A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases
Abstract Tick-borne infections are the most common vector-borne diseases in the USA. Ticks harbor and transmit several infections with Lyme disease being the most common tickborne infection in the US and Europe. Lack of awareness about tick populations, specific diagnostic tests, and overlapping sig...
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2025-01-01
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| author | Hari Krishnan Krishnamurthy Vasanth Jayaraman Karthik Krishna Tianhao Wang Kang Bei Chithra Changalath Shiny Matilda Alex J. Rai Renata Welc-Falęciak Agnieszka Pawełczyk Lucas S. Blanton Aleš Chrdle Andrea Fořtová Daniel Růžek Gheyath K. Nasrallah Laith J. Abu-Raddadi Duaa W. Al-Sadeq Marah Abed Alhakim Abdallah Daniele Lilleri Chiara Fornara Piera D’Angelo Milena Furione Maria Söderlund-Venermo Klaus Hedman Dimosthenis Chochlakis Eirini Makridaki Artemis Ntoula Anna Psaroulaki Angélica Escárcega-Ávila John J. Rajasekaran |
| author_facet | Hari Krishnan Krishnamurthy Vasanth Jayaraman Karthik Krishna Tianhao Wang Kang Bei Chithra Changalath Shiny Matilda Alex J. Rai Renata Welc-Falęciak Agnieszka Pawełczyk Lucas S. Blanton Aleš Chrdle Andrea Fořtová Daniel Růžek Gheyath K. Nasrallah Laith J. Abu-Raddadi Duaa W. Al-Sadeq Marah Abed Alhakim Abdallah Daniele Lilleri Chiara Fornara Piera D’Angelo Milena Furione Maria Söderlund-Venermo Klaus Hedman Dimosthenis Chochlakis Eirini Makridaki Artemis Ntoula Anna Psaroulaki Angélica Escárcega-Ávila John J. Rajasekaran |
| author_sort | Hari Krishnan Krishnamurthy |
| collection | DOAJ |
| description | Abstract Tick-borne infections are the most common vector-borne diseases in the USA. Ticks harbor and transmit several infections with Lyme disease being the most common tickborne infection in the US and Europe. Lack of awareness about tick populations, specific diagnostic tests, and overlapping signs and symptoms of tick-borne infections can often lead to misdiagnosis affecting treatment and the prevalence data reported especially for non-Lyme tick-borne infections. The diagnostic tests currently available for tick-borne diseases are severely limited in their ability to provide accurate results and cannot detect multiple pathogens in a single run. The multiplex protein microarray developed at Vibrant was designed to detect multiple serological antibodies thereby detecting exposure to multiple pathogens simultaneously. Our microarray in its present form can accommodate 400 antigens (molecules that can bind to specific antibodies) and can multiplex across antigen types, whole cell lysates, recombinant proteins, and peptides. A designed array containing multiple antigens of several microbes including Borrelia burgdorferi, the Lyme disease spirochete, was manufactured and evaluated. The immunoglobulin M (IgM) and G (IgG) responses against several tick-borne microbes and other infectious agents were analyzed for analytical and clinical performance. The microarray improved IgM and IgG sensitivities and specificities of individual microbes when compared with the respective gold standards. The testing was also performed in a single run in comparison to multiple runs needed for comparable testing standards. In summary, our study presents a flexible multiplex microarray platform that can provide quick results with high sensitivity and specificity for evaluating exposure to varied infectious agents especially tick-borne pathogens. |
| format | Article |
| id | doaj-art-8dad5886a76a44c39dfba750e0b85f2a |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-8dad5886a76a44c39dfba750e0b85f2a2025-01-26T12:25:29ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-024-84467-0A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseasesHari Krishnan Krishnamurthy0Vasanth Jayaraman1Karthik Krishna2Tianhao Wang3Kang Bei4Chithra Changalath5Shiny Matilda6Alex J. Rai7Renata Welc-Falęciak8Agnieszka Pawełczyk9Lucas S. Blanton10Aleš Chrdle11Andrea Fořtová12Daniel Růžek13Gheyath K. Nasrallah14Laith J. Abu-Raddadi15Duaa W. Al-Sadeq16Marah Abed Alhakim Abdallah17Daniele Lilleri18Chiara Fornara19Piera