Biotransformation of Hydrocortisone by Neurospora crassa
The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral...
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| Format: | Article |
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| Language: | English |
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University of Tehran
2006-12-01
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| Series: | Journal of Sciences, Islamic Republic of Iran |
| Online Access: | https://jsciences.ut.ac.ir/article_31771_37aa7a76522050e63c19968c72f51634.pdf |
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| description | The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-hydroxyandrost-4-en-3,17-dione (III) in the yields of 25% and 18.8%, respectively. Time course study showed the accumulation of both products from the second day of the fermentation and reached to the maximum in the seventh day. Optimum concentration of the substrate, which gave maximum bioconversion efficiency, was 1 mg ml?1 in one batch. Biotransformation was completely inhibited in a concentration above 2.5 mg ml?1. |
| format | Article |
| id | doaj-art-8e0761616636400787254f29b44cc339 |
| institution | OA Journals |
| issn | 1016-1104 2345-6914 |
| language | English |
| publishDate | 2006-12-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Journal of Sciences, Islamic Republic of Iran |
| spelling | doaj-art-8e0761616636400787254f29b44cc3392025-08-20T01:53:09ZengUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-11042345-69142006-12-0117431771Biotransformation of Hydrocortisone by Neurospora crassaThe ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-hydroxyandrost-4-en-3,17-dione (III) in the yields of 25% and 18.8%, respectively. Time course study showed the accumulation of both products from the second day of the fermentation and reached to the maximum in the seventh day. Optimum concentration of the substrate, which gave maximum bioconversion efficiency, was 1 mg ml?1 in one batch. Biotransformation was completely inhibited in a concentration above 2.5 mg ml?1.https://jsciences.ut.ac.ir/article_31771_37aa7a76522050e63c19968c72f51634.pdf |
| spellingShingle | Biotransformation of Hydrocortisone
by Neurospora crassa Journal of Sciences, Islamic Republic of Iran |
| title | Biotransformation of Hydrocortisone
by Neurospora crassa |
| title_full | Biotransformation of Hydrocortisone
by Neurospora crassa |
| title_fullStr | Biotransformation of Hydrocortisone
by Neurospora crassa |
| title_full_unstemmed | Biotransformation of Hydrocortisone
by Neurospora crassa |
| title_short | Biotransformation of Hydrocortisone
by Neurospora crassa |
| title_sort | biotransformation of hydrocortisone by neurospora crassa |
| url | https://jsciences.ut.ac.ir/article_31771_37aa7a76522050e63c19968c72f51634.pdf |