Screening of Postbiotics against Salmonella and Whole Genome Analysis of the Original Strain
Objective: To screen postbiotics with exceptional anti-Salmonella activity and develop postbiotic raw materials with industrialization value. Methods: The anti-Salmonella potential of 30 Lactobacillus postbiotic strains was evaluated, focusing on their ability to inhibit the growth of Salmonella ent...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2025-03-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024040240 |
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| author | Yan HUANG Yue SU Shiyang LI Xuehe QI Ai JIA Yujun JIANG Xinyan YANG Chaoxin MAN |
| author_facet | Yan HUANG Yue SU Shiyang LI Xuehe QI Ai JIA Yujun JIANG Xinyan YANG Chaoxin MAN |
| author_sort | Yan HUANG |
| collection | DOAJ |
| description | Objective: To screen postbiotics with exceptional anti-Salmonella activity and develop postbiotic raw materials with industrialization value. Methods: The anti-Salmonella potential of 30 Lactobacillus postbiotic strains was evaluated, focusing on their ability to inhibit the growth of Salmonella enterica serovar Typhimurium (ST). The top five most effective strains were identified, and further assays were conducted to assess their capacity to inhibit ST adhesion and invasion in HT-29 cells. The most significant effective postbiotic was selected for whole genome sequencing and safety evaluation of its originating strain. Results: Lactobacillus plantarum (L. plantarum) JM015 postbiotic exhibited a notable inhibition zone diameter of 15.12±0.53 mm. It effectively reduced the adhesion and invasion of Salmonella to HT-29 cells by more than 90%. Whole genome sequencing of L. plantarum JM015 revealed four bacteriostasis-related gene clusters related to repression: cyclic-lactone-autoinducer, terpene, ribosomally synthesized and post-translationally modified peptide (RiPP)-like, and type III polyketide synthases (PKS). Additionally, it harbors a gene cluster associated with plantaricin production and several genes linked to adhesion, such as fibronectin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and triosephosphate isomerase. The safety of the original strain was confirmed through tests for indole production, hemolysis, and nitrate reductase activity. Conclusion: The L. plantarum JM015 postbiotic demonstrates potent anti-Salmonella activity and can as a novel postbiotic ingredient for product development. |
| format | Article |
| id | doaj-art-8e02eb35933c4ca1a2d4c922d84f264c |
| institution | DOAJ |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-03-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-8e02eb35933c4ca1a2d4c922d84f264c2025-08-20T02:47:40ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-03-0146619520510.13386/j.issn1002-0306.20240402402024040240-6Screening of Postbiotics against Salmonella and Whole Genome Analysis of the Original StrainYan HUANG0Yue SU1Shiyang LI2Xuehe QI3Ai JIA4Yujun JIANG5Xinyan YANG6Chaoxin MAN7College of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaHeilongjiang Institute of Quality Supervision and Testing, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaObjective: To screen postbiotics with exceptional anti-Salmonella activity and develop postbiotic raw materials with industrialization value. Methods: The anti-Salmonella potential of 30 Lactobacillus postbiotic strains was evaluated, focusing on their ability to inhibit the growth of Salmonella enterica serovar Typhimurium (ST). The top five most effective strains were identified, and further assays were conducted to assess their capacity to inhibit ST adhesion and invasion in HT-29 cells. The most significant effective postbiotic was selected for whole genome sequencing and safety evaluation of its originating strain. Results: Lactobacillus plantarum (L. plantarum) JM015 postbiotic exhibited a notable inhibition zone diameter of 15.12±0.53 mm. It effectively reduced the adhesion and invasion of Salmonella to HT-29 cells by more than 90%. Whole genome sequencing of L. plantarum JM015 revealed four bacteriostasis-related gene clusters related to repression: cyclic-lactone-autoinducer, terpene, ribosomally synthesized and post-translationally modified peptide (RiPP)-like, and type III polyketide synthases (PKS). Additionally, it harbors a gene cluster associated with plantaricin production and several genes linked to adhesion, such as fibronectin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and triosephosphate isomerase. The safety of the original strain was confirmed through tests for indole production, hemolysis, and nitrate reductase activity. Conclusion: The L. plantarum JM015 postbiotic demonstrates potent anti-Salmonella activity and can as a novel postbiotic ingredient for product development.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024040240lactobacilluspostbioticsalmonella enterica serovar typhimuriumantibacterialwhole-genomesafety evaluation |
| spellingShingle | Yan HUANG Yue SU Shiyang LI Xuehe QI Ai JIA Yujun JIANG Xinyan YANG Chaoxin MAN Screening of Postbiotics against Salmonella and Whole Genome Analysis of the Original Strain Shipin gongye ke-ji lactobacillus postbiotic salmonella enterica serovar typhimurium antibacterial whole-genome safety evaluation |
| title | Screening of Postbiotics against Salmonella and Whole Genome Analysis of the Original Strain |
| title_full | Screening of Postbiotics against Salmonella and Whole Genome Analysis of the Original Strain |
| title_fullStr | Screening of Postbiotics against Salmonella and Whole Genome Analysis of the Original Strain |
| title_full_unstemmed | Screening of Postbiotics against Salmonella and Whole Genome Analysis of the Original Strain |
| title_short | Screening of Postbiotics against Salmonella and Whole Genome Analysis of the Original Strain |
| title_sort | screening of postbiotics against salmonella and whole genome analysis of the original strain |
| topic | lactobacillus postbiotic salmonella enterica serovar typhimurium antibacterial whole-genome safety evaluation |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024040240 |
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