Temporary Immersion Bioreactor for In Vitro Multiplication of Raspberry (<i>Rubus idaeus</i> L.)
Raspberry (<i>Rubus idaeus</i> L.) micropropagation is an alternative for obtaining plantlets with high genetic and phytosanitary quality. The objective of this study was to establish a protocol for the micropropagation of raspberry (<i>Rubus idaeus</i> L.) using the temporar...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/11/7/842 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850077334054371328 |
|---|---|
| author | Bruno Reyes-Beristain Eucario Mancilla-Álvarez José Abel López-Buenfil Jericó Jabín Bello-Bello |
| author_facet | Bruno Reyes-Beristain Eucario Mancilla-Álvarez José Abel López-Buenfil Jericó Jabín Bello-Bello |
| author_sort | Bruno Reyes-Beristain |
| collection | DOAJ |
| description | Raspberry (<i>Rubus idaeus</i> L.) micropropagation is an alternative for obtaining plantlets with high genetic and phytosanitary quality. The objective of this study was to establish a protocol for the micropropagation of raspberry (<i>Rubus idaeus</i> L.) using the temporary immersion bioreactor, under intermittent immersion periods and different culture medium volumes. The effect of the liquid medium using the TIB and semisolid was evaluated. Different immersion frequencies and culture medium volumes per explant were evaluated in the TIB. In all treatments, the number of shoots per explant, shoot length, number of leaves per explant, percentage of hyperhydricity, and chlorophyll and β-carotene content at multiplication stage were evaluated. The generated shoots, without a root system, were transferred to the acclimatization stage. The results show that the TIB with an immersion frequency of 2 min every 8 h and a volume of 25 mL of culture medium per explant had the best developmental parameters, with 5.75 shoots per explant, a shoot length of 3.44 cm, and 2% hyperhydricity. The highest chlorophyll and β-carotene content was observed in the TIB at different immersion frequencies of 4, 8 and 12 h, with 25 and 50 mL per explant. Survival percentages higher than 96% were observed in all methods evaluated. In conclusion, the evaluated immersion system is an efficient alternative for <i>R. idaeus</i> micropropagation, without using a rooting stage. |
| format | Article |
| id | doaj-art-8e482aea95fd4773b8e9bfab9e769c1c |
| institution | DOAJ |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-8e482aea95fd4773b8e9bfab9e769c1c2025-08-20T02:45:49ZengMDPI AGHorticulturae2311-75242025-07-0111784210.3390/horticulturae11070842Temporary Immersion Bioreactor for In Vitro Multiplication of Raspberry (<i>Rubus idaeus</i> L.)Bruno Reyes-Beristain0Eucario Mancilla-Álvarez1José Abel López-Buenfil2Jericó Jabín Bello-Bello3Postgraduate College-Campus Cordoba, Amatlan de los Reyes, Veracruz 94953, MexicoPostgraduate College-Campus Cordoba, Amatlan de los Reyes, Veracruz 94953, MexicoPostgraduate College-Campus Montecillo, Texcoco 56264, State of Mexico, MexicoSECIHTI-Postgraduate College-Campus Cordoba, Amatlan de los Reyes, Veracruz 94953, MexicoRaspberry (<i>Rubus idaeus</i> L.) micropropagation is an alternative for obtaining plantlets with high genetic and phytosanitary quality. The objective of this study was to establish a protocol for the micropropagation of raspberry (<i>Rubus idaeus</i> L.) using the temporary immersion bioreactor, under intermittent immersion periods and different culture medium volumes. The effect of the liquid medium using the TIB and semisolid was evaluated. Different immersion frequencies and culture medium volumes per explant were evaluated in the TIB. In all treatments, the number of shoots per explant, shoot length, number of leaves per explant, percentage of hyperhydricity, and chlorophyll and β-carotene content at multiplication stage were evaluated. The generated shoots, without a root system, were transferred to the acclimatization stage. The results show that the TIB with an immersion frequency of 2 min every 8 h and a volume of 25 mL of culture medium per explant had the best developmental parameters, with 5.75 shoots per explant, a shoot length of 3.44 cm, and 2% hyperhydricity. The highest chlorophyll and β-carotene content was observed in the TIB at different immersion frequencies of 4, 8 and 12 h, with 25 and 50 mL per explant. Survival percentages higher than 96% were observed in all methods evaluated. In conclusion, the evaluated immersion system is an efficient alternative for <i>R. idaeus</i> micropropagation, without using a rooting stage.https://www.mdpi.com/2311-7524/11/7/842immersion frequencyculture volumerootingmicropropagationacclimatization |
| spellingShingle | Bruno Reyes-Beristain Eucario Mancilla-Álvarez José Abel López-Buenfil Jericó Jabín Bello-Bello Temporary Immersion Bioreactor for In Vitro Multiplication of Raspberry (<i>Rubus idaeus</i> L.) Horticulturae immersion frequency culture volume rooting micropropagation acclimatization |
| title | Temporary Immersion Bioreactor for In Vitro Multiplication of Raspberry (<i>Rubus idaeus</i> L.) |
| title_full | Temporary Immersion Bioreactor for In Vitro Multiplication of Raspberry (<i>Rubus idaeus</i> L.) |
| title_fullStr | Temporary Immersion Bioreactor for In Vitro Multiplication of Raspberry (<i>Rubus idaeus</i> L.) |
| title_full_unstemmed | Temporary Immersion Bioreactor for In Vitro Multiplication of Raspberry (<i>Rubus idaeus</i> L.) |
| title_short | Temporary Immersion Bioreactor for In Vitro Multiplication of Raspberry (<i>Rubus idaeus</i> L.) |
| title_sort | temporary immersion bioreactor for in vitro multiplication of raspberry i rubus idaeus i l |
| topic | immersion frequency culture volume rooting micropropagation acclimatization |
| url | https://www.mdpi.com/2311-7524/11/7/842 |
| work_keys_str_mv | AT brunoreyesberistain temporaryimmersionbioreactorforinvitromultiplicationofraspberryirubusidaeusil AT eucariomancillaalvarez temporaryimmersionbioreactorforinvitromultiplicationofraspberryirubusidaeusil AT joseabellopezbuenfil temporaryimmersionbioreactorforinvitromultiplicationofraspberryirubusidaeusil AT jericojabinbellobello temporaryimmersionbioreactorforinvitromultiplicationofraspberryirubusidaeusil |