COMPARISON OF AEROKEYII SCHEME AND API20E SYSTEM FOR IDENTIFICATION OF AEROMONAS HYDROPHILA ISOLATED FROM WELLS WATER IN THIQAR PROVINCE-IRAQ
Untreated water is well-known source of A.hydrophila which is in addition to its enteropathogenicpotential ,also display resistance to commonly used antibiotics , so this study aimed to compare AerokeyII and API20E in identification of A.hydrophila with studying of some virulence factors andantibiog...
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College of Veterinary Medicine, University of Basrah, Iraq
2016-11-01
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| Series: | Basrah Journal of Veterinary Research |
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| Online Access: | https://bjvr.uobasrah.edu.iq/article_172794_adc9b77a27ad34e277ad8cbf72e9d557.pdf |
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| author | Muslim Dhaher Musa Waffa Abdulelah Ahmed |
| author_facet | Muslim Dhaher Musa Waffa Abdulelah Ahmed |
| author_sort | Muslim Dhaher Musa |
| collection | DOAJ |
| description | Untreated water is well-known source of A.hydrophila which is in addition to its enteropathogenicpotential ,also display resistance to commonly used antibiotics , so this study aimed to compare AerokeyII and API20E in identification of A.hydrophila with studying of some virulence factors andantibiogram profile in untreated wells water in Thi-Qar province. Isolation was conducted by employingAmpicilin Blood Agar (ABA30) and MacConkey agar medium, the suspected colonies were identifiedby using biochemical scheme (AerokeyII) , and API20E . The results of this study revealed thatA.hydrophila was recovered from 8 out of 30 wells with incidence rate (27.6%), incidence variation wasnoted among different regions which was statistically significant . All isolates showed β-hemolysis ofhuman erythrocytes , 75% have proteolytic activity and 50% of isolates were DNase positive. Resultsof Antibiogram analysis revealed that all isolates exhibit resistance in percentage (100%) to fiveantibiotics including ;Clindamycin , Cephalothin , Vancomycin , ticarcilin-clavulanic acid , Ceftazidime,and the resistance to Cefoxitin was 75%, while all isolates were (100%) susceptible to Gentamicin ,Amikacin, Chloramphenicol, Ofloxacin ,Ciprofloxacin, Naldxic acid, Imipenem , Norfloxacin, andDeoxycyclin. The susceptibility to Ceftriaxone was 62.5% , Streptomycin 87.5% and Trimethoprime87.5% . The study concluded that , high correlation between Aerokey II and API20E in identification ofA.hydrophila and untreated wells water are an important source of multi-drug resistant enteropathogenicA.hydrophila which pose public health threat especially to individuals using this kind of water source |
| format | Article |
| id | doaj-art-8e4e5a17888843839b995adf4f557c5f |
| institution | DOAJ |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2016-11-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
| record_format | Article |
| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-8e4e5a17888843839b995adf4f557c5f2025-08-20T03:09:58ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562016-11-01153 (Proceeding of 5th International Scientific Conference, College of Veterinary Medicine University of Basrah, Iraq)16718210.23975/bjvetr.2016.172794172794COMPARISON OF AEROKEYII SCHEME AND API20E SYSTEM FOR IDENTIFICATION OF AEROMONAS HYDROPHILA ISOLATED FROM WELLS WATER IN THIQAR PROVINCE-IRAQMuslim Dhaher Musa0Waffa Abdulelah Ahmed1Department of Nursing , Technical Institute , AL-Nasiryah , Southern Technical