In vitro and molecular docking evaluation of black sesame seeds' anti-prostate cancer and antioxidant activity processed by nine steaming nine drying
Abstract Black sesame seeds, known for their rich flavor and medicinal properties, hold significant potential as natural therapeutics against prostate cancer, a major health challenge for men today. This study explores the traditional processing technique of nine cycles of steaming and drying, which...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-01-01
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| Series: | Applied Biological Chemistry |
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| Online Access: | https://doi.org/10.1186/s13765-024-00965-9 |
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| author | Glennielyn D. Magdamit Cheng-Yang Hsieh Mon-Juan Lee Kathlia A. De Castro-Cruz Samantha Franchette B. Austria Beatrice D. Sipat Steven Kuan-Hua Huang Po-Wei Tsai |
| author_facet | Glennielyn D. Magdamit Cheng-Yang Hsieh Mon-Juan Lee Kathlia A. De Castro-Cruz Samantha Franchette B. Austria Beatrice D. Sipat Steven Kuan-Hua Huang Po-Wei Tsai |
| author_sort | Glennielyn D. Magdamit |
| collection | DOAJ |
| description | Abstract Black sesame seeds, known for their rich flavor and medicinal properties, hold significant potential as natural therapeutics against prostate cancer, a major health challenge for men today. This study explores the traditional processing technique of nine cycles of steaming and drying, which enhances the bioactive potential of these seeds. The impact of this processing on the antioxidant and anti-prostate cancer properties of black sesame seeds was systematically investigated, focusing on the key lignans, sesamin and sesamolin. HPLC was utilized to analyze the content ratios of sesamin and sesamolin, while DPPH and FRAP assays evaluated antioxidant capabilities, and MTT assays assessed anti-cancer properties against DU145 cells. Findings reveal that three cycles of steaming and drying significantly enhance antioxidant and anti-cancer activities against DU145, achieving peak concentrations of sesamin and sesamolin of 21.583% and 14.991%, respectively, with an optimal ratio of 1.4397:1. The superior antioxidant and anti-prostate cancer activity of this sample is attributed to optimal processing conditions that maximize the stability and extraction of bioactive compounds, particularly non-lignan antioxidants, while minimizing degradation; this is likely enhanced by the interplay between various phytochemicals and the effects of thermal processing on cellular structure. Processed seeds consistently outperformed raw seeds—except for those subjected to a single cycle. Additionally, molecular docking analyses revealed compelling interactions between sesamin and sesamolin and key proteins implicated in prostate cancer (FYN, ITGB3, PDGFRA, PDGFRB, and PIK3R1), demonstrating higher LibDock scores than the standard anti-cancer drug 5-fluorouracil. This research highlights the exceptional antioxidant and anti-cancer potential of black sesame seeds, particularly through the three-steaming and three-drying method, emphasizing the importance of the sesamin to sesamolin ratio in developing future anti-cancer therapeutics. |
| format | Article |
| id | doaj-art-8e04ce30b31a41b2aef4c9e46a9c774a |
| institution | Kabale University |
| issn | 2468-0842 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Applied Biological Chemistry |
| spelling | doaj-art-8e04ce30b31a41b2aef4c9e46a9c774a2025-01-19T12:36:38ZengSpringerOpenApplied Biological Chemistry2468-08422025-01-0168111710.1186/s13765-024-00965-9In vitro and molecular docking evaluation of black sesame seeds' anti-prostate cancer and antioxidant activity processed by nine steaming nine dryingGlennielyn D. Magdamit0Cheng-Yang Hsieh1Mon-Juan Lee2Kathlia A. De Castro-Cruz3Samantha Franchette B. Austria4Beatrice D. Sipat5Steven Kuan-Hua Huang6Po-Wei Tsai7School of Chemical, Biological, and Materials Engineering and Sciences, Mapúa UniversityDepartment of Food Science, National Taiwan Ocean UniversityDepartment of Medical Science Industries, College of Health Sciences, Chang Jung Christian UniversitySchool of