
| author_sort | Sunyoung Ahn |
| collection | DOAJ |
| description | Endothelial cells regulate diverse vascular functions by perceiving and reacting to laminar shear stress. In this study, a novel shear-sensing receptor was identified through the use of a pro-inflammatory protein, lysyl-tRNA synthetase (KARS), which is known to be secreted from endothelial cells via autophagy. Binding assays demonstrated that cytokeratin-1 (CK1) interacts with KARS at the endothelial cell surface. Additionally, CK1 was shown to be critical for ECM-cell adhesion and endothelial sensing of shear stress by mediating interactions with laminin and integrin α6. Overexpression of CK1 results in hyperactivation of endothelial nitric oxide synthase (eNOS) in response to laminar shear stress (LSS), potentially reducing the risk of atherosclerosis. Furthermore, elevated CK1 expression significantly decreases leukocyte adhesion to endothelial cells by modulating nitric oxide production stimulated by LSS. Conversely, CK1 knockdown leads to the formation of actin fibers and diminishes LSS-induced activation of several cell signaling components. These findings indicate that CK1 is a shear-sensing receptor, providing new perspectives on the close relationship between cell-to-matrix adhesion and mechanosensing. |
| format | Article |
| id | doaj-art-8eb7154e187043ceb50fbc34dd88e210 |
| institution | DOAJ |
| issn | 1976-8354 2151-2485 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj-art-8eb7154e187043ceb50fbc34dd88e2102025-08-20T03:17:23ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852025-12-0129142643710.1080/19768354.2025.2526426Cytokeratin-1 is essential for the detection of laminar shear stress in endothelial cellsSunyoung Ahn0Youngsik Seo1Heonyong Park2Department of Biological Science & Institute of Nanosensor and Biotechnology, Dankook University, Cheonan-si, Chungnam, South KoreaDepartment of Biological Science & Institute of Nanosensor and Biotechnology, Dankook University, Cheonan-si, Chungnam, South KoreaDepartment of Biological Science & Institute of Nanosensor and Biotechnology, Dankook University, Cheonan-si, Chungnam, South KoreaEndothelial cells regulate diverse vascular functions by perceiving and reacting to laminar shear stress. In this study, a novel shear-sensing receptor was identified through the use of a pro-inflammatory protein, lysyl-tRNA synthetase (KARS), which is known to be secreted from endothelial cells via autophagy. Binding assays demonstrated that cytokeratin-1 (CK1) interacts with KARS at the endothelial cell surface. Additionally, CK1 was shown to be critical for ECM-cell adhesion and endothelial sensing of shear stress by mediating interactions with laminin and integrin α6. Overexpression of CK1 results in hyperactivation of endothelial nitric oxide synthase (eNOS) in response to laminar shear stress (LSS), potentially reducing the risk of atherosclerosis. Furthermore, elevated CK1 expression significantly decreases leukocyte adhesion to endothelial cells by modulating nitric oxide production stimulated by LSS. Conversely, CK1 knockdown leads to the formation of actin fibers and diminishes LSS-induced activation of several cell signaling components. These findings indicate that CK1 is a shear-sensing receptor, providing new perspectives on the close relationship between cell-to-matrix adhesion and mechanosensing.https://www.tandfonline.com/doi/10.1080/19768354.2025.2526426Cytokeratin-1laminar shear stresscell-to-matrix adhesionaminoacyl tRNA synthetasesignal transductions |
| spellingShingle | Sunyoung Ahn Youngsik Seo Heonyong Park Cytokeratin-1 is essential for the detection of laminar shear stress in endothelial cells Animal Cells and Systems Cytokeratin-1 laminar shear stress cell-to-matrix adhesion aminoacyl tRNA synthetase signal transductions |
| title | Cytokeratin-1 is essential for the detection of laminar shear stress in endothelial cells |
| title_full | Cytokeratin-1 is essential for the detection of laminar shear stress in endothelial cells |
| title_fullStr | Cytokeratin-1 is essential for the detection of laminar shear stress in endothelial cells |
| title_full_unstemmed | Cytokeratin-1 is essential for the detection of laminar shear stress in endothelial cells |
| title_short | Cytokeratin-1 is essential for the detection of laminar shear stress in endothelial cells |
| title_sort | cytokeratin 1 is essential for the detection of laminar shear stress in endothelial cells |
| topic | Cytokeratin-1 laminar shear stress cell-to-matrix adhesion aminoacyl tRNA synthetase signal transductions |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2025.2526426 |
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