Isolation and characterization of a novel lytic bacteriophage Pv27 with biocontrol potential against Vibrio parahaemolyticus infections in shrimp
Background Vibrio parahaemolyticus is a major disease-causing species of Vibrio that is pathogenic to both farmed shrimp and humans. With the increasing spread of antibiotic-resistant V. parahaemolyticus strains, bacteriophages (or phages) are considered potential agents for biocontrol as an alterna...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2025-05-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/19421.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850033887904792576 |
|---|---|
| author | Vu Thi Hien Pham Thi Lanh Thao Thi Phuong Pham Khang Nam Tran Nguyen Dinh Duy Nguyen Thi Hoa Nguyen Xuan Canh Quang Huy Nguyen Seil Kim Dong Van Quyen |
| author_facet | Vu Thi Hien Pham Thi Lanh Thao Thi Phuong Pham Khang Nam Tran Nguyen Dinh Duy Nguyen Thi Hoa Nguyen Xuan Canh Quang Huy Nguyen Seil Kim Dong Van Quyen |
| author_sort | Vu Thi Hien |
| collection | DOAJ |
| description | Background Vibrio parahaemolyticus is a major disease-causing species of Vibrio that is pathogenic to both farmed shrimp and humans. With the increasing spread of antibiotic-resistant V. parahaemolyticus strains, bacteriophages (or phages) are considered potential agents for biocontrol as an alternative to antibiotics. In this study, a bacteriophage capable of lysing V. parahaemolyticus, named Pv27, was isolated, characterized, and evaluated for its potential to control Vibrio infections as a natural therapy. Methods Phage Pv27 was isolated using the double-layer agar technique and its morphology was characterized by transmission electron microscopy (TEM). We further assessed the host range specificity, optimal multiplicity of infection (MOI), one-step growth kinetics, and environmental stability of Pv27 under various pH and temperature conditions. The inhibitory activity of Pv27 against V. parahaemolyticus was evaluated in vitro. Finally, genomic analysis of Pv27 was conducted through whole-genome sequencing, followed by functional annotation of open reading frames (ORFs) and phylogenetic analysis. Results Phage Pv27 exhibited a Myovirus-like morphology, characterized by an icosahedral head (92.7 ± 2 nm) and a contractile tail (103 ± 11 nm), and belongs to the class Caudoviricetes. Pv27 demonstrated high lytic activity against its host cells, with a short latent period of approximately 25 minutes and a large burst size of 112 plaque-forming units (PFU) per infected cell. The phage displayed significant tolerance to a wide pH range (from 3 to 11) and remained heat-stable at temperatures up to 60 °C for 90 min. Genetically, Pv27 possesses a circular double-stranded DNA genome spanning 191,395 base pairs, with a G + C content of 35% and comprising 355 open reading frames (ORFs). Remarkably, up to 23 tRNA genes were identified in its genome, while no genes associated with antibiotic resistance, virulence, or lysogeny were detected, suggesting its potential as a valuable biocontrol agent. Results from the VIRIDIC, Basic Local Alignment Search Tool (BLAST) and phylogenetic analyses revealed that Pv27 is closely related to the two known Vibrio phages, phiKT1024 and phiTY18. Several genes associated with enhanced environmental competitiveness were also identified in the Pv27 genome, including those encoding a PhoH-like phosphate starvation-inducible protein and endolysin. Phage Pv27 effectively lyses V. parahaemolyticus highlighting its potential as a biocontrol agent. |
| format | Article |
| id | doaj-art-8e9ad65e08eb4a51b8b7879029df7a3e |
| institution | DOAJ |
| issn | 2167-8359 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj-art-8e9ad65e08eb4a51b8b7879029df7a3e2025-08-20T02:58:01ZengPeerJ Inc.PeerJ2167-83592025-05-0113e1942110.7717/peerj.19421Isolation and characterization of a novel lytic bacteriophage Pv27 with biocontrol potential against Vibrio parahaemolyticus infections in shrimpVu Thi Hien0Pham Thi Lanh1Thao Thi Phuong Pham2Khang Nam Tran3Nguyen Dinh Duy4Nguyen Thi Hoa5Nguyen Xuan Canh6Quang Huy Nguyen7Seil Kim8Dong Van Quyen9Laboratory of Molecular Microbiology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamLaboratory of Molecular Microbiology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamVinmec Healthcare System, Hanoi, VietnamLaboratory of Molecular Microbiology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamLaboratory of Molecular Microbiology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamLaboratory of Molecular Microbiology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamVietnam National University of Agriculture, Hanoi, VietnamUniversity of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, Hanoi, VietnamUniversity of Science & Technology (UST), Daejeon, Republic of South KoreaLaboratory of Molecular Microbiology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamBackground Vibrio parahaemolyticus is a major disease-causing species of Vibrio that is pathogenic to both farmed shrimp and humans. With the increasing spread of antibiotic-resistant V. parahaemolyticus strains, bacteriophages (or phages) are considered potential agents for biocontrol as an alternative to antibiotics. In this study, a bacteriophage capable of lysing