Regulation of Muscle Quality of Tilapia by Embrittlement

This study systematically evaluated the effects of embrittling duration on the quality of tilapia muscle through sensory evaluation, physicochemical analysis, microstructural observation, and myofibrillar protein (MP) structure analysis. The results demonstrated that prolonged embrittling time signi...

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Main Authors: Junmeng YAN, Sili XIAO, Qiaoyu LIU, Weidong BAI, Ting WU, Qingxiu WANG, Ling YANG, Baiqiao OU
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2025-08-01
Series:Shipin gongye ke-ji
Subjects:
Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070129
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author Junmeng YAN
Sili XIAO
Qiaoyu LIU
Weidong BAI
Ting WU
Qingxiu WANG
Ling YANG
Baiqiao OU
author_facet Junmeng YAN
Sili XIAO
Qiaoyu LIU
Weidong BAI
Ting WU
Qingxiu WANG
Ling YANG
Baiqiao OU
author_sort Junmeng YAN
collection DOAJ
description This study systematically evaluated the effects of embrittling duration on the quality of tilapia muscle through sensory evaluation, physicochemical analysis, microstructural observation, and myofibrillar protein (MP) structure analysis. The results demonstrated that prolonged embrittling time significantly enhanced muscle hardness, springiness, adhesiveness, and chewiness (P<0.05). Water-holding capacity improved by 6.47% (embrittlement for 90 days), while water distribution analysis revealed decreased free water content and increased bound water content (P<0.05). Microstructural observations indicated that extended embrittling time led to tighter myofibrillar connections and significantly enhanced connective tissue and collagen fibers (P<0.05). In the MP structure, total sulfhydryl content significantly decreased, hydrophobicity increased, α-helix relative content reduced, and β-sheet relative content elevated (P<0.05). Additionally, fluorescence intensity declined, accompanied by a blue shift in the emission wavelength. SDS-PAGE analysis showed that embrittling treatment weakened the expression of MHC, AC, and TM bands and formed high-molecular-weight aggregates. Correlation analysis revealed that the proportion of bound water in muscle, connective tissue proliferation, protein hydrophobic interactions, and intermolecular forces were positively correlated with muscle quality attributes, including hardness, springiness, adhesiveness, and chewiness. Overall, tilapia muscle embrittled for 90 days exhibited optimal sensory attributes, texture, and water distribution, suggesting that 90 days was the recommended duration for embrittling in production.
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institution Kabale University
issn 1002-0306
language zho
publishDate 2025-08-01
publisher The editorial department of Science and Technology of Food Industry
record_format Article
series Shipin gongye ke-ji
spelling doaj-art-8ea58880d8b144409fef5e3bb3d1a74e2025-08-20T03:43:55ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-08-014615768510.13386/j.issn1002-0306.20240701292024070129-15Regulation of Muscle Quality of Tilapia by EmbrittlementJunmeng YAN0Sili XIAO1Qiaoyu LIU2Weidong BAI3Ting WU4Qingxiu WANG5Ling YANG6Baiqiao OU7School of Light Industry and Food, Zhongkai College of Agricultural Engineering, Guangzhou 510225, ChinaSchool of Light Industry and Food, Zhongkai College of Agricultural Engineering, Guangzhou 510225, ChinaSchool of Light Industry and Food, Zhongkai College of Agricultural Engineering, Guangzhou 510225, ChinaSchool of Light Industry and Food, Zhongkai College of Agricultural Engineering, Guangzhou 510225, ChinaSchool of Information Science and Technology, Zhongkai College of Agricultural Engineering, Guangzhou 510225, ChinaSchool of Information Science and Technology, Zhongkai College of Agricultural Engineering, Guangzhou 510225, ChinaSchool of Information Science and Technology, Zhongkai College of Agricultural Engineering, Guangzhou 510225, ChinaMaoming Dayu Aquatic Products Co., Ltd., Maoming 525000, ChinaThis study systematically evaluated the effects of embrittling duration on the quality of tilapia muscle through sensory evaluation, physicochemical analysis, microstructural observation, and myofibrillar protein (MP) structure analysis. The results demonstrated that prolonged embrittling time significantly enhanced muscle hardness, springiness, adhesiveness, and chewiness (P<0.05). Water-holding capacity improved by 6.47% (embrittlement for 90 days), while water distribution analysis revealed decreased free water content and increased bound water content (P<0.05). Microstructural observations indicated that extended embrittling time led to tighter myofibrillar connections and significantly enhanced connective tissue and collagen fibers (P<0.05). In the MP structure, total sulfhydryl content significantly decreased, hydrophobicity increased, α-helix relative content reduced, and β-sheet relative content elevated (P<0.05). Additionally, fluorescence intensity declined, accompanied by a blue shift in the emission wavelength. SDS-PAGE analysis showed that embrittling treatment weakened the expression of MHC, AC, and TM bands and formed high-molecular-weight aggregates. Correlation analysis revealed that the proportion of bound water in muscle, connective tissue proliferation, protein hydrophobic interactions, and intermolecular forces were positively correlated with muscle quality attributes, including hardness, springiness, adhesiveness, and chewiness. Overall, tilapia muscle embrittled for 90 days exhibited optimal sensory attributes, texture, and water distribution, suggesting that 90 days was the recommended duration for embrittling in production.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070129embrittlementtilapiamuscle qualityregulation
spellingShingle Junmeng YAN
Sili XIAO
Qiaoyu LIU
Weidong BAI
Ting WU
Qingxiu WANG
Ling YANG
Baiqiao OU
Regulation of Muscle Quality of Tilapia by Embrittlement
Shipin gongye ke-ji
embrittlement
tilapia
muscle quality
regulation
title Regulation of Muscle Quality of Tilapia by Embrittlement
title_full Regulation of Muscle Quality of Tilapia by Embrittlement
title_fullStr Regulation of Muscle Quality of Tilapia by Embrittlement
title_full_unstemmed Regulation of Muscle Quality of Tilapia by Embrittlement
title_short Regulation of Muscle Quality of Tilapia by Embrittlement
title_sort regulation of muscle quality of tilapia by embrittlement
topic embrittlement
tilapia
muscle quality
regulation
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070129
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AT tingwu regulationofmusclequalityoftilapiabyembrittlement
AT qingxiuwang regulationofmusclequalityoftilapiabyembrittlement
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