Gut microbiome signature in response to neoadjuvant chemoradiotherapy in patients with rectal cancer
BackgroundRectal cancer remains a leading cause of cancer-associated mortality, especially in advanced cases with limited treatment options. Emerging evidence suggests that the gut microbiome may influence the therapeutic efficacy of neoadjuvant chemoradiotherapy (CRT).ObjectiveThis study aimed to e...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1543507/full |
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| author | Tingmei Duan Zhengting Ren Haili Jiang Yan Ding Hongyan Wang Fan Wang |
| author_facet | Tingmei Duan Zhengting Ren Haili Jiang Yan Ding Hongyan Wang Fan Wang |
| author_sort | Tingmei Duan |
| collection | DOAJ |
| description | BackgroundRectal cancer remains a leading cause of cancer-associated mortality, especially in advanced cases with limited treatment options. Emerging evidence suggests that the gut microbiome may influence the therapeutic efficacy of neoadjuvant chemoradiotherapy (CRT).ObjectiveThis study aimed to explore the dynamic changes in gut microbiome composition and metabolic pathways in rectal cancer patients undergoing CRT.MethodsPaired fecal samples were collected from rectal cancer patients pre- and post-CRT. 16S rRNA amplicon sequencing and proteomics analysis were conducted to investigate microbial and metabolic alterations.ResultsSignificant shifts in the microbiome were observed, with Fusobacterium, Subdoligranulum, Prevotella, Alloprevotella, and Bacteroides being enriched pre-CRT, while Streptococcus, Megamonas, Megasphaera, Escherichia-Shigella, and Olsenella became dominant post-CRT. Metabolic analysis revealed upregulated carbohydrate metabolism and downregulated lipid and energy metabolism.ConclusionThese findings identify potential microbial biomarkers and metabolic pathways associated with CRT response, offering insights into personalized treatment strategies. |
| format | Article |
| id | doaj-art-8ea7adadd7da4dfaa38d88ce191232ad |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-8ea7adadd7da4dfaa38d88ce191232ad2025-08-20T02:08:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-04-011610.3389/fmicb.2025.15435071543507Gut microbiome signature in response to neoadjuvant chemoradiotherapy in patients with rectal cancerTingmei Duan0Zhengting Ren1Haili Jiang2Yan Ding3Hongyan Wang4Fan Wang5Department of Radiation Oncology, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Radiation Oncology, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Integrated Chinese and Western Medicine Oncology, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Radiation Oncology, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Radiation Oncology, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaDepartment of Radiation Oncology, First Affiliated Hospital of Anhui Medical University, Hefei, ChinaBackgroundRectal cancer remains a leading cause of cancer-associated mortality, especially in advanced cases with limited treatment options. Emerging evidence suggests that the gut microbiome may influence the therapeutic efficacy of neoadjuvant chemoradiotherapy (CRT).ObjectiveThis study aimed to explore the dynamic changes in gut microbiome composition and metabolic pathways in rectal cancer patients undergoing CRT.MethodsPaired fecal samples were collected from rectal cancer patients pre- and post-CRT. 16S rRNA amplicon sequencing and proteomics analysis were conducted to investigate microbial and metabolic alterations.ResultsSignificant shifts in the microbiome were observed, with Fusobacterium, Subdoligranulum, Prevotella, Alloprevotella, and Bacteroides being enriched pre-CRT, while Streptococcus, Megamonas, Megasphaera, Escherichia-Shigella, and Olsenella became dominant post-CRT. Metabolic analysis revealed upregulated carbohydrate metabolism and downregulated lipid and energy metabolism.ConclusionThese findings identify potential microbial biomarkers and metabolic pathways associated with CRT response, offering insights into personalized treatment strategies.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1543507/fullrectal cancerneoadjuvant chemoradiotherapy (CRT)gut microbiomeproteomics16S rRNA sequencing |
| spellingShingle | Tingmei Duan Zhengting Ren Haili Jiang Yan Ding Hongyan Wang Fan Wang Gut microbiome signature in response to neoadjuvant chemoradiotherapy in patients with rectal cancer Frontiers in Microbiology rectal cancer neoadjuvant chemoradiotherapy (CRT) gut microbiome proteomics 16S rRNA sequencing |
| title | Gut microbiome signature in response to neoadjuvant chemoradiotherapy in patients with rectal cancer |
| title_full | Gut microbiome signature in response to neoadjuvant chemoradiotherapy in patients with rectal cancer |
| title_fullStr | Gut microbiome signature in response to neoadjuvant chemoradiotherapy in patients with rectal cancer |
| title_full_unstemmed | Gut microbiome signature in response to neoadjuvant chemoradiotherapy in patients with rectal cancer |
| title_short | Gut microbiome signature in response to neoadjuvant chemoradiotherapy in patients with rectal cancer |
| title_sort | gut microbiome signature in response to neoadjuvant chemoradiotherapy in patients with rectal cancer |
| topic | rectal cancer neoadjuvant chemoradiotherapy (CRT) gut microbiome proteomics 16S rRNA sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1543507/full |
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