Immunopeptidomics of <i>Salmonella enterica</i> Serovar <i>Typhimurium</i>-Infected Pig Macrophages Genotyped for Class II Molecules
Salmonellosis is a zoonotic infection that has a major impact on human health; consuming contaminated pork products is the main source of such infection. Vaccination responses to classic vaccines have been unsatisfactory; that is why peptide subunit-based vaccines represent an excellent alternative....
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2024-10-01
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| author | Carmen Celis-Giraldo Carlos F. Suárez William Agudelo Nieves Ibarrola Rosa Degano Jaime Díaz Raúl Manzano-Román Manuel A. Patarroyo |
| author_facet | Carmen Celis-Giraldo Carlos F. Suárez William Agudelo Nieves Ibarrola Rosa Degano Jaime Díaz Raúl Manzano-Román Manuel A. Patarroyo |
| author_sort | Carmen Celis-Giraldo |
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| description | Salmonellosis is a zoonotic infection that has a major impact on human health; consuming contaminated pork products is the main source of such infection. Vaccination responses to classic vaccines have been unsatisfactory; that is why peptide subunit-based vaccines represent an excellent alternative. Immunopeptidomics was used in this study as a novel approach for identifying antigens coupled to major histocompatibility complex class II molecules. Three homozygous individuals having three different haplotypes (Lr-0.23, Lr-0.12, and Lr-0.21) were thus selected as donors; peripheral blood macrophages were then obtained and stimulated with <i>Salmonella typhimurium</i> (MOI 1:40). Although similarities were observed regarding peptide length distribution, elution patterns varied between individuals; in total, 1990 unique peptides were identified as follows: 372 for Pig 1 (Lr-0.23), 438 for Pig 2 (Lr.0.12) and 1180 for Pig 3 (Lr.0.21). Thirty-one <i>S. typhimurium</i> unique peptides were identified; most of the identified peptides belonged to outer membrane protein A and chaperonin GroEL. Notably, 87% of the identified bacterial peptides were predicted in silico to be elution ligands. These results encourage further in vivo studies to assess the immunogenicity of the identified peptides, as well as their usefulness as possible protective vaccine candidates. |
| format | Article |
| id | doaj-art-8e4899762e084c3cb9b8ded830b300ca |
| institution | OA Journals |
| issn | 2079-7737 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-8e4899762e084c3cb9b8ded830b300ca2025-08-20T02:10:57ZengMDPI AGBiology2079-77372024-10-01131083210.3390/biology13100832Immunopeptidomics of <i>Salmonella enterica</i> Serovar <i>Typhimurium</i>-Infected Pig Macrophages Genotyped for Class II MoleculesCarmen Celis-Giraldo0Carlos F. Suárez1William Agudelo2Nieves Ibarrola3Rosa Degano4Jaime Díaz5Raúl Manzano-Román6Manuel A. Patarroyo7Veterinary Medicine Programme, Universidad de Ciencias Aplicadas y Ambientales (U.D.C.A), Bogotá 111166, ColombiaGrupo de Investigación Básica en Biología Molecular e Inmunología (GIBBMI), Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá 111321, ColombiaGrupo de Investigación Básica en Biología Molecular e Inmunología (GIBBMI), Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá 111321, ColombiaCentro de Investigación del Cáncer e Instituto de Biología Molecular y Celular del Cáncer (IBMCC), CSIC-Universidad de Salamanca, 37007 Salamanca, SpainCentro de Investigación del Cáncer e Instituto de Biología Molecular y Celular del Cáncer (IBMCC), CSIC-Universidad de Salamanca, 37007 Salamanca, SpainVeterinary Medicine Programme, Universidad de Ciencias Aplicadas y Ambientales (U.D.C.A), Bogotá 111166, ColombiaInfectious and Tropical Diseases Group (e-INTRO), IBSAL-CIETUS (Instituto de Investigación Biomédica de Salamanca—Centro de Investigación de Enfermedades Tropicales de la Universidad de Salamanca), Pharmacy Faculty, Universidad de Salamanca, 37007 Salamanca, SpainGrupo de Investigación Básica en Biología Molecular e Inmunología (GIBBMI), Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá 111321, ColombiaSalmonellosis is a zoonotic infection that has a major impact on human health; consuming contaminated pork products is the main source of such infection. Vaccination responses to classic vaccines have been unsatisfactory; that is why peptide subunit-based vaccines represent an excellent alternative. Immunopeptidomics was used in this study as a novel approach for identifying antigens coupled to major histocompatibility complex class II molecules. Three homozygous individuals having three different haplotypes (Lr-0.23, Lr-0.12, and Lr-0.21) were thus selected as donors; peripheral blood macrophages were then obtained and stimulated with <i>Salmonella typhimurium</i> (MOI 1:40). Although similarities were observed regarding peptide length distribution, elution patterns varied between individuals; in total, 1990 unique peptides were identified as follows: 372 for Pig 1 (Lr-0.23), 438 for Pig 2 (Lr.0.12) and 1180 for Pig 3 (Lr.0.21). Thirty-one <i>S. typhimurium</i> unique peptides were identified; most of the identified peptides belonged to outer membrane protein A and chaperonin GroEL. Notably, 87% of the identified bacterial peptides were predicted in silico to be elution ligands. These results encourage further in vivo studies to assess the immunogenicity of the identified peptides, as well as their usefulness as possible protective vaccine candidates.https://www.mdpi.com/2079-7737/13/10/832<i>Salmonella typhimurium</i>immunopeptidomicspeptidevaccinepig |
| spellingShingle | Carmen Celis-Giraldo Carlos F. Suárez William Agudelo Nieves Ibarrola Rosa Degano Jaime Díaz Raúl Manzano-Román Manuel A. Patarroyo Immunopeptidomics of <i>Salmonella enterica</i> Serovar <i>Typhimurium</i>-Infected Pig Macrophages Genotyped for Class II Molecules Biology <i>Salmonella typhimurium</i> immunopeptidomics peptide vaccine pig |
| title | Immunopeptidomics of <i>Salmonella enterica</i> Serovar <i>Typhimurium</i>-Infected Pig Macrophages Genotyped for Class II Molecules |
| title_full | Immunopeptidomics of <i>Salmonella enterica</i> Serovar <i>Typhimurium</i>-Infected Pig Macrophages Genotyped for Class II Molecules |
| title_fullStr | Immunopeptidomics of <i>Salmonella enterica</i> Serovar <i>Typhimurium</i>-Infected Pig Macrophages Genotyped for Class II Molecules |
| title_full_unstemmed | Immunopeptidomics of <i>Salmonella enterica</i> Serovar <i>Typhimurium</i>-Infected Pig Macrophages Genotyped for Class II Molecules |
| title_short | Immunopeptidomics of <i>Salmonella enterica</i> Serovar <i>Typhimurium</i>-Infected Pig Macrophages Genotyped for Class II Molecules |
| title_sort | immunopeptidomics of i salmonella enterica i serovar i typhimurium i infected pig macrophages genotyped for class ii molecules |
| topic | <i>Salmonella typhimurium</i> immunopeptidomics peptide vaccine pig |
| url | https://www.mdpi.com/2079-7737/13/10/832 |
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