The role of the aging process and related factor EMP1 in promoting progression of resectable pancreatic cancer
Pancreatic cancer (PC) is a highly malignant neoplasm of the digestive system. The primary objective of this investigation is to elucidate the intricate mechanisms underlying the role of the aging process and the related factor Epithelial membrane protein 1 (EMP1) in PC progression. We established a...
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KeAi Communications Co., Ltd.
2025-09-01
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| Series: | Genes and Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352304224002873 |
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| author | Junfeng Zhang Jianyou Gu Tao Zhang Renpei Xia Jianbo Li Mingda Tan Yongjun Yang Jifeng Xiang Bin Xie Rong Tang Wangge Li Xianxing Wang Shixiang Guo Huaizhi Wang |
| author_facet | Junfeng Zhang Jianyou Gu Tao Zhang Renpei Xia Jianbo Li Mingda Tan Yongjun Yang Jifeng Xiang Bin Xie Rong Tang Wangge Li Xianxing Wang Shixiang Guo Huaizhi Wang |
| author_sort | Junfeng Zhang |
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| description | Pancreatic cancer (PC) is a highly malignant neoplasm of the digestive system. The primary objective of this investigation is to elucidate the intricate mechanisms underlying the role of the aging process and the related factor Epithelial membrane protein 1 (EMP1) in PC progression. We established a prognostic model pertinent to the aging process that could be applied in postoperative PC patients. In vitro assays were employed to elucidate the impact of EMP1 on PC cell function. We employed lentiviral vectors for both knockdown and overexpression of EMP1 in Panc02 cells, followed by the establishment of subcutaneous, pulmonary metastasis, and orthotopic pancreatic liver metastasis models in mice. Using tissue microarrays, we evaluated the expression of EMP1 and its downstream entities, and then conducted clinical correlation analysis. A predictive Age-Related Score (ARS) system based on age-associated prognostic genes was developed, offering precise prognostic predictions for postoperative PC patients, which could be applied well at the single-cell level, showing diverse aging, epithelial–mesenchymal transition (EMT), cell migration, cell proliferation, and PI3K/AKT signaling activity in high and low ARS risk cells. EMP1 was identified as a pivotal molecule in the ARS system and is associated with poor prognosis. Besides, EMP1 could enhance the proliferation, migration, and invasion of PC cells both in vitro and in vivo by augmenting the PI3K/AKT signaling cascade. In essence, this research formulated an aging-centric prognostic model for postoperative PC and pinpointed EMP1 as an oncogenic factor facilitating tumor cell EMT during the aging trajectory in resectable PC patients. |
| format | Article |
| id | doaj-art-8dc0c0a4f92046f0afd828a52dd0fc88 |
| institution | Kabale University |
| issn | 2352-3042 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Genes and Diseases |
| spelling | doaj-art-8dc0c0a4f92046f0afd828a52dd0fc882025-08-20T03:29:15ZengKeAi Communications Co., Ltd.Genes and Diseases2352-30422025-09-0112510149010.1016/j.gendis.2024.101490The role of the aging process and related factor EMP1 in promoting progression of resectable pancreatic cancerJunfeng Zhang0Jianyou Gu1Tao Zhang2Renpei Xia3Jianbo Li4Mingda Tan5Yongjun Yang6Jifeng Xiang7Bin Xie8Rong Tang9Wangge Li10Xianxing Wang11Shixiang Guo12Huaizhi Wang13University of Chinese Academy of Sciences (UCAS) Chongqing School, Chongqing Medical University, Chongqing 401147, China; Institute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China; Chongqing Key Laboratory of Intelligent Medicine Engineering for Hepatopancreatobiliary Diseases, Chongqing 401147, ChinaInstitute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China; Chongqing Key Laboratory of Intelligent Medicine Engineering for Hepatopancreatobiliary Diseases, Chongqing 401147, ChinaUniversity of Chinese Academy of Sciences (UCAS) Chongqing School, Chongqing Medical University, Chongqing 401147, China; Institute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China; Chongqing Key Laboratory of Intelligent Medicine Engineering for Hepatopancreatobiliary Diseases, Chongqing 401147, ChinaInstitute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China; Chongqing Key Laboratory of Intelligent Medicine Engineering for Hepatopancreatobiliary Diseases, Chongqing 401147, ChinaUniversity of Chinese Academy of Sciences (UCAS) Chongqing School, Chongqing Medical University, Chongqing 401147, China; Institute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China; Chongqing Key Laboratory of Intelligent Medicine Engineering for Hepatopancreatobiliary Diseases, Chongqing 401147, ChinaUniversity of Chinese Academy of Sciences (UCAS) Chongqing School, Chongqing Medical University, Chongqing 401147, China; Institute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China; Chongqing Key Laboratory of Intelligent Medicine Engineering for Hepatopancreatobiliary Diseases, Chongqing 401147, ChinaUniversity of Chinese Academy of Sciences (UCAS) Chongqing School, Chongqing Medical University, Chongqing 