Detection of Adulterated Honey by Fluorescence Excitation-Emission Matrices
Honey is a frequent target of adulteration through inappropriate production practices and origin mislabelling. Current methods for the detection of adulterated honey are time and labor consuming, require highly skilled personnel, and lengthy sample preparation. Fluorescence spectroscopy overcomes su...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2018/8395212 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556688296640512 |
|---|---|
| author | Tatjana Dramićanin Lea Lenhardt Acković Ivana Zeković Miroslav D. Dramićanin |
| author_facet | Tatjana Dramićanin Lea Lenhardt Acković Ivana Zeković Miroslav D. Dramićanin |
| author_sort | Tatjana Dramićanin |
| collection | DOAJ |
| description | Honey is a frequent target of adulteration through inappropriate production practices and origin mislabelling. Current methods for the detection of adulterated honey are time and labor consuming, require highly skilled personnel, and lengthy sample preparation. Fluorescence spectroscopy overcomes such drawbacks, as it is fast and noncontact and requires minimal sample preparation. In this paper, the application of fluorescence spectroscopy coupled with statistical tools for the detection of adulterated honey is demonstrated. For this purpose, fluorescence excitation-emission matrices were measured for 99 samples of different types of natural honey and 15 adulterated honey samples (in 3 technical replicas for each sample). Statistical t-test showed that significant differences between fluorescence of natural and adulterated honey samples exist in 5 spectral regions: (1) excitation: 240–265 nm, emission: 370–495 nm; (2) excitation: 280–320 nm, emission: 390–470 nm; (3) excitation: 260–285 nm, emission: 320–370 nm; (4) excitation: 310–360 nm, emission: 370–470 nm; and (5) excitation: 375–435 nm, emission: 440–520 nm, in which majority of fluorescence comes from the aromatic amino acids, phenolic compounds, and fluorescent Maillard reaction products. Principal component analysis confirmed these findings and showed that 90% of variance in fluorescence is accumulated in the first two principal components, which can be used for the discrimination of fake honey samples. The classification of honey from fluorescence data is demonstrated with a linear discriminant analysis (LDA). When subjected to LDA, total fluorescence intensities of selected spectral regions provided classification of honey (natural or adulterated) with 100% accuracy. In addition, it is demonstrated that intensities of honey emissions in each of these spectral regions may serve as criteria for the discrimination between natural and fake honey. |
| format | Article |
| id | doaj-art-8de0a6e5498d4303a34b9d9cf8944aa5 |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-8de0a6e5498d4303a34b9d9cf8944aa52025-02-03T05:44:40ZengWileyJournal of Spectroscopy2314-49202314-49392018-01-01201810.1155/2018/83952128395212Detection of Adulterated Honey by Fluorescence Excitation-Emission MatricesTatjana Dramićanin0Lea Lenhardt Acković1Ivana Zeković2Miroslav D. Dramićanin3Vinča Institute of Nuclear Sciences, University of Belgrade, Belgrade 11001, SerbiaVinča Institute of Nuclear Sciences, University of Belgrade, Belgrade 11001, SerbiaVinča Institute of Nuclear Sciences, University of Belgrade, Belgrade 11001, SerbiaVinča Institute of Nuclear Sciences, University of Belgrade, Belgrade 11001, SerbiaHoney is a frequent target of adulteration through inappropriate production practices and origin mislabelling. Current methods for the detection of adulterated honey are time and labor consuming, require highly skilled personnel, and lengthy sample preparation. Fluorescence spectroscopy overcomes such drawbacks, as it is fast and noncontact and requires minimal sample preparation. In this paper, the application of fluorescence spectroscopy coupled with statistical tools for the detection of adulterated honey is demonstrated. For this purpose, fluorescence excitation-emission matrices were measured for 99 samples of different types of natural honey and 15 adulterated honey samples (in 3 technical replicas for each sample). Statistical t-test showed that significant differences between fluorescence of natural and adulterated honey samples exist in 5 spectral regions: (1) excitation: 240–265 nm, emission: 370–495 nm; (2) excitation: 280–320 nm, emission: 390–470 nm; (3) excitation: 260–285 nm, emission: 320–370 nm; (4) excitation: 310–360 nm, emission: 370–470 nm; and (5) excitation: 375–435 nm, emission: 440–520 nm, in which majority of fluorescence comes from the aromatic amino acids, phenolic compounds, and fluorescent Maillard reaction products. Principal component analysis confirmed these findings and showed that 90% of variance in fluorescence is accumulated in the first two principal components, which can be used for the discrimination of fake honey samples. The classification of honey from fluorescence data is demonstrated with a linear discriminant analysis (LDA). When subjected to LDA, total fluorescence intensities of selected spectral regions provided classification of honey (natural or adulterated) with 100% accuracy. In addition, it is demonstrated that intensities of honey emissions in each of these spectral regions may serve as criteria for the discrimination between natural and fake honey.http://dx.doi.org/10.1155/2018/8395212 |
| spellingShingle | Tatjana Dramićanin Lea Lenhardt Acković Ivana Zeković Miroslav D. Dramićanin Detection of Adulterated Honey by Fluorescence Excitation-Emission Matrices Journal of Spectroscopy |
| title | Detection of Adulterated Honey by Fluorescence Excitation-Emission Matrices |
| title_full | Detection of Adulterated Honey by Fluorescence Excitation-Emission Matrices |
| title_fullStr | Detection of Adulterated Honey by Fluorescence Excitation-Emission Matrices |
| title_full_unstemmed | Detection of Adulterated Honey by Fluorescence Excitation-Emission Matrices |
| title_short | Detection of Adulterated Honey by Fluorescence Excitation-Emission Matrices |
| title_sort | detection of adulterated honey by fluorescence excitation emission matrices |
| url | http://dx.doi.org/10.1155/2018/8395212 |
| work_keys_str_mv | AT tatjanadramicanin detectionofadulteratedhoneybyfluorescenceexcitationemissionmatrices AT lealenhardtackovic detectionofadulteratedhoneybyfluorescenceexcitationemissionmatrices AT ivanazekovic detectionofadulteratedhoneybyfluorescenceexcitationemissionmatrices AT miroslavddramicanin detectionofadulteratedhoneybyfluorescenceexcitationemissionmatrices |