Chemical, Biological, and Materials Engineering and Sciences, Mapúa UniversitySchool of Chemical, Biological, and Materials Engineering and Sciences, Mapúa UniversitySchool of Chemical, Biological, and Materials Engineering and Sciences, Mapúa UniversityDivision of Urology, Department of Surgery, Chi Mei Medical CenterDepartment of Food Science, National Taiwan Ocean UniversityAbstract Black sesame seeds, known for their rich flavor and medicinal properties, hold significant potential as natural therapeutics against prostate cancer, a major health challenge for men today. This study explores the traditional processing technique of nine cycles of steaming and drying, which enhances the bioactive potential of these seeds. The impact of this processing on the antioxidant and anti-prostate cancer properties of black sesame seeds was systematically investigated, focusing on the key lignans, sesamin and sesamolin. HPLC was utilized to analyze the content ratios of sesamin and sesamolin, while DPPH and FRAP assays evaluated antioxidant capabilities, and MTT assays assessed anti-cancer properties against DU145 cells. Findings reveal that three cycles of steaming and drying significantly enhance antioxidant and anti-cancer activities against DU145, achieving peak concentrations of sesamin and sesamolin of 21.583% and 14.991%, respectively, with an optimal ratio of 1.4397:1. The superior antioxidant and anti-prostate cancer activity of this sample is attributed to optimal processing conditions that maximize the stability and extraction of bioactive compounds, particularly non-lignan antioxidants, while minimizing degradation; this is likely enhanced by the interplay between various phytochemicals and the effects of thermal processing on cellular structure. Processed seeds consistently outperformed raw seeds—except for those subjected to a single cycle. Additionally, molecular docking analyses revealed compelling interactions between sesamin and sesamolin and key proteins implicated in prostate cancer (FYN, ITGB3, PDGFRA, PDGFRB, and PIK3R1), demonstrating higher LibDock scores than the standard anti-cancer drug 5-fluorouracil. This research highlights the exceptional antioxidant and anti-cancer potential of black sesame seeds, particularly through the three-steaming and three-drying method, emphasizing the importance of the sesamin to sesamolin ratio in developing future anti-cancer therapeutics.https://doi.org/10.1186/s13765-024-00965-9AntioxidantBlack sesame seedsMolecular dockingNine-steaming-nine-dryingProstate cancer |
| spellingShingle | Glennielyn D. Magdamit Cheng-Yang Hsieh Mon-Juan Lee Kathlia A. De Castro-Cruz Samantha Franchette B. Austria Beatrice D. Sipat Steven Kuan-Hua Huang Po-Wei Tsai In vitro and molecular docking evaluation of black sesame seeds' anti-prostate cancer and antioxidant activity processed by nine steaming nine drying Applied Biological Chemistry Antioxidant Black sesame seeds Molecular docking Nine-steaming-nine-drying Prostate cancer |
| title | In vitro and molecular docking evaluation of black sesame seeds' anti-prostate cancer and antioxidant activity processed by nine steaming nine drying |
| title_full | In vitro and molecular docking evaluation of black sesame seeds' anti-prostate cancer and antioxidant activity processed by nine steaming nine drying |
| title_fullStr | In vitro and molecular docking evaluation of black sesame seeds' anti-prostate cancer and antioxidant activity processed by nine steaming nine drying |
| title_full_unstemmed | In vitro and molecular docking evaluation of black sesame seeds' anti-prostate cancer and antioxidant activity processed by nine steaming nine drying |
| title_short | In vitro and molecular docking evaluation of black sesame seeds' anti-prostate cancer and antioxidant activity processed by nine steaming nine drying |
| title_sort | in vitro and molecular docking evaluation of black sesame seeds anti prostate cancer and antioxidant activity processed by nine steaming nine drying |
| topic | Antioxidant Black sesame seeds Molecular docking Nine-steaming-nine-drying Prostate cancer |
| url | https://doi.org/10.1186/s13765-024-00965-9 |
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