Genomic Analyses of Methicillin-Resistant <i>Staphylococcus pseudintermedius</i> from Companion Animals Reveal Changing Clonal Populations, Multidrug Resistance, and Virulence
Background/Objectives: <i>Staphylococcus pseudintermedius</i> is part of the normal microbiota in dogs. Since 2006, an increase in multidrug-resistant clones of methicillin-resistant <i>S. pseudintermedius</i> has been reported, as well as zoonotic transmission. Longitudinal...
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MDPI AG
2024-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/13/10/962 |
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| author | Mattias Myrenås Karl Pedersen Ulrika Windahl |
| author_facet | Mattias Myrenås Karl Pedersen Ulrika Windahl |
| author_sort | Mattias Myrenås |
| collection | DOAJ |
| description | Background/Objectives: <i>Staphylococcus pseudintermedius</i> is part of the normal microbiota in dogs. Since 2006, an increase in multidrug-resistant clones of methicillin-resistant <i>S. pseudintermedius</i> has been reported, as well as zoonotic transmission. Longitudinal investigations into clonal population structures, antibiotic resistance patterns, and the presence of resistance and virulence genes are important tools for gaining knowledge of the mechanisms behind the emergence of such clones. Methods: We investigated 87% of all non-repetitive MRSP isolates from dogs and cats in Sweden over a ten-year period (n = 356). All isolates were subjected to staphylococcal chromosomal cassette <i>mec</i> identification, whole-genome sequencing, multi-locus sequence typing, and analyses of genomic relatedness, as well as investigation of phenotypical resistance patterns and the presence of antibiotic resistance genes and virulence genes. Results: A considerable increase over time in the number of clonal lineages present was observed, indicating genomic diversification, and four clones became dominant: ST71, ST258, ST265, and ST551. In total, 96% of the isolates were multidrug-resistant. Statistically significant differences in resistance to several antibiotic classes between the four dominant clones were present. All isolates carried several virulence genes encoding factors associated with attachment, colonization, toxin synthesis, quorum sensing, antibiotic resistance, and immune evasion. |
| format | Article |
| id | doaj-art-8e00fe9d63844bc8a0c087e0b419d7b1 |
| institution | OA Journals |
| issn | 2079-6382 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-8e00fe9d63844bc8a0c087e0b419d7b12025-08-20T02:11:04ZengMDPI AGAntibiotics2079-63822024-10-01131096210.3390/antibiotics13100962Genomic Analyses of Methicillin-Resistant <i>Staphylococcus pseudintermedius</i> from Companion Animals Reveal Changing Clonal Populations, Multidrug Resistance, and VirulenceMattias Myrenås0Karl Pedersen1Ulrika Windahl2Swedish Veterinary Agency, Ulls väg 2b, SE-75189 Uppsala, SwedenDepartment of Animal and Veterinary Science, Aarhus University, Blichers Allé 20, 8830 Tjele, DenmarkSwedish Veterinary Agency, Ulls väg 2b, SE-75189 Uppsala, SwedenBackground/Objectives: <i>Staphylococcus pseudintermedius</i> is part of the normal microbiota in dogs. Since 2006, an increase in multidrug-resistant clones of methicillin-resistant <i>S. pseudintermedius</i> has been reported, as well as zoonotic transmission. Longitudinal investigations into clonal population structures, antibiotic resistance patterns, and the presence of resistance and virulence genes are important tools for gaining knowledge of the mechanisms behind the emergence of such clones. Methods: We investigated 87% of all non-repetitive MRSP isolates from dogs and cats in Sweden over a ten-year period (n = 356). All isolates were subjected to staphylococcal chromosomal cassette <i>mec</i> identification, whole-genome sequencing, multi-locus sequence typing, and analyses of genomic relatedness, as well as investigation of phenotypical resistance patterns and the presence of antibiotic resistance genes and virulence genes. Results: A considerable increase over time in the number of clonal lineages present was observed, indicating genomic diversification, and four clones became dominant: ST71, ST258, ST265, and ST551. In total, 96% of the isolates were multidrug-resistant. Statistically significant differences in resistance to several antibiotic classes between the four dominant clones were present. All isolates carried several virulence genes encoding factors associated with attachment, colonization, toxin synthesis, quorum sensing, antibiotic resistance, and immune evasion.https://www.mdpi.com/2079-6382/13/10/962MRSPclonal lineagescaninedogmultidrug-resistant staphylococci<i>SCCmec</i> |
| spellingShingle | Mattias Myrenås Karl Pedersen Ulrika Windahl Genomic Analyses of Methicillin-Resistant <i>Staphylococcus pseudintermedius</i> from Companion Animals Reveal Changing Clonal Populations, Multidrug Resistance, and Virulence Antibiotics MRSP clonal lineages canine dog multidrug-resistant staphylococci <i>SCCmec</i> |
| title | Genomic Analyses of Methicillin-Resistant <i>Staphylococcus pseudintermedius</i> from Companion Animals Reveal Changing Clonal Populations, Multidrug Resistance, and Virulence |
| title_full | Genomic Analyses of Methicillin-Resistant <i>Staphylococcus pseudintermedius</i> from Companion Animals Reveal Changing Clonal Populations, Multidrug Resistance, and Virulence |
| title_fullStr | Genomic Analyses of Methicillin-Resistant <i>Staphylococcus pseudintermedius</i> from Companion Animals Reveal Changing Clonal Populations, Multidrug Resistance, and Virulence |
| title_full_unstemmed | Genomic Analyses of Methicillin-Resistant <i>Staphylococcus pseudintermedius</i> from Companion Animals Reveal Changing Clonal Populations, Multidrug Resistance, and Virulence |
| title_short | Genomic Analyses of Methicillin-Resistant <i>Staphylococcus pseudintermedius</i> from Companion Animals Reveal Changing Clonal Populations, Multidrug Resistance, and Virulence |
| title_sort | genomic analyses of methicillin resistant i staphylococcus pseudintermedius i from companion animals reveal changing clonal populations multidrug resistance and virulence |
| topic | MRSP clonal lineages canine dog multidrug-resistant staphylococci <i>SCCmec</i> |
| url | https://www.mdpi.com/2079-6382/13/10/962 |
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