The cellular response of lipopolysaccharide-induced inflammation in keratoconus human corneal fibroblasts to RB-PDT: Insights into cytokines, chemokines and related signaling pathways.
<h4>Purpose</h4>Rose Bengal Photodynamic Therapy (RB-PDT) offers dual therapeutic benefits by enhancing corneal stiffness and providing antibacterial activity, presenting significant potential for patients with keratoconus complicated by keratitis. Our purpose was to assess the effect of...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0318132 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832540196709597184 |
|---|---|
| author | Ning Chai Tanja Stachon Sabrina Häcker Tim Berger Zhen Li Maryam Amini Shweta Suiwal Berthold Seitz Achim Langenbucher Nóra Szentmáry |
| author_facet | Ning Chai Tanja Stachon Sabrina Häcker Tim Berger Zhen Li Maryam Amini Shweta Suiwal Berthold Seitz Achim Langenbucher Nóra Szentmáry |
| author_sort | Ning Chai |
| collection | DOAJ |
| description | <h4>Purpose</h4>Rose Bengal Photodynamic Therapy (RB-PDT) offers dual therapeutic benefits by enhancing corneal stiffness and providing antibacterial activity, presenting significant potential for patients with keratoconus complicated by keratitis. Our purpose was to assess the effect of rose bengal photodynamic therapy (RB-PDT) on the expression of pro-inflammatory cytokines and chemokines, as well as on extracellular matrix (ECM)-related molecules, in lipopolysaccharide (LPS)-induced inflammation of keratoconus human corneal fibroblasts (KC-HCFs). Additionally, the involvement of the mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling pathways which are downstream of the Toll-like receptor 4 (TLR4) pathway were examined.<h4>Methods</h4>KC-HCFs were stimulated with varying concentrations of LPS (0-10 μg/ml), which was followed by RB-PDT. The expression levels of interleukin-1β (IL-1β), IL-6, IL-8, interferon alpha 2 (IFNA2), IFNB1, intercellular adhesion molecule 1 (ICAM-1), chemokine (C-C motif) ligand 4 (CCL-4), collagen I, collagen V, lysyl oxidase (LOX), transforming growth factor β 1(TGF-β1) were measured using qPCR, ELISA, or western blot. The activation of the NF-κB and MAPK pathways was assessed using qPCR and western blot.<h4>Results</h4>In LPS-induced inflammation of KC-HCFs, the expression of IL-6 was further amplified by the treatment with RB-PDT (p = 0.001). However, the activation of the MAPK and NF-κB pathways did not increase following RB-PDT. Additionally, RB-PDT reduced the transcription of collagen I and collagen V (p≤0.03), while the transcription of LOX and TGF-β1 secretion remained unchanged in KC-HCFs exposed to LPS.<h4>Conclusion</h4>In LPS-induced inflammation of KC-HCFs treated with RB-PDT, despite the increased expression of pro-inflammatory cytokines, the activation of the TLR4 signaling pathways is lacking. RB-PDT may have no adverse effects on corneal scar formation of keratoconus corneas in the short term. |
| format | Article |
| id | doaj-art-8e30b0e66949463b98827a025de171a9 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-8e30b0e66949463b98827a025de171a92025-02-05T05:32:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01201e031813210.1371/journal.pone.0318132The cellular response of lipopolysaccharide-induced inflammation in keratoconus human corneal fibroblasts to RB-PDT: Insights into cytokines, chemokines and related signaling pathways.Ning ChaiTanja StachonSabrina HäckerTim BergerZhen LiMaryam AminiShweta SuiwalBerthold SeitzAchim LangenbucherNóra Szentmáry<h4>Purpose</h4>Rose Bengal Photodynamic Therapy (RB-PDT) offers dual therapeutic benefits by enhancing corneal stiffness and providing antibacterial activity, presenting significant potential for patients with keratoconus complicated by keratitis. Our purpose was to assess the effect of rose bengal photodynamic therapy (RB-PDT) on the expression of pro-inflammatory cytokines and chemokines, as well as on extracellular matrix (ECM)-related molecules, in lipopolysaccharide (LPS)-induced inflammation of keratoconus human corneal fibroblasts (KC-HCFs). Additionally, the involvement of the mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling pathways which are downstream of the Toll-like receptor 4 (TLR4) pathway were examined.