Formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stress
Increasing evidence has demonstrated that oxidative stress impairs oocyte maturation and embryonic development. Conventionally, antioxidants have been applied in vitro systems to improve oocyte maturation and blastocyst rates. Formononetin (FMN) is a flavonoid that has been shown to have various pha...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2024.1520429/full |
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| author | Na Wang Han Yang Yelei Chen Hekun Wang Chaorui Wang Jianglin Fan Yajie Chen Yinghua Li Maobi Zhu |
| author_facet | Na Wang Han Yang Yelei Chen Hekun Wang Chaorui Wang Jianglin Fan Yajie Chen Yinghua Li Maobi Zhu |
| author_sort | Na Wang |
| collection | DOAJ |
| description | Increasing evidence has demonstrated that oxidative stress impairs oocyte maturation and embryonic development. Conventionally, antioxidants have been applied in vitro systems to improve oocyte maturation and blastocyst rates. Formononetin (FMN) is a flavonoid that has been shown to have various pharmacological effects, including antioxidants. In this study, we delved into the impact of FMN, acting as an antioxidant, on the in vitro development of oocytes and blastocysts within the culture system. FMN supplementation at 0.5 μM enhanced the rate of first polar body extrusion and blastocyst formation post parthenogenetic activation. It also increased mitochondrial function and ATP levels, reduced intracellular reactive oxygen species, and elevated intracellular GSH levels in both oocytes and embryos. Moreover, FMN significantly decreased autophagy and apoptosis levels in blastocyst cells, potentially via regulation of the Nrf2/Keap1 pathway. This is the first study to report that FMN supplementation benefits the in vitro culture of oocytes and early embryo development, potentially by regulating oxidative stress, mitochondrial function, and autophagy. |
| format | Article |
| id | doaj-art-8e57656cf53a4b73a7b0ef6ab9a3c475 |
| institution | Kabale University |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-8e57656cf53a4b73a7b0ef6ab9a3c4752025-01-09T06:11:11ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-01-011210.3389/fcell.2024.15204291520429Formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stressNa Wang0Han Yang1Yelei Chen2Hekun Wang3Chaorui Wang4Jianglin Fan5Yajie Chen6Yinghua Li7Maobi Zhu8Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, ChinaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, ChinaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, ChinaDepartment of Gynecology, Jiangmen Maternity and Child Healthcare Hospital, Jiangmen, Guangdong, ChinaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, ChinaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, ChinaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, ChinaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, ChinaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, ChinaIncreasing evidence has demonstrated that oxidative stress impairs oocyte maturation and embryonic development. Conventionally, antioxidants have been applied in vitro systems to improve oocyte maturation and blastocyst rates. Formononetin (FMN) is a flavonoid that has been shown to have various pharmacological effects, including antioxidants. In this study, we delved into the impact of FMN, acting as an antioxidant, on the in vitro development of oocytes and blastocysts within the culture system. FMN supplementation at 0.5 μM enhanced the rate of first polar body extrusion and blastocyst formation post parthenogenetic activation. It also increased mitochondrial function and ATP levels, reduced intracellular reactive oxygen species, and elevated intracellular GSH levels in both oocytes and embryos. Moreover, FMN significantly decreased autophagy and apoptosis levels in blastocyst cells, potentially via regulation of the Nrf2/Keap1 pathway. This is the first study to report that FMN supplementation benefits the in vitro culture of oocytes and early embryo development, potentially by regulating oxidative stress, mitochondrial function, and autophagy.https://www.frontiersin.org/articles/10.3389/fcell.2024.1520429/fullformononetinmitochondrial functionoxidative stressNrf2/Keap1autophagy |
| spellingShingle | Na Wang Han Yang Yelei Chen Hekun Wang Chaorui Wang Jianglin Fan Yajie Chen Yinghua Li Maobi Zhu Formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stress Frontiers in Cell and Developmental Biology formononetin mitochondrial function oxidative stress Nrf2/Keap1 autophagy |
| title | Formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stress |
| title_full | Formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stress |
| title_fullStr | Formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stress |
| title_full_unstemmed | Formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stress |
| title_short | Formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stress |
| title_sort | formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stress |
| topic | formononetin mitochondrial function oxidative stress Nrf2/Keap1 autophagy |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2024.1520429/full |
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