Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo
Mammalian inner ear harbors diverse cell types that are essential for hearing and balance. Adenovirus is one of the major vectors to deliver genes into the inner ear for functional studies and hair cell regeneration. To identify adenovirus vectors that target specific cell subtypes in the inner ear,...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/9409846 |
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| author | Yilai Shu Yong Tao Wenyan Li Jun Shen Zhengmin Wang Zheng-Yi Chen |
| author_facet | Yilai Shu Yong Tao Wenyan Li Jun Shen Zhengmin Wang Zheng-Yi Chen |
| author_sort | Yilai Shu |
| collection | DOAJ |
| description | Mammalian inner ear harbors diverse cell types that are essential for hearing and balance. Adenovirus is one of the major vectors to deliver genes into the inner ear for functional studies and hair cell regeneration. To identify adenovirus vectors that target specific cell subtypes in the inner ear, we studied three adenovirus vectors, carrying a reporter gene encoding green fluorescent protein (GFP) from two vendors or with a genome editing gene Cre recombinase (Cre), by injection into postnatal days 0 (P0) and 4 (P4) mouse cochlea through scala media by cochleostomy in vivo. We found three adenovirus vectors transduced mouse inner ear cells with different specificities and expression levels, depending on the type of adenoviral vectors and the age of mice. The most frequently targeted region was the cochlear sensory epithelium, including auditory hair cells and supporting cells. Adenovirus with GFP transduced utricular supporting cells as well. This study shows that adenovirus vectors are capable of efficiently and specifically transducing different cell types in the mammalian inner ear and provides useful tools to study inner ear gene function and to evaluate gene therapy to treat hearing loss and vestibular dysfunction. |
| format | Article |
| id | doaj-art-8dbe8ba187124a339f87ad57a0822c85 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-8dbe8ba187124a339f87ad57a0822c852025-02-03T01:10:00ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/94098469409846Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In VivoYilai Shu0Yong Tao1Wenyan Li2Jun Shen3Zhengmin Wang4Zheng-Yi Chen5Department of Otolaryngology, Harvard Medical School and Eaton-Peabody Laboratories, Massachusetts Eye & Ear Infirmary, Boston, MA 02114, USADepartment of Otolaryngology, Harvard Medical School and Eaton-Peabody Laboratories, Massachusetts Eye & Ear Infirmary, Boston, MA 02114, USADepartment of Otolaryngology, Harvard Medical School and Eaton-Peabody Laboratories, Massachusetts Eye & Ear Infirmary, Boston, MA 02114, USADepartment of Pathology, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USADepartment of Otolaryngology-Head and Neck Surgery, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, ChinaDepartment of Otolaryngology, Harvard Medical School and Eaton-Peabody Laboratories, Massachusetts Eye & Ear Infirmary, Boston, MA 02114, USAMammalian inner ear harbors diverse cell types that are essential for hearing and balance. Adenovirus is one of the major vectors to deliver genes into the inner ear for functional studies and hair cell regeneration. To identify adenovirus vectors that target specific cell subtypes in the inner ear, we studied three adenovirus vectors, carrying a reporter gene encoding green fluorescent protein (GFP) from two vendors or with a genome editing gene Cre recombinase (Cre), by injection into postnatal days 0 (P0) and 4 (P4) mouse cochlea through scala media by cochleostomy in vivo. We found three adenovirus vectors transduced mouse inner ear cells with different specificities and expression levels, depending on the type of adenoviral vectors and the age of mice. The most frequently targeted region was the cochlear sensory epithelium, including auditory hair cells and supporting cells. Adenovirus with GFP transduced utricular supporting cells as well. This study shows that adenovirus vectors are capable of efficiently and specifically transducing different cell types in the mammalian inner ear and provides useful tools to study inner ear gene function and to evaluate gene therapy to treat hearing loss and vestibular dysfunction.http://dx.doi.org/10.1155/2016/9409846 |
| spellingShingle | Yilai Shu Yong Tao Wenyan Li Jun Shen Zhengmin Wang Zheng-Yi Chen Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo Neural Plasticity |
| title | Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo |
| title_full | Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo |
| title_fullStr | Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo |
| title_full_unstemmed | Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo |
| title_short | Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo |
| title_sort | adenovirus vectors target several cell subtypes of mammalian inner ear in vivo |
| url | http://dx.doi.org/10.1155/2016/9409846 |
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