<h4>Methods</h4>KC-HCFs were stimulated with varying concentrations of LPS (0-10 μg/ml), which was followed by RB-PDT. The expression levels of interleukin-1β (IL-1β), IL-6, IL-8, interferon alpha 2 (IFNA2), IFNB1, intercellular adhesion molecule 1 (ICAM-1), chemokine (C-C motif) ligand 4 (CCL-4), collagen I, collagen V, lysyl oxidase (LOX), transforming growth factor β 1(TGF-β1) were measured using qPCR, ELISA, or western blot. The activation of the NF-κB and MAPK pathways was assessed using qPCR and western blot.<h4>Results</h4>In LPS-induced inflammation of KC-HCFs, the expression of IL-6 was further amplified by the treatment with RB-PDT (p = 0.001). However, the activation of the MAPK and NF-κB pathways did not increase following RB-PDT. Additionally, RB-PDT reduced the transcription of collagen I and collagen V (p≤0.03), while the transcription of LOX and TGF-β1 secretion remained unchanged in KC-HCFs exposed to LPS.<h4>Conclusion</h4>In LPS-induced inflammation of KC-HCFs treated with RB-PDT, despite the increased expression of pro-inflammatory cytokines, the activation of the TLR4 signaling pathways is lacking. RB-PDT may have no adverse effects on corneal scar formation of keratoconus corneas in the short term.https://doi.org/10.1371/journal.pone.0318132 |
| spellingShingle | Ning Chai Tanja Stachon Sabrina Häcker Tim Berger Zhen Li Maryam Amini Shweta Suiwal Berthold Seitz Achim Langenbucher Nóra Szentmáry The cellular response of lipopolysaccharide-induced inflammation in keratoconus human corneal fibroblasts to RB-PDT: Insights into cytokines, chemokines and related signaling pathways. PLoS ONE |
| title | The cellular response of lipopolysaccharide-induced inflammation in keratoconus human corneal fibroblasts to RB-PDT: Insights into cytokines, chemokines and related signaling pathways. |
| title_full | The cellular response of lipopolysaccharide-induced inflammation in keratoconus human corneal fibroblasts to RB-PDT: Insights into cytokines, chemokines and related signaling pathways. |
| title_fullStr | The cellular response of lipopolysaccharide-induced inflammation in keratoconus human corneal fibroblasts to RB-PDT: Insights into cytokines, chemokines and related signaling pathways. |
| title_full_unstemmed | The cellular response of lipopolysaccharide-induced inflammation in keratoconus human corneal fibroblasts to RB-PDT: Insights into cytokines, chemokines and related signaling pathways. |
| title_short | The cellular response of lipopolysaccharide-induced inflammation in keratoconus human corneal fibroblasts to RB-PDT: Insights into cytokines, chemokines and related signaling pathways. |
| title_sort | cellular response of lipopolysaccharide induced inflammation in keratoconus human corneal fibroblasts to rb pdt insights into cytokines chemokines and related signaling pathways |
| url | https://doi.org/10.1371/journal.pone.0318132 |
| work_keys_str_mv | AT ningchai thecellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT tanjastachon thecellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT sabrinahacker thecellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT timberger thecellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT zhenli thecellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT maryamamini thecellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT shwetasuiwal thecellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT bertholdseitz thecellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT achimlangenbucher thecellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT noraszentmary thecellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT ningchai cellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT tanjastachon cellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT sabrinahacker cellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT timberger cellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT zhenli cellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT maryamamini cellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT shwetasuiwal cellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT bertholdseitz cellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT achimlangenbucher cellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways AT noraszentmary cellularresponseoflipopolysaccharideinducedinflammationinkeratoconushumancornealfibroblaststorbpdtinsightsintocytokineschemokinesandrelatedsignalingpathways |