De novo Genome Assembly and Annotation of 12 Fungi Associated with Fruit Tree Decline Syndrome in ON, Canada

Abstract Apple and stone fruit trees are vital components of Ontario’s agricultural landscape. However, since 2016, these trees have been facing alarming mortality rates, exhibiting symptoms collectively referred to as Fruit Tree Decline (FTD) and Rapid Apple Decline (RAD). Despite its widespread oc...

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Main Authors: Muhammad Sulman, Evgeny Ilyukhin, Oscar Villanueva, Hai D. T. Nguyen, Shawkat Ali, Walid Ellouze
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05192-5
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author Muhammad Sulman
Evgeny Ilyukhin
Oscar Villanueva
Hai D. T. Nguyen
Shawkat Ali
Walid Ellouze
author_facet Muhammad Sulman
Evgeny Ilyukhin
Oscar Villanueva
Hai D. T. Nguyen
Shawkat Ali
Walid Ellouze
author_sort Muhammad Sulman
collection DOAJ
description Abstract Apple and stone fruit trees are vital components of Ontario’s agricultural landscape. However, since 2016, these trees have been facing alarming mortality rates, exhibiting symptoms collectively referred to as Fruit Tree Decline (FTD) and Rapid Apple Decline (RAD). Despite its widespread occurrence, the exact cause of FTD and RAD remains elusive, with various pathogenic fungi and viruses implicated, along with abiotic stressors such as drought, winter injury and nutrient deficiency. In this study, we sequenced, assembled and annotated the genomes of 12 fungi associated with FTD and RAD syndromes in Ontario, Canada. We present the first and only publicly available assemblies for three ascomycete species including Diplodia intermedia, Diatrype stigma, and Nothophoma quercina. Additionally, we present high-quality reference genome sequences for Diplodia seriata, Didymella pomorum and Neofusicoccum ribis. These genomic resources are valuable for understanding the molecular mechanisms behind FTD and RAD, and for developing strategies for disease prevention and management in fruit trees.
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spelling doaj-art-8e1b7f8fb7644df396f9e23b410746072025-08-20T03:03:23ZengNature PortfolioScientific Data2052-44632025-07-0112111210.1038/s41597-025-05192-5De novo Genome Assembly and Annotation of 12 Fungi Associated with Fruit Tree Decline Syndrome in ON, CanadaMuhammad Sulman0Evgeny Ilyukhin1Oscar Villanueva2Hai D. T. Nguyen3Shawkat Ali4Walid Ellouze5Agriculture and Agri-Food CanadaAgriculture and Agri-Food CanadaAgriculture and Agri-Food CanadaAgriculture and Agri-Food CanadaAgriculture and Agri-Food CanadaAgriculture and Agri-Food CanadaAbstract Apple and stone fruit trees are vital components of Ontario’s agricultural landscape. However, since 2016, these trees have been facing alarming mortality rates, exhibiting symptoms collectively referred to as Fruit Tree Decline (FTD) and Rapid Apple Decline (RAD). Despite its widespread occurrence, the exact cause of FTD and RAD remains elusive, with various pathogenic fungi and viruses implicated, along with abiotic stressors such as drought, winter injury and nutrient deficiency. In this study, we sequenced, assembled and annotated the genomes of 12 fungi associated with FTD and RAD syndromes in Ontario, Canada. We present the first and only publicly available assemblies for three ascomycete species including Diplodia intermedia, Diatrype stigma, and Nothophoma quercina. Additionally, we present high-quality reference genome sequences for Diplodia seriata, Didymella pomorum and Neofusicoccum ribis. These genomic resources are valuable for understanding the molecular mechanisms behind FTD and RAD, and for developing strategies for disease prevention and management in fruit trees.https://doi.org/10.1038/s41597-025-05192-5
spellingShingle Muhammad Sulman
Evgeny Ilyukhin
Oscar Villanueva
Hai D. T. Nguyen
Shawkat Ali
Walid Ellouze
De novo Genome Assembly and Annotation of 12 Fungi Associated with Fruit Tree Decline Syndrome in ON, Canada
Scientific Data
title De novo Genome Assembly and Annotation of 12 Fungi Associated with Fruit Tree Decline Syndrome in ON, Canada
title_full De novo Genome Assembly and Annotation of 12 Fungi Associated with Fruit Tree Decline Syndrome in ON, Canada
title_fullStr De novo Genome Assembly and Annotation of 12 Fungi Associated with Fruit Tree Decline Syndrome in ON, Canada
title_full_unstemmed De novo Genome Assembly and Annotation of 12 Fungi Associated with Fruit Tree Decline Syndrome in ON, Canada
title_short De novo Genome Assembly and Annotation of 12 Fungi Associated with Fruit Tree Decline Syndrome in ON, Canada
title_sort de novo genome assembly and annotation of 12 fungi associated with fruit tree decline syndrome in on canada
url https://doi.org/10.1038/s41597-025-05192-5
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