A method for siRNA-mediated knockdown of target genes in RA-induced neurogenesis using P19 cells
This study presents a comprehensive protocol for siRNA-mediated knockdown in the differentiation of P19 cells into neuronal-like cells. Utilizing a retinoic acid (RA)-induced neurogenesis model, P19 cells were cultured under specific conditions that facilitated the formation of embryoid bodies (EBs)...
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Main Authors: | Hossein Khodadadi, Hiroaki Taniguchi |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2025-06-01
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Series: | MethodsX |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016125000251 |
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