Ring-finger protein RNF126 promotes prostate cancer progression via regulation of MBNL1
Abstract Purpose Aberrant activation/overexpression of RNF126 is implicated as a driving event in tumor progression. However, although some functions of RNF126 in prostate cancer (PCa) cell lines has been reported, more biological functions and in-depth mechanisms should be further clarified in PCa....
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-04629-6 |
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| author | Xin Jiang Ji Li Jiali Zhang Yulei Zhao Guoqin He Xiaohui Yao |
| author_facet | Xin Jiang Ji Li Jiali Zhang Yulei Zhao Guoqin He Xiaohui Yao |
| author_sort | Xin Jiang |
| collection | DOAJ |
| description | Abstract Purpose Aberrant activation/overexpression of RNF126 is implicated as a driving event in tumor progression. However, although some functions of RNF126 in prostate cancer (PCa) cell lines has been reported, more biological functions and in-depth mechanisms should be further clarified in PCa. Methods Here, we provide evidence that RNF126 expression is elevated in human PCa tissues and cell lines, which is associated with tumor grades and prognosis. Cell proliferation was measured by the CCK8 and colony-formation assays. Cell migration was performed by Transwell and wound-healing assays. RNF126 target proteins were investigated via proteomic, co-immunoprecipitation and western blot methods. Additionally, we knock-downed MBNL1 expression to perform rescue experiments. In vivo, xenograft mice assay was used to verify the effect of RNF126 on the growth of PCa cell. Results Here, we showed that RNF126 was highly expressed in PCa and its higher expression was associated with worse patients’ prognosis. Expression modulation of RNF126 affects PCa cells proliferation, migration, EMT and docetaxel (DTX) resistance in vitro or in vivo. Additionally, RNF126 involves in the regulation of PI3K/AKT, MEK/ERK and EMT pathways. Mechanistically, immunoprecipitation (IP) and coimmunoprecipitation (co-IP) assays indicated that RNF126 could bind to MBNL1 directly. Our data also suggested that MBNL1 was a critical downstream event in RNF126-mediated tumorigenesis and chemo-resistance and played a crucial role in driving the PI3K/AKT, MEK/ERK and EMT pathways. Conclusion Taken together, our findings reveal a novel biological and molecular functions of RNF126 and may provide a new treatment option for PCa patients. |
| format | Article |
| id | doaj-art-8df142e7d08c4de29014a59f659ecf09 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-8df142e7d08c4de29014a59f659ecf092025-08-20T03:45:28ZengNature PortfolioScientific Reports2045-23222025-07-0115111310.1038/s41598-025-04629-6Ring-finger protein RNF126 promotes prostate cancer progression via regulation of MBNL1Xin Jiang0Ji Li1Jiali Zhang2Yulei Zhao3Guoqin He4Xiaohui Yao5Baoying People’s HospitalBaoying People’s HospitalKunming Municipal Hospital of Traditional Chinese Medicine, The Third Affiliated Hospital of Yunnan University of Chinese MedicineNanjing Jinling Hospital, Affiliated Hospital of Medical School, Nanjing UniversityBaoying People’s HospitalBaoying People’s HospitalAbstract Purpose Aberrant activation/overexpression of RNF126 is implicated as a driving event in tumor progression. However, although some functions of RNF126 in prostate cancer (PCa) cell lines has been reported, more biological functions and in-depth mechanisms should be further clarified in PCa. Methods Here, we provide evidence that RNF126 expression is elevated in human PCa tissues and cell lines, which is associated with tumor grades and prognosis. Cell proliferation was measured by the CCK8 and colony-formation assays. Cell migration was performed by Transwell and wound-healing assays. RNF126 target proteins were investigated via proteomic, co-immunoprecipitation and western blot methods. Additionally, we knock-downed MBNL1 expression to perform rescue experiments. In vivo, xenograft mice assay was used to verify the effect of RNF126 on the growth of PCa cell. Results Here, we showed that RNF126 was highly expressed in PCa and its higher expression was associated with worse patients’ prognosis. Expression modulation of RNF126 affects PCa cells proliferation, migration, EMT and docetaxel (DTX) resistance in vitro or in vivo. Additionally, RNF126 involves in the regulation of PI3K/AKT, MEK/ERK and EMT pathways. Mechanistically, immunoprecipitation (IP) and coimmunoprecipitation (co-IP) assays indicated that RNF126 could bind to MBNL1 directly. Our data also suggested that MBNL1 was a critical downstream event in RNF126-mediated tumorigenesis and chemo-resistance and played a crucial role in driving the PI3K/AKT, MEK/ERK and EMT pathways. Conclusion Taken together, our findings reveal a novel biological and molecular functions of RNF126 and may provide a new treatment option for PCa patients.https://doi.org/10.1038/s41598-025-04629-6Prostate cancerRNF126MBNL1Signaling pathwaysChemo-resistancePrognosis |
| spellingShingle | Xin Jiang Ji Li Jiali Zhang Yulei Zhao Guoqin He Xiaohui Yao Ring-finger protein RNF126 promotes prostate cancer progression via regulation of MBNL1 Scientific Reports Prostate cancer RNF126 MBNL1 Signaling pathways Chemo-resistance Prognosis |
| title | Ring-finger protein RNF126 promotes prostate cancer progression via regulation of MBNL1 |
| title_full | Ring-finger protein RNF126 promotes prostate cancer progression via regulation of MBNL1 |
| title_fullStr | Ring-finger protein RNF126 promotes prostate cancer progression via regulation of MBNL1 |
| title_full_unstemmed | Ring-finger protein RNF126 promotes prostate cancer progression via regulation of MBNL1 |
| title_short | Ring-finger protein RNF126 promotes prostate cancer progression via regulation of MBNL1 |
| title_sort | ring finger protein rnf126 promotes prostate cancer progression via regulation of mbnl1 |
| topic | Prostate cancer RNF126 MBNL1 Signaling pathways Chemo-resistance Prognosis |
| url | https://doi.org/10.1038/s41598-025-04629-6 |
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