Partial Characterization of Novel Bacteriocin SF1 Produced by Shigella flexneri and Their Lethal Activity on Members of Gut Microbiota
A strain of Shigella flexneri producing bacteriocin was isolated from a patient with diarrhea. The main objective of this study was to isolate and partially characterize the bacteriocin. The producing microorganism was identified using biochemical, serological, and molecular methods. The lethal acti...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2019/6747190 |
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| author | Carlos Padilla Verónica Carrasco-Sánchez Andrés Padilla Olga Lobos |
| author_facet | Carlos Padilla Verónica Carrasco-Sánchez Andrés Padilla Olga Lobos |
| author_sort | Carlos Padilla |
| collection | DOAJ |
| description | A strain of Shigella flexneri producing bacteriocin was isolated from a patient with diarrhea. The main objective of this study was to isolate and partially characterize the bacteriocin. The producing microorganism was identified using biochemical, serological, and molecular methods. The lethal activity of the S. flexneri strain was studied using the drop method. This bacterial strain showed activity against different strains of E. coli and B. fragilis. Using immunological techniques, it was determined that S. flexneri belongs to serotype 2a, and by PCR, the presence of the ipaH plasmid was determined. By chromatographic techniques, it was determined that the bacteriocin is a peptide of high purity with a molecular weight of 66294.094 Da. The amino acid composition and sequence were determined by the Edman reaction, and a sequence of 619 amino acid residues was obtained. Only in five positions of this sequence, the amino acid glutamine changed to glutamic acid with respect to colicin U produced by S. boydii. From an ecological point of view, it could be assumed that SF1 bacteriocin contributes to eliminate some members of the normal microbiota of the human intestine, facilitating colonization and then producing the invasion process that characterizes the pathogenicity of Shigella. |
| format | Article |
| id | doaj-art-8ddcb844024d4284aeb4d6ff3d62b83c |
| institution | OA Journals |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-8ddcb844024d4284aeb4d6ff3d62b83c2025-08-20T02:09:41ZengWileyInternational Journal of Microbiology1687-918X1687-91982019-01-01201910.1155/2019/67471906747190Partial Characterization of Novel Bacteriocin SF1 Produced by Shigella flexneri and Their Lethal Activity on Members of Gut MicrobiotaCarlos Padilla0Verónica Carrasco-Sánchez1Andrés Padilla2Olga Lobos3Departamento de Microbiología, Facultad de Ciencias de la Salud, Universidad de Talca, Talca 3460000, ChileDepartamento de Microbiología, Facultad de Ciencias de la Salud, Universidad de Talca, Talca 3460000, ChileServicio de Medicina Interna, Hospital Regional de Talca, Talca 3460000, ChileDepartamento de Microbiología, Facultad de Ciencias de la Salud, Universidad de Talca, Talca 3460000, ChileA strain of Shigella flexneri producing bacteriocin was isolated from a patient with diarrhea. The main objective of this study was to isolate and partially characterize the bacteriocin. The producing microorganism was identified using biochemical, serological, and molecular methods. The lethal activity of the S. flexneri strain was studied using the drop method. This bacterial strain showed activity against different strains of E. coli and B. fragilis. Using immunological techniques, it was determined that S. flexneri belongs to serotype 2a, and by PCR, the presence of the ipaH plasmid was determined. By chromatographic techniques, it was determined that the bacteriocin is a peptide of high purity with a molecular weight of 66294.094 Da. The amino acid composition and sequence were determined by the Edman reaction, and a sequence of 619 amino acid residues was obtained. Only in five positions of this sequence, the amino acid glutamine changed to glutamic acid with respect to colicin U produced by S. boydii. From an ecological point of view, it could be assumed that SF1 bacteriocin contributes to eliminate some members of the normal microbiota of the human intestine, facilitating colonization and then producing the invasion process that characterizes the pathogenicity of Shigella.http://dx.doi.org/10.1155/2019/6747190 |
| spellingShingle | Carlos Padilla Verónica Carrasco-Sánchez Andrés Padilla Olga Lobos Partial Characterization of Novel Bacteriocin SF1 Produced by Shigella flexneri and Their Lethal Activity on Members of Gut Microbiota International Journal of Microbiology |
| title | Partial Characterization of Novel Bacteriocin SF1 Produced by Shigella flexneri and Their Lethal Activity on Members of Gut Microbiota |
| title_full | Partial Characterization of Novel Bacteriocin SF1 Produced by Shigella flexneri and Their Lethal Activity on Members of Gut Microbiota |
| title_fullStr | Partial Characterization of Novel Bacteriocin SF1 Produced by Shigella flexneri and Their Lethal Activity on Members of Gut Microbiota |
| title_full_unstemmed | Partial Characterization of Novel Bacteriocin SF1 Produced by Shigella flexneri and Their Lethal Activity on Members of Gut Microbiota |
| title_short | Partial Characterization of Novel Bacteriocin SF1 Produced by Shigella flexneri and Their Lethal Activity on Members of Gut Microbiota |
| title_sort | partial characterization of novel bacteriocin sf1 produced by shigella flexneri and their lethal activity on members of gut microbiota |
| url | http://dx.doi.org/10.1155/2019/6747190 |
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