NRF2 ACTIVATOR DIMETHYL FUMARATE DIMINISHED STEATOSIS, INFLAMMATION AND LIPID PEROXIDATION IN THE LIVER OF BINGE ETHANOL-TREATED RATS
Objective: This study was conducted to explore the impact of dimethyl fumarate (DMF), which has antioxidant and anti-inflammatory effects, on binge ethanol (EtOH)-induced hepatic steatosis, inflammation, and lipid peroxidation in rats.Material and Method: To examine the potential protective effect o...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Istanbul University Press
2024-01-01
|
| Series: | İstanbul Tıp Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/C69D8D8E34F84D2983EC84D6DF81E0A2 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849313352752300032 |
|---|---|
| author | İlknur Bingül Canan Küçükgergin Asiye Işın Doğan Ekici Semra Doğru Abbasoğlu Mehmet Müjdat Uysal |
| author_facet | İlknur Bingül Canan Küçükgergin Asiye Işın Doğan Ekici Semra Doğru Abbasoğlu Mehmet Müjdat Uysal |
| author_sort | İlknur Bingül |
| collection | DOAJ |
| description | Objective: This study was conducted to explore the impact of dimethyl fumarate (DMF), which has antioxidant and anti-inflammatory effects, on binge ethanol (EtOH)-induced hepatic steatosis, inflammation, and lipid peroxidation in rats.Material and Method: To examine the potential protective effect of DMF against EtOH-induced hepatic damage, rats were divided into four groups control, DMF, EtOH, and DMF+EtOH. Rats were administered EtOH (4.5 g/kg orally, 3 doses with 12-h intervals). DMF (30 mg/kg; gavage) was applied to rats 1 hour before each application of EtOH in the DMF+EtOH group. Serum markers of liver damage, triglyceride (TG), tumor necrosis factor-alpha (TNF-α), lipid and protein oxidation products, myeloperoxidase (MPO), and antioxidant enzymes together with histopathological examinations were performed in liver tissue. Protein expressions associated with antioxidant mechanism (nuclear factor erythroid 2-related factor; Nrf2 and heme oxygenase- 1; HO-1), lipid metabolism (sterol regulatory element-binding protein-1c; SREBP-1c and peroxisome proliferator-activated receptor-alpha; PPAR-α), oxidative stress (cytochrome P4502E1; CYP2E1), and inflammation (nuclear factor-kappa B; NF-κB) were also investigated in the rats’ livers. Result: DMF reduced elevated levels of serum markers of liver damage and hepatic TG, TNF-α and reactive oxygen species levels, lipid and protein oxidation products, and MPO activity together with the alleviation of histopathological lesions in EtOH-treated rats. Increased Nrf2 and HO-1 and decreased SREBP-1c and CYP2E1 expressions were also detected in the DMF+EtOH group compared with the EtOH group.Conclusion: Our results demonstrate that DMF may provide a protective effect against EtOH-induced hepatic lesions. These outcomes may be linked to the anti-oxidative, anti-inflammatory, and anti-lipogenic potential of DMF-induced Nrf2 activation. |
| format | Article |
| id | doaj-art-8e08b9595d4a4010a4f3f6fef629517f |
| institution | Kabale University |
| issn | 1305-6441 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | İstanbul Tıp Fakültesi Dergisi |
| spelling | doaj-art-8e08b9595d4a4010a4f3f6fef629517f2025-08-20T03:52:47ZengIstanbul University Pressİstanbul Tıp Fakültesi Dergisi1305-64412024-01-01871112010.26650/IUITFD.1344655123456NRF2 ACTIVATOR DIMETHYL FUMARATE DIMINISHED STEATOSIS, INFLAMMATION AND LIPID PEROXIDATION IN THE LIVER OF BINGE ETHANOL-TREATED RATSİlknur Bingül0https://orcid.org/0000-0002-6432-3541Canan Küçükgergin1https://orcid.org/0000-0002-1797-5889Asiye Işın Doğan Ekici2https://orcid.org/0000-0003-4062-9519Semra Doğru Abbasoğlu3https://orcid.org/0000-0003-3467-9763Mehmet Müjdat Uysal4https://orcid.org/0000-0002-8802-8766İstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, TürkiyeAcıbadem Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, TürkiyeObjective: This study was conducted to explore the impact of dimethyl fumarate (DMF), which has antioxidant and anti-inflammatory effects, on binge ethanol (EtOH)-induced hepatic steatosis, inflammation, and