Gut Bacterial and Lactobacilli Communities of Weaning Piglets in Response to Mannan Oligosaccharide and Sugar Beet Pulp In vitro Fermentation
Microbiota in the gastrointestinal tract (GIT) of piglets during weaning transition can experience a sharp change which could result in growth reduction and diarrhea of weaned piglets. Dietary manipulations can play an important role in attenuating such changes caused by weaning stress. Therefore, i...
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KeAi Communications Co., Ltd.
2012-01-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1671292712607901 |
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| author | Su-qin HANG Wei-yun ZHU |
| author_facet | Su-qin HANG Wei-yun ZHU |
| author_sort | Su-qin HANG |
| collection | DOAJ |
| description | Microbiota in the gastrointestinal tract (GIT) of piglets during weaning transition can experience a sharp change which could result in growth reduction and diarrhea of weaned piglets. Dietary manipulations can play an important role in attenuating such changes caused by weaning stress. Therefore, ileal and colonic contents of weaned piglets were used as inocula, mannan oligosaccharide (MOS) or sugar beet pulp (SBP) was supplied as single energy sources to investigate effects of MOS or SBP on the shifts of gastro-intestinal microflora and lactobacilli populations. The universal bacteriaand lactobacilli-specific PCR/denaturing gradient gel electrophoresis (DGGE), cloning and sequencing techniques were used. DGGE profiles of the universal bacteria showed that great changes were found in the position, numbers and intensity of dominant bands after fermentation. The similarity of bacterial community between ileum and colon was increased to 85-97% by MOS or SBP treatment after fermentation from the similarity with 20% before fermentation. MOS treatment significantly increased the bacterial diversity and band number in both ileal and colonic fermentation (P<0.05). SBP treatment significantly increased the bacterial diversity and band number in colon (P<0.05). It implies that some species were enriched by the addition of MOS or SBP to increase the similarity and diversity of bacterial community in weaned piglets. Five specific bands appearing in MOS or SBP treatment group after fermentation were cloned and sequenced, the changes of species related to Prevotella and Ruminococcus were observed. Two bands related to uncultured bacterium with 98% similarity were detected by MOS or SBP treatment. However, there were no effects on the similarity, diversity index and lactobacilli species revealed by MOS or SBP treatment. These results imply that MOS or SBP could have beneficial effects on the weaning piglets by stablizing microbiota in the GIT microflora. |
| format | Article |
| id | doaj-art-8dcafeedd36b46e9bd3ad6985ab798ec |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-8dcafeedd36b46e9bd3ad6985ab798ec2025-08-20T03:56:55ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192012-01-0111112213310.1016/S1671-2927(12)60790-1Gut Bacterial and Lactobacilli Communities of Weaning Piglets in Response to Mannan Oligosaccharide and Sugar Beet Pulp In vitro FermentationSu-qin HANG0Wei-yun ZHU1HANG Su-qin, Mobile: 13512532889; Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaCorrespondence ZHU Wei-yun, Tel: +86-25-84395523, Fax: +86-25-84395314; Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaMicrobiota in the gastrointestinal tract (GIT) of piglets during weaning transition can experience a sharp change which could result in growth reduction and diarrhea of weaned piglets. Dietary manipulations can play an important role in attenuating such changes caused by weaning stress. Therefore, ileal and colonic contents of weaned piglets were used as inocula, mannan oligosaccharide (MOS) or sugar beet pulp (SBP) was supplied as single energy sources to investigate effects of MOS or SBP on the shifts of gastro-intestinal microflora and lactobacilli populations. The universal bacteriaand lactobacilli-specific PCR/denaturing gradient gel electrophoresis (DGGE), cloning and sequencing techniques were used. DGGE profiles of the universal bacteria showed that great changes were found in the position, numbers and intensity of dominant bands after fermentation. The similarity of bacterial community between ileum and colon was increased to 85-97% by MOS or SBP treatment after fermentation from the similarity with 20% before fermentation. MOS treatment significantly increased the bacterial diversity and band number in both ileal and colonic fermentation (P<0.05). SBP treatment significantly increased the bacterial diversity and band number in colon (P<0.05). It implies that some species were enriched by the addition of MOS or SBP to increase the similarity and diversity of bacterial community in weaned piglets. Five specific bands appearing in MOS or SBP treatment group after fermentation were cloned and sequenced, the changes of species related to Prevotella and Ruminococcus were observed. Two bands related to uncultured bacterium with 98% similarity were detected by MOS or SBP treatment. However, there were no effects on the similarity, diversity index and lactobacilli species revealed by MOS or SBP treatment. These results imply that MOS or SBP could have beneficial effects on the weaning piglets by stablizing microbiota in the GIT microflora.http://www.sciencedirect.com/science/article/pii/S1671292712607901mannan oligosaccharide (MOS)sugar beet pulp (SBP)bacterial communitieslactobacilliweaning piglets |
| spellingShingle | Su-qin HANG Wei-yun ZHU Gut Bacterial and Lactobacilli Communities of Weaning Piglets in Response to Mannan Oligosaccharide and Sugar Beet Pulp In vitro Fermentation Journal of Integrative Agriculture mannan oligosaccharide (MOS) sugar beet pulp (SBP) bacterial communities lactobacilli weaning piglets |
| title | Gut Bacterial and Lactobacilli Communities of Weaning Piglets in Response to Mannan Oligosaccharide and Sugar Beet Pulp In vitro Fermentation |
| title_full | Gut Bacterial and Lactobacilli Communities of Weaning Piglets in Response to Mannan Oligosaccharide and Sugar Beet Pulp In vitro Fermentation |
| title_fullStr | Gut Bacterial and Lactobacilli Communities of Weaning Piglets in Response to Mannan Oligosaccharide and Sugar Beet Pulp In vitro Fermentation |
| title_full_unstemmed | Gut Bacterial and Lactobacilli Communities of Weaning Piglets in Response to Mannan Oligosaccharide and Sugar Beet Pulp In vitro Fermentation |
| title_short | Gut Bacterial and Lactobacilli Communities of Weaning Piglets in Response to Mannan Oligosaccharide and Sugar Beet Pulp In vitro Fermentation |
| title_sort | gut bacterial and lactobacilli communities of weaning piglets in response to mannan oligosaccharide and sugar beet pulp in vitro fermentation |
| topic | mannan oligosaccharide (MOS) sugar beet pulp (SBP) bacterial communities lactobacilli weaning piglets |
| url | http://www.sciencedirect.com/science/article/pii/S1671292712607901 |
| work_keys_str_mv | AT suqinhang gutbacterialandlactobacillicommunitiesofweaningpigletsinresponsetomannanoligosaccharideandsugarbeetpulpinvitrofermentation AT weiyunzhu gutbacterialandlactobacillicommunitiesofweaningpigletsinresponsetomannanoligosaccharideandsugarbeetpulpinvitrofermentation |