V. parahaemolyticus, named Pv27, was isolated, characterized, and evaluated for its potential to control Vibrio infections as a natural therapy. Methods Phage Pv27 was isolated using the double-layer agar technique and its morphology was characterized by transmission electron microscopy (TEM). We further assessed the host range specificity, optimal multiplicity of infection (MOI), one-step growth kinetics, and environmental stability of Pv27 under various pH and temperature conditions. The inhibitory activity of Pv27 against V. parahaemolyticus was evaluated in vitro. Finally, genomic analysis of Pv27 was conducted through whole-genome sequencing, followed by functional annotation of open reading frames (ORFs) and phylogenetic analysis. Results Phage Pv27 exhibited a Myovirus-like morphology, characterized by an icosahedral head (92.7 ± 2 nm) and a contractile tail (103 ± 11 nm), and belongs to the class Caudoviricetes. Pv27 demonstrated high lytic activity against its host cells, with a short latent period of approximately 25 minutes and a large burst size of 112 plaque-forming units (PFU) per infected cell. The phage displayed significant tolerance to a wide pH range (from 3 to 11) and remained heat-stable at temperatures up to 60 °C for 90 min. Genetically, Pv27 possesses a circular double-stranded DNA genome spanning 191,395 base pairs, with a G + C content of 35% and comprising 355 open reading frames (ORFs). Remarkably, up to 23 tRNA genes were identified in its genome, while no genes associated with antibiotic resistance, virulence, or lysogeny were detected, suggesting its potential as a valuable biocontrol agent. Results from the VIRIDIC, Basic Local Alignment Search Tool (BLAST) and phylogenetic analyses revealed that Pv27 is closely related to the two known Vibrio phages, phiKT1024 and phiTY18. Several genes associated with enhanced environmental competitiveness were also identified in the Pv27 genome, including those encoding a PhoH-like phosphate starvation-inducible protein and endolysin. Phage Pv27 effectively lyses V. parahaemolyticus highlighting its potential as a biocontrol agent.https://peerj.com/articles/19421.pdfVibrio parahaemolyticusShrimpBacteriophagesBiocontrolTransmission electron microscopy (TEM)Host range |
| spellingShingle | Vu Thi Hien Pham Thi Lanh Thao Thi Phuong Pham Khang Nam Tran Nguyen Dinh Duy Nguyen Thi Hoa Nguyen Xuan Canh Quang Huy Nguyen Seil Kim Dong Van Quyen Isolation and characterization of a novel lytic bacteriophage Pv27 with biocontrol potential against Vibrio parahaemolyticus infections in shrimp PeerJ Vibrio parahaemolyticus Shrimp Bacteriophages Biocontrol Transmission electron microscopy (TEM) Host range |
| title | Isolation and characterization of a novel lytic bacteriophage Pv27 with biocontrol potential against Vibrio parahaemolyticus infections in shrimp |
| title_full | Isolation and characterization of a novel lytic bacteriophage Pv27 with biocontrol potential against Vibrio parahaemolyticus infections in shrimp |
| title_fullStr | Isolation and characterization of a novel lytic bacteriophage Pv27 with biocontrol potential against Vibrio parahaemolyticus infections in shrimp |
| title_full_unstemmed | Isolation and characterization of a novel lytic bacteriophage Pv27 with biocontrol potential against Vibrio parahaemolyticus infections in shrimp |
| title_short | Isolation and characterization of a novel lytic bacteriophage Pv27 with biocontrol potential against Vibrio parahaemolyticus infections in shrimp |
| title_sort | isolation and characterization of a novel lytic bacteriophage pv27 with biocontrol potential against vibrio parahaemolyticus infections in shrimp |
| topic | Vibrio parahaemolyticus Shrimp Bacteriophages Biocontrol Transmission electron microscopy (TEM) Host range |
| url | https://peerj.com/articles/19421.pdf |
| work_keys_str_mv | AT vuthihien isolationandcharacterizationofanovellyticbacteriophagepv27withbiocontrolpotentialagainstvibrioparahaemolyticusinfectionsinshrimp AT phamthilanh isolationandcharacterizationofanovellyticbacteriophagepv27withbiocontrolpotentialagainstvibrioparahaemolyticusinfectionsinshrimp AT thaothiphuongpham isolationandcharacterizationofanovellyticbacteriophagepv27withbiocontrolpotentialagainstvibrioparahaemolyticusinfectionsinshrimp AT khangnamtran isolationandcharacterizationofanovellyticbacteriophagepv27withbiocontrolpotentialagainstvibrioparahaemolyticusinfectionsinshrimp AT nguyendinhduy isolationandcharacterizationofanovellyticbacteriophagepv27withbiocontrolpotentialagainstvibrioparahaemolyticusinfectionsinshrimp AT nguyenthihoa isolationandcharacterizationofanovellyticbacteriophagepv27withbiocontrolpotentialagainstvibrioparahaemolyticusinfectionsinshrimp AT nguyenxuancanh isolationandcharacterizationofanovellyticbacteriophagepv27withbiocontrolpotentialagainstvibrioparahaemolyticusinfectionsinshrimp AT quanghuynguyen isolationandcharacterizationofanovellyticbacteriophagepv27withbiocontrolpotentialagainstvibrioparahaemolyticusinfectionsinshrimp AT seilkim isolationandcharacterizationofanovellyticbacteriophagepv27withbiocontrolpotentialagainstvibrioparahaemolyticusinfectionsinshrimp AT dongvanquyen isolationandcharacterizationofanovellyticbacteriophagepv27withbiocontrolpotentialagainstvibrioparahaemolyticusinfectionsinshrimp |