Comparative Evaluation of Two Venous Sampling Techniques for the Assessment of Pancreatic Insulin and Zinc Release upon Glucose Challenge
Advances in noninvasive imaging modalities have provided opportunities to study β cell function through imaging zinc release from insulin secreting β cells. Understanding the temporal secretory pattern of insulin and zinc corelease after a glucose challenge is essential for proper timing of administ...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2015/789359 |
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| author | Anil Kumar Pillai William Silvers Preston Christensen Matthew Riegel Beverley Adams-Huet Ildiko Lingvay Xiankai Sun Orhan K. Öz |
| author_facet | Anil Kumar Pillai William Silvers Preston Christensen Matthew Riegel Beverley Adams-Huet Ildiko Lingvay Xiankai Sun Orhan K. Öz |
| author_sort | Anil Kumar Pillai |
| collection | DOAJ |
| description | Advances in noninvasive imaging modalities have provided opportunities to study β cell function through imaging zinc release from insulin secreting β cells. Understanding the temporal secretory pattern of insulin and zinc corelease after a glucose challenge is essential for proper timing of administration of zinc sensing probes. Portal venous sampling is an essential part of pharmacological and nutritional studies in animal models. The purpose of this study was to compare two different percutaneous image-guided techniques: transhepatic ultrasound guided portal vein access and transsplenic fluoroscopy guided splenic vein access for ease of access, safety, and evaluation of temporal kinetics of insulin and zinc release into the venous effluent from the pancreas. Both techniques were safe, reproducible, and easy to perform. The mean time required to obtain desired catheter position for venous sampling was 15 minutes shorter using the transsplenic technique. A clear biphasic insulin release profile was observed in both techniques. Statistically higher insulin concentration but similar zinc release after a glucose challenge was observed from splenic vein samples, as compared to the ones from the portal vein. To our knowledge, this is the first report of percutaneous methods to assess zinc release kinetics from the porcine pancreas. |
| format | Article |
| id | doaj-art-8e7a4f921dd14f69b3d8041afbc5cf97 |
| institution | OA Journals |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-8e7a4f921dd14f69b3d8041afbc5cf972025-08-20T02:09:21ZengWileyJournal of Diabetes Research2314-67452314-67532015-01-01201510.1155/2015/789359789359Comparative Evaluation of Two Venous Sampling Techniques for the Assessment of Pancreatic Insulin and Zinc Release upon Glucose ChallengeAnil Kumar Pillai0William Silvers1Preston Christensen2Matthew Riegel3Beverley Adams-Huet4Ildiko Lingvay5Xiankai Sun6Orhan K. Öz7Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USADepartment of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USADepartment of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USAAnimal Resources Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USADepartments of Internal Medicine and Clinical Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USADepartments of Internal Medicine and Clinical Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USADepartment of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USADepartment of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USAAdvances in noninvasive imaging modalities have provided opportunities to study β cell function through imaging zinc release from insulin secreting β cells. Understanding the temporal secretory pattern of insulin and zinc corelease after a glucose challenge is essential for proper timing of administration of zinc sensing probes. Portal venous sampling is an essential part of pharmacological and nutritional studies in animal models. The purpose of this study was to compare two different percutaneous image-guided techniques: transhepatic ultrasound guided portal vein access and transsplenic fluoroscopy guided splenic vein access for ease of access, safety, and evaluation of temporal kinetics of insulin and zinc release into the venous effluent from the pancreas. Both techniques were safe, reproducible, and easy to perform. The mean time required to obtain desired catheter position for venous sampling was 15 minutes shorter using the transsplenic technique. A clear biphasic insulin release profile was observed in both techniques. Statistically higher insulin concentration but similar zinc release after a glucose challenge was observed from splenic vein samples, as compared to the ones from the portal vein. To our knowledge, this is the first report of percutaneous methods to assess zinc release kinetics from the porcine pancreas.http://dx.doi.org/10.1155/2015/789359 |
| spellingShingle | Anil Kumar Pillai William Silvers Preston Christensen Matthew Riegel Beverley Adams-Huet Ildiko Lingvay Xiankai Sun Orhan K. Öz Comparative Evaluation of Two Venous Sampling Techniques for the Assessment of Pancreatic Insulin and Zinc Release upon Glucose Challenge Journal of Diabetes Research |
| title | Comparative Evaluation of Two Venous Sampling Techniques for the Assessment of Pancreatic Insulin and Zinc Release upon Glucose Challenge |
| title_full | Comparative Evaluation of Two Venous Sampling Techniques for the Assessment of Pancreatic Insulin and Zinc Release upon Glucose Challenge |
| title_fullStr | Comparative Evaluation of Two Venous Sampling Techniques for the Assessment of Pancreatic Insulin and Zinc Release upon Glucose Challenge |
| title_full_unstemmed | Comparative Evaluation of Two Venous Sampling Techniques for the Assessment of Pancreatic Insulin and Zinc Release upon Glucose Challenge |
| title_short | Comparative Evaluation of Two Venous Sampling Techniques for the Assessment of Pancreatic Insulin and Zinc Release upon Glucose Challenge |
| title_sort | comparative evaluation of two venous sampling techniques for the assessment of pancreatic insulin and zinc release upon glucose challenge |
| url | http://dx.doi.org/10.1155/2015/789359 |
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