401147, China; Institute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China; Chongqing Key Laboratory of Intelligent Medicine Engineering for Hepatopancreatobiliary Diseases, Chongqing 401147, ChinaInstitute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China; Chongqing Key Laboratory of Intelligent Medicine Engineering for Hepatopancreatobiliary Diseases, Chongqing 401147, ChinaUniversity of Chinese Academy of Sciences (UCAS) Chongqing School, Chongqing Medical University, Chongqing 401147, China; Institute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China; Chongqing Key Laboratory of Intelligent Medicine Engineering for Hepatopancreatobiliary Diseases, Chongqing 401147, ChinaInstitute of Clinical Nutrition, Chongqing General Hospital, Chongqing University, Chongqing 401147, ChinaDepartment of Clinical Laboratory, The First Affilitated Hospital of Chongqing Medical University, Chongqing 401147, ChinaInstitute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China; Chongqing Key Laboratory of Intelligent Medicine Engineering for Hepatopancreatobiliary Diseases, Chongqing 401147, China; Corresponding author. Institute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China.Institute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China; Chongqing Key Laboratory of Intelligent Medicine Engineering for Hepatopancreatobiliary Diseases, Chongqing 401147, China; Chongqing School, University of Chinese Academy of Sciences, Chongqing 400714, China; Corresponding author. Institute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China.University of Chinese Academy of Sciences (UCAS) Chongqing School, Chongqing Medical University, Chongqing 401147, China; Institute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing University, Chongqing 401147, China; Chongqing Key Laboratory of Intelligent Medicine Engineering for Hepatopancreatobiliary Diseases, Chongqing 401147, China; Corresponding author. University of Chinese Academy of Sciences (UCAS) Chongqing School, Chongqing Medical University, Chongqing 401147, China.Pancreatic cancer (PC) is a highly malignant neoplasm of the digestive system. The primary objective of this investigation is to elucidate the intricate mechanisms underlying the role of the aging process and the related factor Epithelial membrane protein 1 (EMP1) in PC progression. We established a prognostic model pertinent to the aging process that could be applied in postoperative PC patients. In vitro assays were employed to elucidate the impact of EMP1 on PC cell function. We employed lentiviral vectors for both knockdown and overexpression of EMP1 in Panc02 cells, followed by the establishment of subcutaneous, pulmonary metastasis, and orthotopic pancreatic liver metastasis models in mice. Using tissue microarrays, we evaluated the expression of EMP1 and its downstream entities, and then conducted clinical correlation analysis. A predictive Age-Related Score (ARS) system based on age-associated prognostic genes was developed, offering precise prognostic predictions for postoperative PC patients, which could be applied well at the single-cell level, showing diverse aging, epithelial–mesenchymal transition (EMT), cell migration, cell proliferation, and PI3K/AKT signaling activity in high and low ARS risk cells. EMP1 was identified as a pivotal molecule in the ARS system and is associated with poor prognosis. Besides, EMP1 could enhance the proliferation, migration, and invasion of PC cells both in vitro and in vivo by augmenting the PI3K/AKT signaling cascade. In essence, this research formulated an aging-centric prognostic model for postoperative PC and pinpointed EMP1 as an oncogenic factor facilitating tumor cell EMT during the aging trajectory in resectable PC patients.http://www.sciencedirect.com/science/article/pii/S2352304224002873AgingEMP1Pancreatic cancerPI3K/AKTPostoperative prognosis |
| spellingShingle | Junfeng Zhang Jianyou Gu Tao Zhang Renpei Xia Jianbo Li Mingda Tan Yongjun Yang Jifeng Xiang Bin Xie Rong Tang Wangge Li Xianxing Wang Shixiang Guo Huaizhi Wang The role of the aging process and related factor EMP1 in promoting progression of resectable pancreatic cancer Genes and Diseases Aging EMP1 Pancreatic cancer PI3K/AKT Postoperative prognosis |
| title | The role of the aging process and related factor EMP1 in promoting progression of resectable pancreatic cancer |
| title_full | The role of the aging process and related factor EMP1 in promoting progression of resectable pancreatic cancer |
| title_fullStr | The role of the aging process and related factor EMP1 in promoting progression of resectable pancreatic cancer |
| title_full_unstemmed | The role of the aging process and related factor EMP1 in promoting progression of resectable pancreatic cancer |
| title_short | The role of the aging process and related factor EMP1 in promoting progression of resectable pancreatic cancer |
| title_sort | role of the aging process and related factor emp1 in promoting progression of resectable pancreatic cancer |
| topic | Aging EMP1 Pancreatic cancer PI3K/AKT Postoperative prognosis |
| url | http://www.sciencedirect.com/science/article/pii/S2352304224002873 |
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