Genetic mapping and validation of QTL controlling fruit diameter in cucumber
Abstract Fruit diameter is one of important agronomy traits that has greatly impacts fruit yield and commercial value in cucumber (Cucumis sativus L.). Hence, we preliminary mapping of fruit diameter was conducted to refine its genetic locus. In this study, to genetic mapping of QTLs that control cu...
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BMC
2024-12-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-06000-9 |
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| author | Wenjiao Wang Zhaoying Xu Linna Qian Shuo Hang Yijie Niu Chengcheng Shen Yuping Wei Bin Liu |
| author_facet | Wenjiao Wang Zhaoying Xu Linna Qian Shuo Hang Yijie Niu Chengcheng Shen Yuping Wei Bin Liu |
| author_sort | Wenjiao Wang |
| collection | DOAJ |
| description | Abstract Fruit diameter is one of important agronomy traits that has greatly impacts fruit yield and commercial value in cucumber (Cucumis sativus L.). Hence, we preliminary mapping of fruit diameter was conducted to refine its genetic locus. In this study, to genetic mapping of QTLs that control cucumber fruit diameter, a F2 population with 120 individuals was developed by the East Asian line ‘9930’ (known as narrow fruit diameter) and the European-type cucumber ‘EU224’ (known as wide fruit diameter). Then a Genotyping-by-Sequencing (GBS)-based genetic map with 5662 markers was constructed and the total length is 656.177 cM, with average marker interval of 0.116 cM. Based on this high-density genetic map, a major QTL qfd1.1 related to fruit diameter was detected with a markedly high LOD score 4.07 located approximately 300 kb interval on Chromosome 1 (located between Chr1:1654704–1958556). To confirm qfd1.1 that detected by F2 population, we performed genetic mapping of fruit diameter with an introgression line (IL) about fruit diameter. We developed two KASP markers (FD-1 and FD-2) related to the fruit diameter. Based on this, we inserted the European cucumber EU224 into the qfd1.1 range and targeted widening the fruit diameter of the 9930 cucumber variety, further indicating that qfd1.1 is a new locus regulating the fruit diameter of cucumber. Our findings will support breeders in their research on cucumber fruit diameter. |
| format | Article |
| id | doaj-art-8e8ea2e1172341f8b95fcb93d273f1a4 |
| institution | DOAJ |
| issn | 1471-2229 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj-art-8e8ea2e1172341f8b95fcb93d273f1a42025-08-20T02:43:24ZengBMCBMC Plant Biology1471-22292024-12-0124111010.1186/s12870-024-06000-9Genetic mapping and validation of QTL controlling fruit diameter in cucumberWenjiao Wang0Zhaoying Xu1Linna Qian2Shuo Hang3Yijie Niu4Chengcheng Shen5Yuping Wei6Bin Liu7College of Horticulture, Shanxi Agricultural UniversityCollege of Horticulture, Shanxi Agricultural UniversityCollege of Horticulture, Shanxi Agricultural UniversityCollege of Horticulture, Shanxi Agricultural UniversityCollege of Horticulture, Shanxi Agricultural UniversityCollege of Horticulture, Shanxi Agricultural UniversityCollege of Horticulture, Shanxi Agricultural UniversityHami-melon Research Center, Xinjiang Academy of Agricultural SciencesAbstract Fruit diameter is one of important agronomy traits that has greatly impacts fruit yield and commercial value in cucumber (Cucumis sativus L.). Hence, we preliminary mapping of fruit diameter was conducted to refine its genetic locus. In this study, to genetic mapping of QTLs that control cucumber fruit diameter, a F2 population with 120 individuals was developed by the East Asian line ‘9930’ (known as narrow fruit diameter) and the European-type cucumber ‘EU224’ (known as wide fruit diameter). Then a Genotyping-by-Sequencing (GBS)-based genetic map with 5662 markers was constructed and the total length is 656.177 cM, with average marker interval of 0.116 cM. Based on this high-density genetic map, a major QTL qfd1.1 related to fruit diameter was detected with a markedly high LOD score 4.07 located approximately 300 kb interval on Chromosome 1 (located between Chr1:1654704–1958556). To confirm qfd1.1 that detected by F2 population, we performed genetic mapping of fruit diameter with an introgression line (IL) about fruit diameter. We developed two KASP markers (FD-1 and FD-2) related to the fruit diameter. Based on this, we inserted the European cucumber EU224 into the qfd1.1 range and targeted widening the fruit diameter of the 9930 cucumber variety, further indicating that qfd1.1 is a new locus regulating the fruit diameter of cucumber. Our findings will support breeders in their research on cucumber fruit diameter.https://doi.org/10.1186/s12870-024-06000-9GBSKASPQTL mappingFruit diameterCucumber |
| spellingShingle | Wenjiao Wang Zhaoying Xu Linna Qian Shuo Hang Yijie Niu Chengcheng Shen Yuping Wei Bin Liu Genetic mapping and validation of QTL controlling fruit diameter in cucumber BMC Plant Biology GBS KASP QTL mapping Fruit diameter Cucumber |
| title | Genetic mapping and validation of QTL controlling fruit diameter in cucumber |
| title_full | Genetic mapping and validation of QTL controlling fruit diameter in cucumber |
| title_fullStr | Genetic mapping and validation of QTL controlling fruit diameter in cucumber |
| title_full_unstemmed | Genetic mapping and validation of QTL controlling fruit diameter in cucumber |
| title_short | Genetic mapping and validation of QTL controlling fruit diameter in cucumber |
| title_sort | genetic mapping and validation of qtl controlling fruit diameter in cucumber |
| topic | GBS KASP QTL mapping Fruit diameter Cucumber |
| url | https://doi.org/10.1186/s12870-024-06000-9 |
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