Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test
Dimethyl sulfoxide (DMSO) is an amphipathic solvent for molecules in in vitro tests for detection of anthelmintic resistance of gastrointestinal nematodes (GIN). It has been shown to have a concentration-dependent detrimental effect on Caenorhabditis elegans, a free-living nematode. If GIN are likew...
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| Format: | Article |
| Language: | English |
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Sciendo
2025-03-01
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| Series: | Journal of Veterinary Research |
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| Online Access: | https://doi.org/10.2478/jvetres-2025-0016 |
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| author | Mickiewicz Marcin Nowek Zofia Czopowicz Michał Moroz-Fik Agata Potărniche Adrian-Valentin Biernacka Kinga Szaluś-Jordanow Olga Górski Paweł Antonopoulos Alistair Markowska-Daniel Iwona Várady Marián Kaba Jarosław |
| author_facet | Mickiewicz Marcin Nowek Zofia Czopowicz Michał Moroz-Fik Agata Potărniche Adrian-Valentin Biernacka Kinga Szaluś-Jordanow Olga Górski Paweł Antonopoulos Alistair Markowska-Daniel Iwona Várady Marián Kaba Jarosław |
| author_sort | Mickiewicz Marcin |
| collection | DOAJ |
| description | Dimethyl sulfoxide (DMSO) is an amphipathic solvent for molecules in in vitro tests for detection of anthelmintic resistance of gastrointestinal nematodes (GIN). It has been shown to have a concentration-dependent detrimental effect on Caenorhabditis elegans, a free-living nematode. If GIN are likewise affected, using DMSO in egg-hatch test and larval development test (LDT) may confound their results. Therefore, the DMSO concentration was determined at which it exerted an inhibitory effect on GIN larval development to the third stage. |
| format | Article |
| id | doaj-art-8def6b20f43241ad8ca6ddd81d04246a |
| institution | OA Journals |
| issn | 2450-8608 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Veterinary Research |
| spelling | doaj-art-8def6b20f43241ad8ca6ddd81d04246a2025-08-20T02:28:05ZengSciendoJournal of Veterinary Research2450-86082025-03-01691839010.2478/jvetres-2025-0016Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development testMickiewicz Marcin0Nowek Zofia1Czopowicz Michał2Moroz-Fik Agata3Potărniche Adrian-Valentin4Biernacka Kinga5Szaluś-Jordanow Olga6Górski Paweł7Antonopoulos Alistair8Markowska-Daniel Iwona9Várady Marián10Kaba Jarosław11Division of Veterinary Epidemiology and EconomicsDivision of Veterinary Epidemiology and EconomicsDivision of Veterinary Epidemiology and EconomicsDivision of Veterinary Epidemiology and EconomicsDepartment of Infectious Diseases and Preventive Medicine, Law and Ethics, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca400372, RomaniaDivision of Veterinary Epidemiology and EconomicsDepartment of Small Animal Diseases with Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences–SGGW, 02-776Warsaw, PolandDivision of Parasitology and Invasiology, Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences–SGGW, 02-786Warsaw, PolandKreavet, 9150Kruibeke, BelgiumDivision of Veterinary Epidemiology and EconomicsInstitute of Parasitology, Slovak Academy of Sciences, 04001Košice, SlovakiaDivision of Veterinary Epidemiology and EconomicsDimethyl sulfoxide (DMSO) is an amphipathic solvent for molecules in in vitro tests for detection of anthelmintic resistance of gastrointestinal nematodes (GIN). It has been shown to have a concentration-dependent detrimental effect on Caenorhabditis elegans, a free-living nematode. If GIN are likewise affected, using DMSO in egg-hatch test and larval development test (LDT) may confound their results. Therefore, the DMSO concentration was determined at which it exerted an inhibitory effect on GIN larval development to the third stage.https://doi.org/10.2478/jvetres-2025-0016anthelmintic resistancedmsofive-parameter logistic curveinhibitory concentrationtrichostrongylidae |
| spellingShingle | Mickiewicz Marcin Nowek Zofia Czopowicz Michał Moroz-Fik Agata Potărniche Adrian-Valentin Biernacka Kinga Szaluś-Jordanow Olga Górski Paweł Antonopoulos Alistair Markowska-Daniel Iwona Várady Marián Kaba Jarosław Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test Journal of Veterinary Research anthelmintic resistance dmso five-parameter logistic curve inhibitory concentration trichostrongylidae |
| title | Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test |
| title_full | Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test |
| title_fullStr | Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test |
| title_full_unstemmed | Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test |
| title_short | Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test |
| title_sort | inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test |
| topic | anthelmintic resistance dmso five-parameter logistic curve inhibitory concentration trichostrongylidae |
| url | https://doi.org/10.2478/jvetres-2025-0016 |
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