For the question about Molecular Epidemiology of Hepatitis B Virus Infection in the Republic Sakha (Yakutia)
Objective: To estimate the prevalence of genetic variants and features of the molecular epidemiology of HBV in Yakutia residents suffering from HBV.Materials and methods: The study involved 35 patients with chronic hepatitis B from urban and rural areas of Yakutia while most of the group were repres...
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| Format: | Article |
| Language: | Russian |
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Journal Infectology
2016-04-01
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| Series: | Журнал инфектологии |
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| Online Access: | https://journal.niidi.ru/jofin/article/view/463 |
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| author | A. V. Semenov Yu. V. Ostankova V. V. Gerasimova M. A. Bichurina S. L. Mukomolov A. V. Kozlov A. A. Totolian |
| author_facet | A. V. Semenov Yu. V. Ostankova V. V. Gerasimova M. A. Bichurina S. L. Mukomolov A. V. Kozlov A. A. Totolian |
| author_sort | A. V. Semenov |
| collection | DOAJ |
| description | Objective: To estimate the prevalence of genetic variants and features of the molecular epidemiology of HBV in Yakutia residents suffering from HBV.Materials and methods: The study involved 35 patients with chronic hepatitis B from urban and rural areas of Yakutia while most of the group were representatives of the autochthonous population. In the present study we used genotyping by direct sequencing of the Pre-S1 / Pre-S2 / S region of HBV DNA.Results: Based on the phylogenetic analysis of the isolates showed that among patients examined HBV identified only D genotype, which is the most common genotype of HBV in the Russian Federation. It is shown prevalence of HBV subtype D2 (85,8%) compared to the HBV subtype D3 (14,2%).Conclusion: We identified clearly clustered group of HBV isolates and close ties within the group, which suggests the existence of at least four permanent sources of infection, acting for a few years and decades. The systematic application of complex molecular, virological, epidemiological methods and molecular phylogenetics could contribute to the current understanding of the epidemiology of HBV and improve the quality of the traditional methods of supervision in Russia. |
| format | Article |
| id | doaj-art-8e95c0086c244a8685494d58d0a478db |
| institution | Kabale University |
| issn | 2072-6732 |
| language | Russian |
| publishDate | 2016-04-01 |
| publisher | Journal Infectology |
| record_format | Article |
| series | Журнал инфектологии |
| spelling | doaj-art-8e95c0086c244a8685494d58d0a478db2025-08-20T03:44:23ZrusJournal InfectologyЖурнал инфектологии2072-67322016-04-01815765460For the question about Molecular Epidemiology of Hepatitis B Virus Infection in the Republic Sakha (Yakutia)A. V. Semenov0Yu. V. Ostankova1V. V. Gerasimova2M. A. Bichurina3S. L. Mukomolov4A. V. Kozlov5A. A. Totolian6Saint-Petersburg Science Research Institute of Epidemiology and Microbiology named after Pasteur, Saint Petersburg, Russia First Saint-Petersburg State Medical University named after acad. I.P. Pavlov, Saint Petersburg, Russia North-Western State Medical University named after I.I. Mechnikov, Saint Petersburg, RussiaSaint-Petersburg Science Research Institute of Epidemiology and Microbiology named after Pasteur, Saint Petersburg, RussiaNorth-Eastern Federal University named after M.K. Ammosov, Yakutsk, RussiaSaint-Petersburg Science Research Institute of Epidemiology and Microbiology named after Pasteur, Saint Petersburg, RussiaSaint-Petersburg Science Research Institute of Epidemiology and Microbiology named after Pasteur, Saint Petersburg, Russia First Saint-Petersburg State Medical University named after acad. I.P. Pavlov, Saint Petersburg, RussiaNorth-Western State Medical University named after I.I. Mechnikov, Saint Petersburg, RussiaSaint-Petersburg Science Research Institute of Epidemiology and Microbiology named after Pasteur, Saint Petersburg, Russia First Saint-Petersburg State Medical University named after acad. I.P. Pavlov, Saint Petersburg, RussiaObjective: To estimate the prevalence of genetic variants and features of the molecular epidemiology of HBV in Yakutia residents suffering from HBV.Materials and methods: The study involved 35 patients with chronic hepatitis B from urban and rural areas of Yakutia while most of the group were representatives of the autochthonous population. In the present study we used genotyping by direct sequencing of the Pre-S1 / Pre-S2 / S region of HBV DNA.Results: Based on the phylogenetic analysis of the isolates showed that among patients examined HBV identified only D genotype, which is the most common genotype of HBV in the Russian Federation. It is shown prevalence of HBV subtype D2 (85,8%) compared to the HBV subtype D3 (14,2%).Conclusion: We identified clearly clustered group of HBV isolates and close ties within the group, which suggests the existence of at least four permanent sources of infection, acting for a few years and decades. The systematic application of complex molecular, virological, epidemiological methods and molecular phylogenetics could contribute to the current understanding of the epidemiology of HBV and improve the quality of the traditional methods of supervision in Russia.https://journal.niidi.ru/jofin/article/view/463hepatitis bgenotypingsequencinggenotypesubtypemolecular epidemiologyphylogenyrepublic sakhayakutia. |
| spellingShingle | A. V. Semenov Yu. V. Ostankova V. V. Gerasimova M. A. Bichurina S. L. Mukomolov A. V. Kozlov A. A. Totolian For the question about Molecular Epidemiology of Hepatitis B Virus Infection in the Republic Sakha (Yakutia) Журнал инфектологии hepatitis b genotyping sequencing genotype subtype molecular epidemiology phylogeny republic sakha yakutia. |
| title | For the question about Molecular Epidemiology of Hepatitis B Virus Infection in the Republic Sakha (Yakutia) |
| title_full | For the question about Molecular Epidemiology of Hepatitis B Virus Infection in the Republic Sakha (Yakutia) |
| title_fullStr | For the question about Molecular Epidemiology of Hepatitis B Virus Infection in the Republic Sakha (Yakutia) |
| title_full_unstemmed | For the question about Molecular Epidemiology of Hepatitis B Virus Infection in the Republic Sakha (Yakutia) |
| title_short | For the question about Molecular Epidemiology of Hepatitis B Virus Infection in the Republic Sakha (Yakutia) |
| title_sort | for the question about molecular epidemiology of hepatitis b virus infection in the republic sakha yakutia |
| topic | hepatitis b genotyping sequencing genotype subtype molecular epidemiology phylogeny republic sakha yakutia. |
| url | https://journal.niidi.ru/jofin/article/view/463 |
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