MatE transporter affects methane metabolism in Methermicoccus shengliensis and is modulated by methoxylated aromatic compounds
Abstract Methoxylated aromatic compounds, are abundant in subsurface ecosystems. Recently, it was discovered that Methermicoccus shengliensis can convert methoxylated aromatics to methane. Specifically, the MATE family transporters (MatE) and transduction-like protein (Tlp) were hypothesized to play...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-02-01
|
| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07583-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823861741800390656 |
|---|---|
| author | Huan Leng Dong Wang Qing Yang Shuxin Wang Leizhou Guo Pengyan Zhao Yi Chen Lirong Dai Guihong Cha Liping Bai Frank Delvigne |
| author_facet | Huan Leng Dong Wang Qing Yang Shuxin Wang Leizhou Guo Pengyan Zhao Yi Chen Lirong Dai Guihong Cha Liping Bai Frank Delvigne |
| author_sort | Huan Leng |
| collection | DOAJ |
| description | Abstract Methoxylated aromatic compounds, are abundant in subsurface ecosystems. Recently, it was discovered that Methermicoccus shengliensis can convert methoxylated aromatics to methane. Specifically, the MATE family transporters (MatE) and transduction-like protein (Tlp) were hypothesized to play a crucial role in substrate transport. However, their biological function and the transporting model remained unclear. To address this knowledge gap, we employed bacterial two-hybrid and structural model assays to investigate the interaction between Tlp and MatE. Our results revealed that Tlp senses 2-methoxybenzoate and interacts with MatE to facilitate substrate transport. Furthermore, we observed that the matE knock-out mutant significantly impaired the growth and methane production of M. shengliensis when using 2-methoxybenzoate as a substrate, highlighting the essential role of MatE in methoxydotrophic methanogenesis. Overall, our findings suggest that the MatE-Tlp system regulates substrate uptake and methane metabolism in M. shengliensis, providing new avenues for reducing global methane emissions caused by methanogens. |
| format | Article |
| id | doaj-art-8e3e37a75b1146b28c7d90c08a5b8600 |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-8e3e37a75b1146b28c7d90c08a5b86002025-02-09T12:50:40ZengNature PortfolioCommunications Biology2399-36422025-02-01811710.1038/s42003-025-07583-1MatE transporter affects methane metabolism in Methermicoccus shengliensis and is modulated by methoxylated aromatic compoundsHuan Leng0Dong Wang1Qing Yang2Shuxin Wang3Leizhou Guo4Pengyan Zhao5Yi Chen6Lirong Dai7Guihong Cha8Liping Bai9Frank Delvigne10Key Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture and Rural AffairsCollege of Veterinary Medicine, Northwest A&F UniversityKey Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture and Rural AffairsKey Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture and Rural AffairsKey Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture and Rural AffairsKey Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture and Rural AffairsKey Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture and Rural AffairsKey Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture and Rural AffairsKey Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture and Rural AffairsKey Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture and Rural AffairsTerra Research and Teaching Centre, Microbial Processes and Interactions (MiPI), Gembloux Agro-Bio Tech, University of LiègeAbstract Methoxylated aromatic compounds, are abundant in subsurface ecosystems. Recently, it was discovered that Methermicoccus shengliensis can convert methoxylated aromatics to methane. Specifically, the MATE family transporters (MatE) and transduction-like protein (Tlp) were hypothesized to play a crucial role in substrate transport. However, their biological function and the transporting model remained unclear. To address this knowledge gap, we employed bacterial two-hybrid and structural model assays to investigate the interaction between Tlp and MatE. Our results revealed that Tlp senses 2-methoxybenzoate and interacts with MatE to facilitate substrate transport. Furthermore, we observed that the matE knock-out mutant significantly impaired the growth and methane production of M. shengliensis when using 2-methoxybenzoate as a substrate, highlighting the essential role of MatE in methoxydotrophic methanogenesis. Overall, our findings suggest that the MatE-Tlp system regulates substrate uptake and methane metabolism in M. shengliensis, providing new avenues for reducing global methane emissions caused by methanogens.https://doi.org/10.1038/s42003-025-07583-1 |
| spellingShingle | Huan Leng Dong Wang Qing Yang Shuxin Wang Leizhou Guo Pengyan Zhao Yi Chen Lirong Dai Guihong Cha Liping Bai Frank Delvigne MatE transporter affects methane metabolism in Methermicoccus shengliensis and is modulated by methoxylated aromatic compounds Communications Biology |
| title | MatE transporter affects methane metabolism in Methermicoccus shengliensis and is modulated by methoxylated aromatic compounds |
| title_full | MatE transporter affects methane metabolism in Methermicoccus shengliensis and is modulated by methoxylated aromatic compounds |
| title_fullStr | MatE transporter affects methane metabolism in Methermicoccus shengliensis and is modulated by methoxylated aromatic compounds |
| title_full_unstemmed | MatE transporter affects methane metabolism in Methermicoccus shengliensis and is modulated by methoxylated aromatic compounds |
| title_short | MatE transporter affects methane metabolism in Methermicoccus shengliensis and is modulated by methoxylated aromatic compounds |
| title_sort | mate transporter affects methane metabolism in methermicoccus shengliensis and is modulated by methoxylated aromatic compounds |
| url | https://doi.org/10.1038/s42003-025-07583-1 |
| work_keys_str_mv | AT huanleng matetransporteraffectsmethanemetabolisminmethermicoccusshengliensisandismodulatedbymethoxylatedaromaticcompounds AT dongwang matetransporteraffectsmethanemetabolisminmethermicoccusshengliensisandismodulatedbymethoxylatedaromaticcompounds AT qingyang matetransporteraffectsmethanemetabolisminmethermicoccusshengliensisandismodulatedbymethoxylatedaromaticcompounds AT shuxinwang matetransporteraffectsmethanemetabolisminmethermicoccusshengliensisandismodulatedbymethoxylatedaromaticcompounds AT leizhouguo matetransporteraffectsmethanemetabolisminmethermicoccusshengliensisandismodulatedbymethoxylatedaromaticcompounds AT pengyanzhao matetransporteraffectsmethanemetabolisminmethermicoccusshengliensisandismodulatedbymethoxylatedaromaticcompounds AT yichen matetransporteraffectsmethanemetabolisminmethermicoccusshengliensisandismodulatedbymethoxylatedaromaticcompounds AT lirongdai matetransporteraffectsmethanemetabolisminmethermicoccusshengliensisandismodulatedbymethoxylatedaromaticcompounds AT guihongcha matetransporteraffectsmethanemetabolisminmethermicoccusshengliensisandismodulatedbymethoxylatedaromaticcompounds AT lipingbai matetransporteraffectsmethanemetabolisminmethermicoccusshengliensisandismodulatedbymethoxylatedaromaticcompounds AT frankdelvigne matetransporteraffectsmethanemetabolisminmethermicoccusshengliensisandismodulatedbymethoxylatedaromaticcompounds |