Versatile High-Throughput Platform for Focused Ultrasound In Vitro Application
For the more efficient application of ultrasound in future therapies, fundamental research is needed on the mode of action of ultrasound on biological systems using therapeutic frequencies. To address this need, a new versatile high-throughput platform for focused ultrasound in vitro application was...
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MDPI AG
2025-01-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/15/2/847 |
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| author | Steffen H. Tretbar Marc Fournelle Christian Degel Franz-Josef Becker Peter Weber Sarah Therre-Mohr Wolfgang Bost Lisa Landgraf Andreas Melzer |
| author_facet | Steffen H. Tretbar Marc Fournelle Christian Degel Franz-Josef Becker Peter Weber Sarah Therre-Mohr Wolfgang Bost Lisa Landgraf Andreas Melzer |
| author_sort | Steffen H. Tretbar |
| collection | DOAJ |
| description | For the more efficient application of ultrasound in future therapies, fundamental research is needed on the mode of action of ultrasound on biological systems using therapeutic frequencies. To address this need, a new versatile high-throughput platform for focused ultrasound in vitro application was designed, developed, and characterized. The applicator was aligned with the dimensions of a 96-well plate and frequencies commonly used in the therapeutic ultrasound range (0.5–2.0 MHz). Two different platform configurations were developed: (a) a low-intensity version with 96 individual transducers allowing dry coupling of the well plate; and (b) a high-intensity version with water cooling, supporting parallel sonication of 32 out of 96 wells. The platforms were characterized by performing an analysis of the homogeneity of the sound pressure and intensity, the impact of filled volume per well, the cross-coupling effect between the wells, and the influence of the well plate. The low-intensity design delivers pressure levels up to 605 kPa inside the well with maximum I<sub>SPPA</sub> values between 0.78 and 12.38 W/cm<sup>2</sup>. In contrast, the high-intensity system achieves pressures up to 1460 kPa and a maximum I<sub>SPPA</sub> of 72 W/cm<sup>2</sup> inside the wells. The successfully developed high-throughput platform supports parallelized sonication in standard, well-plate formats and is suitable for focused ultrasound applications in vitro. |
| format | Article |
| id | doaj-art-8e12f227f01c40a9a61c380981652593 |
| institution | Kabale University |
| issn | 2076-3417 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj-art-8e12f227f01c40a9a61c3809816525932025-01-24T13:21:02ZengMDPI AGApplied Sciences2076-34172025-01-0115284710.3390/app15020847Versatile High-Throughput Platform for Focused Ultrasound In Vitro ApplicationSteffen H. Tretbar0Marc Fournelle1Christian Degel2Franz-Josef Becker3Peter Weber4Sarah Therre-Mohr5Wolfgang Bost6Lisa Landgraf7Andreas Melzer8Department Ultrasound, Fraunhofer Institute for Biomedical Engineering IBMT, 66280 Sulzbach, GermanyDepartment Ultrasound, Fraunhofer Institute for Biomedical Engineering IBMT, 66280 Sulzbach, GermanyDepartment Ultrasound, Fraunhofer Institute for Biomedical Engineering IBMT, 66280 Sulzbach, GermanyDepartment Ultrasound, Fraunhofer Institute for Biomedical Engineering IBMT, 66280 Sulzbach, GermanyDepartment Ultrasound, Fraunhofer Institute for Biomedical Engineering IBMT, 66280 Sulzbach, GermanyDepartment Ultrasound, Fraunhofer Institute for Biomedical Engineering IBMT, 66280 Sulzbach, GermanyDepartment Ultrasound, Fraunhofer Institute for Biomedical Engineering IBMT, 66280 Sulzbach, GermanyInnovation Center Computer Assisted Surgery, Faculty of Medicine, University of Leipzig, 04103 Leipzig, GermanyInnovation Center Computer Assisted Surgery, Faculty of Medicine, University of Leipzig, 04103 Leipzig, GermanyFor the more efficient application of ultrasound in future therapies, fundamental research is needed on the mode of action of ultrasound on biological systems using therapeutic frequencies. To address this need, a new versatile high-throughput platform for focused ultrasound in vitro application was designed, developed, and characterized. The applicator was aligned with the dimensions of a 96-well plate and frequencies commonly used in the therapeutic ultrasound range (0.5–2.0 MHz). Two different platform configurations were developed: (a) a low-intensity version with 96 individual transducers allowing dry coupling of the well plate; and (b) a high-intensity version with water cooling, supporting parallel sonication of 32 out of 96 wells. The platforms were characterized by performing an analysis of the homogeneity of the sound pressure and intensity, the impact of filled volume per well, the cross-coupling effect between the wells, and the influence of the well plate. The low-intensity design delivers pressure levels up to 605 kPa inside the well with maximum I<sub>SPPA</sub> values between 0.78 and 12.38 W/cm<sup>2</sup>. In contrast, the high-intensity system achieves pressures up to 1460 kPa and a maximum I<sub>SPPA</sub> of 72 W/cm<sup>2</sup> inside the wells. The successfully developed high-throughput platform supports parallelized sonication in standard, well-plate formats and is suitable for focused ultrasound applications in vitro.https://www.mdpi.com/2076-3417/15/2/847high-intensity focused ultrasoundin vitro setuplow-intensity focused ultrasoundtherapeutic ultrasoundversatile high-throughput platform |
| spellingShingle | Steffen H. Tretbar Marc Fournelle Christian Degel Franz-Josef Becker Peter Weber Sarah Therre-Mohr Wolfgang Bost Lisa Landgraf Andreas Melzer Versatile High-Throughput Platform for Focused Ultrasound In Vitro Application Applied Sciences high-intensity focused ultrasound in vitro setup low-intensity focused ultrasound therapeutic ultrasound versatile high-throughput platform |
| title | Versatile High-Throughput Platform for Focused Ultrasound In Vitro Application |
| title_full | Versatile High-Throughput Platform for Focused Ultrasound In Vitro Application |
| title_fullStr | Versatile High-Throughput Platform for Focused Ultrasound In Vitro Application |
| title_full_unstemmed | Versatile High-Throughput Platform for Focused Ultrasound In Vitro Application |
| title_short | Versatile High-Throughput Platform for Focused Ultrasound In Vitro Application |
| title_sort | versatile high throughput platform for focused ultrasound in vitro application |
| topic | high-intensity focused ultrasound in vitro setup low-intensity focused ultrasound therapeutic ultrasound versatile high-throughput platform |
| url | https://www.mdpi.com/2076-3417/15/2/847 |
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