University, Thi-Qar , IraqDepartment of Microbiology , College of Veterinary Medicine , University of Baghdad, Baghdad , IraqUntreated water is well-known source of A.hydrophila which is in addition to its enteropathogenicpotential ,also display resistance to commonly used antibiotics , so this study aimed to compare AerokeyII and API20E in identification of A.hydrophila with studying of some virulence factors andantibiogram profile in untreated wells water in Thi-Qar province. Isolation was conducted by employingAmpicilin Blood Agar (ABA30) and MacConkey agar medium, the suspected colonies were identifiedby using biochemical scheme (AerokeyII) , and API20E . The results of this study revealed thatA.hydrophila was recovered from 8 out of 30 wells with incidence rate (27.6%), incidence variation wasnoted among different regions which was statistically significant . All isolates showed β-hemolysis ofhuman erythrocytes , 75% have proteolytic activity and 50% of isolates were DNase positive. Resultsof Antibiogram analysis revealed that all isolates exhibit resistance in percentage (100%) to fiveantibiotics including ;Clindamycin , Cephalothin , Vancomycin , ticarcilin-clavulanic acid , Ceftazidime,and the resistance to Cefoxitin was 75%, while all isolates were (100%) susceptible to Gentamicin ,Amikacin, Chloramphenicol, Ofloxacin ,Ciprofloxacin, Naldxic acid, Imipenem , Norfloxacin, andDeoxycyclin. The susceptibility to Ceftriaxone was 62.5% , Streptomycin 87.5% and Trimethoprime87.5% . The study concluded that , high correlation between Aerokey II and API20E in identification ofA.hydrophila and untreated wells water are an important source of multi-drug resistant enteropathogenicA.hydrophila which pose public health threat especially to individuals using this kind of water sourcehttps://bjvr.uobasrah.edu.iq/article_172794_adc9b77a27ad34e277ad8cbf72e9d557.pdfa.hydrophilawellsaerokeyii |
| spellingShingle | Muslim Dhaher Musa Waffa Abdulelah Ahmed COMPARISON OF AEROKEYII SCHEME AND API20E SYSTEM FOR IDENTIFICATION OF AEROMONAS HYDROPHILA ISOLATED FROM WELLS WATER IN THIQAR PROVINCE-IRAQ Basrah Journal of Veterinary Research a.hydrophila wells aerokeyii |
| title | COMPARISON OF AEROKEYII SCHEME AND API20E SYSTEM FOR IDENTIFICATION OF AEROMONAS HYDROPHILA ISOLATED FROM WELLS WATER IN THIQAR PROVINCE-IRAQ |
| title_full | COMPARISON OF AEROKEYII SCHEME AND API20E SYSTEM FOR IDENTIFICATION OF AEROMONAS HYDROPHILA ISOLATED FROM WELLS WATER IN THIQAR PROVINCE-IRAQ |
| title_fullStr | COMPARISON OF AEROKEYII SCHEME AND API20E SYSTEM FOR IDENTIFICATION OF AEROMONAS HYDROPHILA ISOLATED FROM WELLS WATER IN THIQAR PROVINCE-IRAQ |
| title_full_unstemmed | COMPARISON OF AEROKEYII SCHEME AND API20E SYSTEM FOR IDENTIFICATION OF AEROMONAS HYDROPHILA ISOLATED FROM WELLS WATER IN THIQAR PROVINCE-IRAQ |
| title_short | COMPARISON OF AEROKEYII SCHEME AND API20E SYSTEM FOR IDENTIFICATION OF AEROMONAS HYDROPHILA ISOLATED FROM WELLS WATER IN THIQAR PROVINCE-IRAQ |
| title_sort | comparison of aerokeyii scheme and api20e system for identification of aeromonas hydrophila isolated from wells water in thiqar province iraq |
| topic | a.hydrophila wells aerokeyii |
| url | https://bjvr.uobasrah.edu.iq/article_172794_adc9b77a27ad34e277ad8cbf72e9d557.pdf |
| work_keys_str_mv | AT muslimdhahermusa comparisonofaerokeyiischemeandapi20esystemforidentificationofaeromonashydrophilaisolatedfromwellswaterinthiqarprovinceiraq AT waffaabdulelahahmed comparisonofaerokeyiischemeandapi20esystemforidentificationofaeromonashydrophilaisolatedfromwellswaterinthiqarprovinceiraq |