Unraveling ER dimerization dynamics in endocrine disruption based on a BRET-focused approach
Endocrine-disrupting chemicals (EDCs) are exogenous compounds that interact with the estrogen receptor (ER), thereby disrupting estrogen-mediated signaling. In a previous study, we employed a bioluminescence resonance energy transfer (BRET) system to assess ER dimerization for detecting EDCs. To fur...
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Taylor & Francis Group
2025-12-01
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| Series: | Animal Cells and Systems |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2025.2481984 |
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| author | Soomin Yum Haksoo Lee Yong-Kook Kwon Gunyoung Lee Hye-Young Lee HyeSook Youn BuHyun Youn |
| author_facet | Soomin Yum Haksoo Lee Yong-Kook Kwon Gunyoung Lee Hye-Young Lee HyeSook Youn BuHyun Youn |
| author_sort | Soomin Yum |
| collection | DOAJ |
| description | Endocrine-disrupting chemicals (EDCs) are exogenous compounds that interact with the estrogen receptor (ER), thereby disrupting estrogen-mediated signaling. In a previous study, we employed a bioluminescence resonance energy transfer (BRET) system to assess ER dimerization for detecting EDCs. To further determine whether the BRET assay could be used independently to identify EDCs, we investigated ER-EDC interactions before and after dimerization. Results from isothermal titration calorimetry (ITC) and dynamic light scattering (DLS) revealed that ER dimerization can be mediated by EDCs. Consequently, the BRET assay proved effective in detecting dimerization and clarifying its relevance to EDC-induced signaling disruption. Additionally, to examine EDC-induced transcriptional changes, we performed chromatin immunoprecipitation sequencing (ChIP-seq), followed by gene ontology (GO) analysis. These analyses demonstrated that EDCs affect various signaling pathways, including those involved in antibody-dependent cytotoxicity, bone morphogenetic protein (BMP) signaling in cardiac induction, and hepatocyte growth factor receptor signaling. Overall, this study elucidates the molecular mechanisms by which EDCs influence ER dimerization and signaling. These findings highlight the utility of the BRET-based assay for EDC detection and contribute to a deeper understanding of the systemic effects of EDCs on endocrine disruption. |
| format | Article |
| id | doaj-art-8dd23dca89fe49f0861437ef560eea12 |
| institution | DOAJ |
| issn | 1976-8354 2151-2485 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj-art-8dd23dca89fe49f0861437ef560eea122025-08-20T03:19:02ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852025-12-0129128229510.1080/19768354.2025.2481984Unraveling ER dimerization dynamics in endocrine disruption based on a BRET-focused approachSoomin Yum0Haksoo Lee1Yong-Kook Kwon2Gunyoung Lee3Hye-Young Lee4HyeSook Youn5BuHyun Youn6Department of Integrated Biological Science, Pusan National University, Kumjeong-ku, Republic of KoreaDepartment of Integrated Biological Science, Pusan National University, Kumjeong-ku, Republic of KoreaFood Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Cheongju-si, Republic of KoreaFood Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Cheongju-si, Republic of KoreaFood Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Cheongju-si, Republic of KoreaDepartment of Integrative Bioscience and Biotechnology, Sejong University, Seoul, Republic of KoreaDepartment of Integrated Biological Science, Pusan National University, Kumjeong-ku, Republic of KoreaEndocrine-disrupting chemicals (EDCs) are exogenous compounds that interact with the estrogen receptor (ER), thereby disrupting estrogen-mediated signaling. In a previous study, we employed a bioluminescence resonance energy transfer (BRET) system to assess ER dimerization for detecting EDCs. To further determine whether the BRET assay could be used independently to identify EDCs, we investigated ER-EDC interactions before and after dimerization. Results from isothermal titration calorimetry (ITC) and dynamic light scattering (DLS) revealed that ER dimerization can be mediated by EDCs. Consequently, the BRET assay proved effective in detecting dimerization and clarifying its relevance to EDC-induced signaling disruption. Additionally, to examine EDC-induced transcriptional changes, we performed chromatin immunoprecipitation sequencing (ChIP-seq), followed by gene ontology (GO) analysis. These analyses demonstrated that EDCs affect various signaling pathways, including those involved in antibody-dependent cytotoxicity, bone morphogenetic protein (BMP) signaling in cardiac induction, and hepatocyte growth factor receptor signaling. Overall, this study elucidates the molecular mechanisms by which EDCs influence ER dimerization and signaling. These findings highlight the utility of the BRET-based assay for EDC detection and contribute to a deeper understanding of the systemic effects of EDCs on endocrine disruption.https://www.tandfonline.com/doi/10.1080/19768354.2025.2481984Estrogen receptor signalingbioluminescence resonance energy transfer (BRET)endocrine-disrupting chemicals (EDCs)estrogen receptor dimerizationendocrine disruption mechanism |
| spellingShingle | Soomin Yum Haksoo Lee Yong-Kook Kwon Gunyoung Lee Hye-Young Lee HyeSook Youn BuHyun Youn Unraveling ER dimerization dynamics in endocrine disruption based on a BRET-focused approach Animal Cells and Systems Estrogen receptor signaling bioluminescence resonance energy transfer (BRET) endocrine-disrupting chemicals (EDCs) estrogen receptor dimerization endocrine disruption mechanism |
| title | Unraveling ER dimerization dynamics in endocrine disruption based on a BRET-focused approach |
| title_full | Unraveling ER dimerization dynamics in endocrine disruption based on a BRET-focused approach |
| title_fullStr | Unraveling ER dimerization dynamics in endocrine disruption based on a BRET-focused approach |
| title_full_unstemmed | Unraveling ER dimerization dynamics in endocrine disruption based on a BRET-focused approach |
| title_short | Unraveling ER dimerization dynamics in endocrine disruption based on a BRET-focused approach |
| title_sort | unraveling er dimerization dynamics in endocrine disruption based on a bret focused approach |
| topic | Estrogen receptor signaling bioluminescence resonance energy transfer (BRET) endocrine-disrupting chemicals (EDCs) estrogen receptor dimerization endocrine disruption mechanism |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2025.2481984 |
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