lipid peroxidation in rats.Material and Method: To examine the potential protective effect of DMF against EtOH-induced hepatic damage, rats were divided into four groups control, DMF, EtOH, and DMF+EtOH. Rats were administered EtOH (4.5 g/kg orally, 3 doses with 12-h intervals). DMF (30 mg/kg; gavage) was applied to rats 1 hour before each application of EtOH in the DMF+EtOH group. Serum markers of liver damage, triglyceride (TG), tumor necrosis factor-alpha (TNF-α), lipid and protein oxidation products, myeloperoxidase (MPO), and antioxidant enzymes together with histopathological examinations were performed in liver tissue. Protein expressions associated with antioxidant mechanism (nuclear factor erythroid 2-related factor; Nrf2 and heme oxygenase- 1; HO-1), lipid metabolism (sterol regulatory element-binding protein-1c; SREBP-1c and peroxisome proliferator-activated receptor-alpha; PPAR-α), oxidative stress (cytochrome P4502E1; CYP2E1), and inflammation (nuclear factor-kappa B; NF-κB) were also investigated in the rats’ livers. Result: DMF reduced elevated levels of serum markers of liver damage and hepatic TG, TNF-α and reactive oxygen species levels, lipid and protein oxidation products, and MPO activity together with the alleviation of histopathological lesions in EtOH-treated rats. Increased Nrf2 and HO-1 and decreased SREBP-1c and CYP2E1 expressions were also detected in the DMF+EtOH group compared with the EtOH group.Conclusion: Our results demonstrate that DMF may provide a protective effect against EtOH-induced hepatic lesions. These outcomes may be linked to the anti-oxidative, anti-inflammatory, and anti-lipogenic potential of DMF-induced Nrf2 activation.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/C69D8D8E34F84D2983EC84D6DF81E0A2dimethyl fumaratenuclear factor erythroid 2-related factorethanolliver damageoxidative stress |
| spellingShingle | İlknur Bingül Canan Küçükgergin Asiye Işın Doğan Ekici Semra Doğru Abbasoğlu Mehmet Müjdat Uysal NRF2 ACTIVATOR DIMETHYL FUMARATE DIMINISHED STEATOSIS, INFLAMMATION AND LIPID PEROXIDATION IN THE LIVER OF BINGE ETHANOL-TREATED RATS İstanbul Tıp Fakültesi Dergisi dimethyl fumarate nuclear factor erythroid 2-related factor ethanol liver damage oxidative stress |
| title | NRF2 ACTIVATOR DIMETHYL FUMARATE DIMINISHED STEATOSIS, INFLAMMATION AND LIPID PEROXIDATION IN THE LIVER OF BINGE ETHANOL-TREATED RATS |
| title_full | NRF2 ACTIVATOR DIMETHYL FUMARATE DIMINISHED STEATOSIS, INFLAMMATION AND LIPID PEROXIDATION IN THE LIVER OF BINGE ETHANOL-TREATED RATS |
| title_fullStr | NRF2 ACTIVATOR DIMETHYL FUMARATE DIMINISHED STEATOSIS, INFLAMMATION AND LIPID PEROXIDATION IN THE LIVER OF BINGE ETHANOL-TREATED RATS |
| title_full_unstemmed | NRF2 ACTIVATOR DIMETHYL FUMARATE DIMINISHED STEATOSIS, INFLAMMATION AND LIPID PEROXIDATION IN THE LIVER OF BINGE ETHANOL-TREATED RATS |
| title_short | NRF2 ACTIVATOR DIMETHYL FUMARATE DIMINISHED STEATOSIS, INFLAMMATION AND LIPID PEROXIDATION IN THE LIVER OF BINGE ETHANOL-TREATED RATS |
| title_sort | nrf2 activator dimethyl fumarate diminished steatosis inflammation and lipid peroxidation in the liver of binge ethanol treated rats |
| topic | dimethyl fumarate nuclear factor erythroid 2-related factor ethanol liver damage oxidative stress |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/C69D8D8E34F84D2983EC84D6DF81E0A2 |
| work_keys_str_mv | AT ilknurbingul nrf2activatordimethylfumaratediminishedsteatosisinflammationandlipidperoxidationintheliverofbingeethanoltreatedrats AT canankucukgergin nrf2activatordimethylfumaratediminishedsteatosisinflammationandlipidperoxidationintheliverofbingeethanoltreatedrats AT asiyeisındoganekici nrf2activatordimethylfumaratediminishedsteatosisinflammationandlipidperoxidationintheliverofbingeethanoltreatedrats AT semradogruabbasoglu nrf2activatordimethylfumaratediminishedsteatosisinflammationandlipidperoxidationintheliverofbingeethanoltreatedrats AT mehmetmujdatuysal nrf2activatordimethylfumaratediminishedsteatosisinflammationandlipidperoxidationintheliverofbingeethanoltreatedrats |