Compound lactic acid bacteria enhance the aerobic stability of Sesbania cannabina and corn mixed silage
Abstract Background The strategic delay of aerobic deterioration in Sesbania cannabina and corn (SC) mixed silage, coupled with effective fermentation, could increase the protein-rich silage utilization by ruminants. Thus, we sought to investigate the role of a compound lactic acid bacteria (LAB) in...
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BMC
2025-02-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-025-03781-3 |
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| author | Muhammad Tahir Tianwei Wang Jiaqi Zhang Tianqi Xia Xian Deng Xiaofeng Cao Jin Zhong |
| author_facet | Muhammad Tahir Tianwei Wang Jiaqi Zhang Tianqi Xia Xian Deng Xiaofeng Cao Jin Zhong |
| author_sort | Muhammad Tahir |
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| description | Abstract Background The strategic delay of aerobic deterioration in Sesbania cannabina and corn (SC) mixed silage, coupled with effective fermentation, could increase the protein-rich silage utilization by ruminants. Thus, we sought to investigate the role of a compound lactic acid bacteria (LAB) inoculant (Lactobacillus plantarum + Lactobacillus farciminis + Lactobacillus buchneri + Lactobacillus hilgardii; at a level of 106 CFU/g fresh weight) in enhancing the aerobic stability of SC mixed silage. Specifically, we focused on the potential for corn supplementation to improve fermentation quality while concurrently increasing the susceptibility of SC mixed silage to aerobic spoilage. Results Results revealed that compound LAB additive diversified the microbial community of SC mixed silage, making Lactobacillus hilgardii and Lactobacillus buchneri dominant bacterial species, while decreasing the abundance of Kazachstania humilis fungal specie. As a result, the LAB-treated mixed silages had higher acetic acid contents and lower yeast populations. Aerobic stability analysis revealed that the SC mixed silages with a high corn proportion deteriorated rapidly when the silages were exposed to air. The high aerobic stability of the LAB-treated mixed silages especially S7C3 contrasted with the low acetic acid concentrations in the CK mixed silages (processed with sterilized water), concomitant with increased Kazachstania humilis abundance. Conclusion Our study revealed that inoculation with a compound LAB additive altered the consequences of aerobic exposure by increasing acetic acid production after ensiling, promoting diverse bacterial populations, and mitigating the negative effects of fungi on the aerobic stability of SC mixed silage. |
| format | Article |
| id | doaj-art-8dee39a2dc344bcdbbada18ab3d4c90b |
| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj-art-8dee39a2dc344bcdbbada18ab3d4c90b2025-02-09T12:15:25ZengBMCBMC Microbiology1471-21802025-02-0125111510.1186/s12866-025-03781-3Compound lactic acid bacteria enhance the aerobic stability of Sesbania cannabina and corn mixed silageMuhammad Tahir0Tianwei Wang1Jiaqi Zhang2Tianqi Xia3Xian Deng4Xiaofeng Cao5Jin Zhong6State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesInstitute of Genetics and Developmental Biology, Chinese Academy of SciencesInstitute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesAbstract Background The strategic delay of aerobic deterioration in Sesbania cannabina and corn (SC) mixed silage, coupled with effective fermentation, could increase the protein-rich silage utilization by ruminants. Thus, we sought to investigate the role of a compound lactic acid bacteria (LAB) inoculant (Lactobacillus plantarum + Lactobacillus farciminis + Lactobacillus buchneri + Lactobacillus hilgardii; at a level of 106 CFU/g fresh weight) in enhancing the aerobic stability of SC mixed silage. Specifically, we focused on the potential for corn supplementation to improve fermentation quality while concurrently increasing the susceptibility of SC mixed silage to aerobic spoilage. Results Results revealed that compound LAB additive diversified the microbial community of SC mixed silage, making Lactobacillus hilgardii and Lactobacillus buchneri dominant bacterial species, while decreasing the abundance of Kazachstania humilis fungal specie. As a result, the LAB-treated mixed silages had higher acetic acid contents and lower yeast populations. Aerobic stability analysis revealed that the SC mixed silages with a high corn proportion deteriorated rapidly when the silages were exposed to air. The high aerobic stability of the LAB-treated mixed silages especially S7C3 contrasted with the low acetic acid concentrations in the CK mixed silages (processed with sterilized water), concomitant with increased Kazachstania humilis abundance. Conclusion Our study revealed that inoculation with a compound LAB additive altered the consequences of aerobic exposure by increasing acetic acid production after ensiling, promoting diverse bacterial populations, and mitigating the negative effects of fungi on the aerobic stability of SC mixed silage.https://doi.org/10.1186/s12866-025-03781-3Sesbania cannabina and corn mixed silageCompound lactic acid bacteria additiveSilage qualityMicrobial communityAerobic stability |
| spellingShingle | Muhammad Tahir Tianwei Wang Jiaqi Zhang Tianqi Xia Xian Deng Xiaofeng Cao Jin Zhong Compound lactic acid bacteria enhance the aerobic stability of Sesbania cannabina and corn mixed silage BMC Microbiology Sesbania cannabina and corn mixed silage Compound lactic acid bacteria additive Silage quality Microbial community Aerobic stability |
| title | Compound lactic acid bacteria enhance the aerobic stability of Sesbania cannabina and corn mixed silage |
| title_full | Compound lactic acid bacteria enhance the aerobic stability of Sesbania cannabina and corn mixed silage |
| title_fullStr | Compound lactic acid bacteria enhance the aerobic stability of Sesbania cannabina and corn mixed silage |
| title_full_unstemmed | Compound lactic acid bacteria enhance the aerobic stability of Sesbania cannabina and corn mixed silage |
| title_short | Compound lactic acid bacteria enhance the aerobic stability of Sesbania cannabina and corn mixed silage |
| title_sort | compound lactic acid bacteria enhance the aerobic stability of sesbania cannabina and corn mixed silage |
| topic | Sesbania cannabina and corn mixed silage Compound lactic acid bacteria additive Silage quality Microbial community Aerobic stability |
| url | https://doi.org/10.1186/s12866-025-03781-3 |
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