Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells
Abstract Breast cancer remains one of the most lethal diseases for women worldwide. Marine populations are considered a vast reservoir for novel bioactive metabolites, particularly marine Actinomycetes, which are known to produce various bioactive compounds with antitumour, antibacterial, and antifu...
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BMC
2025-06-01
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| Online Access: | https://doi.org/10.1186/s12896-025-00991-5 |
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| author | Abir M. Shata Mokhtar Saeed Rejili Manal M. El-Naggar Samy A. El-Asser Ahmed A. Saleh Elsayed E. Hafez Faouzi Haouala Amany S. Youssef |
| author_facet | Abir M. Shata Mokhtar Saeed Rejili Manal M. El-Naggar Samy A. El-Asser Ahmed A. Saleh Elsayed E. Hafez Faouzi Haouala Amany S. Youssef |
| author_sort | Abir M. Shata |
| collection | DOAJ |
| description | Abstract Breast cancer remains one of the most lethal diseases for women worldwide. Marine populations are considered a vast reservoir for novel bioactive metabolites, particularly marine Actinomycetes, which are known to produce various bioactive compounds with antitumour, antibacterial, and antifungal properties. A promising new marine strain was isolated and identified as Streptomyces albidoflavus strain EgyAB2 (16 S rRNA gene sequence accession number ON680945.1). The anticancer activity of the extracted compounds was tested in the MCF7 cell line using a sulforhodamine B (SRB) bioassay, which revealed an IC50 of 0.36 µg/ml compared to the chemotherapeutic drug 5-fluorouracil (0.35 µg/ml). Additionally, the anticancer activity was confirmed by a dimethyl-thiazol-diphenyl-tetrazolium bromide (MTT) bioassay, which showed an IC50 of 17.46 µg/ml. The mode of action of the treated breast carcinoma (apoptotic effect) was studied via qRT-PCR, revealing a significant role in anticancer treatment. Although the extracted compounds exhibited high antioxidant activity in the diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay, they presented an IC50 of 8.92 µg/ml and an inhibition percentage of 56.08%. Chemical characterisation was performed via GC‒MS, 1 H-NMR, and FTIR spectroscopy analyses, revealing the presence of 2-D N-methyl imidazole, 2-nonadecene, 1-D-2-methyl imidazole, and propane dinitrile, all of which exhibit antitumour activity. |
| format | Article |
| id | doaj-art-8e7a9df2ecd141d9b634c64cf215b4d5 |
| institution | OA Journals |
| issn | 1472-6750 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj-art-8e7a9df2ecd141d9b634c64cf215b4d52025-08-20T02:10:32ZengBMCBMC Biotechnology1472-67502025-06-0125112110.1186/s12896-025-00991-5Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cellsAbir M. Shata0Mokhtar Saeed Rejili1Manal M. El-Naggar2Samy A. El-Asser3Ahmed A. Saleh4Elsayed E. Hafez5Faouzi Haouala6Amany S. Youssef7National Institute of Oceanography and Fisheries, NIOFDepartment of Biology, College of Sciences, Imam Mohammad Ibn Saud Islamic University (IMSIU)National Institute of Oceanography and Fisheries, NIOFFaculty of Science, Department of Botany and Microbiology, Alexandria UniversityCollege of Animal Science & Technology, Yangzhou UniversityDepartment of Plant Protection and Biomolecular Diagnosis, ALCRI City of Scientific Research and Technological ApplicationsDepartment of Biology, College of Sciences, Imam Mohammad Ibn Saud Islamic University (IMSIU)Faculty of Science, Department of Botany and Microbiology, Alexandria UniversityAbstract Breast cancer remains one of the most lethal diseases for women worldwide. Marine populations are considered a vast reservoir for novel bioactive metabolites, particularly marine Actinomycetes, which are known to produce various bioactive compounds with antitumour, antibacterial, and antifungal properties. A promising new marine strain was isolated and identified as Streptomyces albidoflavus strain EgyAB2 (16 S rRNA gene sequence accession number ON680945.1). The anticancer activity of the extracted compounds was tested in the MCF7 cell line using a sulforhodamine B (SRB) bioassay, which revealed an IC50 of 0.36 µg/ml compared to the chemotherapeutic drug 5-fluorouracil (0.35 µg/ml). Additionally, the anticancer activity was confirmed by a dimethyl-thiazol-diphenyl-tetrazolium bromide (MTT) bioassay, which showed an IC50 of 17.46 µg/ml. The mode of action of the treated breast carcinoma (apoptotic effect) was studied via qRT-PCR, revealing a significant role in anticancer treatment. Although the extracted compounds exhibited high antioxidant activity in the diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay, they presented an IC50 of 8.92 µg/ml and an inhibition percentage of 56.08%. Chemical characterisation was performed via GC‒MS, 1 H-NMR, and FTIR spectroscopy analyses, revealing the presence of 2-D N-methyl imidazole, 2-nonadecene, 1-D-2-methyl imidazole, and propane dinitrile, all of which exhibit antitumour activity.https://doi.org/10.1186/s12896-025-00991-5Marine StreptomycesAnti-breast carcinomaMTTbioassaySRBbioassayApoptosis |
| spellingShingle | Abir M. Shata Mokhtar Saeed Rejili Manal M. El-Naggar Samy A. El-Asser Ahmed A. Saleh Elsayed E. Hafez Faouzi Haouala Amany S. Youssef Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells BMC Biotechnology Marine Streptomyces Anti-breast carcinoma MTTbioassay SRBbioassay Apoptosis |
| title | Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells |
| title_full | Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells |
| title_fullStr | Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells |
| title_full_unstemmed | Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells |
| title_short | Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells |
| title_sort | exploring the mechanisms and mode of action of bioactive compounds from marine streptomyces albidoflavus against breast carcinoma cells |
| topic | Marine Streptomyces Anti-breast carcinoma MTTbioassay SRBbioassay Apoptosis |
| url | https://doi.org/10.1186/s12896-025-00991-5 |
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