D’Angelo20Milena Furione21Maria Söderlund-Venermo22Klaus Hedman23Dimosthenis Chochlakis24Eirini Makridaki25Artemis Ntoula26Anna Psaroulaki27Angélica Escárcega-Ávila28John J. Rajasekaran29Vibrant Sciences LLC.Vibrant Sciences LLC.Vibrant Sciences LLC.Vibrant Sciences LLC.Vibrant Sciences LLC.Vibrant America LLC.Vibrant America LLC.Irving Medical Center, Department of Pathology & Cell Biology, Columbia UniversityDepartment of Parasitology, Faculty of Biology, Diagnostic Laboratory of Parasitic Diseases and Zoonotic Infections, Biological and Chemical Research Centre, University of WarsawDepartment of Immunopathology of Infectious and Parasitic Diseases, Medical University of WarsawDivision of Infectious Diseases, Department of Internal Medicine, University of Texas Medical BranchDepartment of Infectious Diseases, Hospital Ceske BudejoviceVeterinary Research InstituteInstitute of Parasitology, Biology Centre of the Czech Academy of SciencesBiomedical Sciences Department, College of Health Sciences, Qatar UniversityBiomedical Sciences Department, College of Health Sciences, Qatar UniversityBiomedical Sciences Department, College of Health Sciences, Qatar UniversityBiomedical Sciences Department, College of Health Sciences, Qatar UniversityMicrobiologia E Virologia, Fondazione IRCCS Policlinico San MatteoMicrobiologia E Virologia, Fondazione IRCCS Policlinico San MatteoMicrobiologia E Virologia, Fondazione IRCCS Policlinico San MatteoMicrobiologia E Virologia, Fondazione IRCCS Policlinico San MatteoUniversity of HelsinkiUniversity of HelsinkiLaboratory of Clinical Microbiology and Microbial Pathogenesis, School of Medicine, University of CreteLaboratory of Clinical Microbiology and Microbial Pathogenesis, School of Medicine, University of CreteLaboratory of Clinical Microbiology and Microbial Pathogenesis, School of Medicine, University of CreteLaboratory of Clinical Microbiology and Microbial Pathogenesis, School of Medicine, University of CreteLaboratory of Biotechnology at, Institute of Biomedical Science, University of Ciudad JuárezVibrant Sciences LLC.Abstract Tick-borne infections are the most common vector-borne diseases in the USA. Ticks harbor and transmit several infections with Lyme disease being the most common tickborne infection in the US and Europe. Lack of awareness about tick populations, specific diagnostic tests, and overlapping signs and symptoms of tick-borne infections can often lead to misdiagnosis affecting treatment and the prevalence data reported especially for non-Lyme tick-borne infections. The diagnostic tests currently available for tick-borne diseases are severely limited in their ability to provide accurate results and cannot detect multiple pathogens in a single run. The multiplex protein microarray developed at Vibrant was designed to detect multiple serological antibodies thereby detecting exposure to multiple pathogens simultaneously. Our microarray in its present form can accommodate 400 antigens (molecules that can bind to specific antibodies) and can multiplex across antigen types, whole cell lysates, recombinant proteins, and peptides. A designed array containing multiple antigens of several microbes including Borrelia burgdorferi, the Lyme disease spirochete, was manufactured and evaluated. The immunoglobulin M (IgM) and G (IgG) responses against several tick-borne microbes and other infectious agents were analyzed for analytical and clinical performance. The microarray improved IgM and IgG sensitivities and specificities of individual microbes when compared with the respective gold standards. The testing was also performed in a single run in comparison to multiple runs needed for comparable testing standards. In summary, our study presents a flexible multiplex microarray platform that can provide quick results with high sensitivity and specificity for evaluating exposure to varied infectious agents especially tick-borne pathogens.https://doi.org/10.1038/s41598-024-84467-0Tick-borne infectionsLyme diseaseMultiplexMicroarrayImmunoglobulinTick bite |
| spellingShingle | Hari Krishnan Krishnamurthy Vasanth Jayaraman Karthik Krishna Tianhao Wang Kang Bei Chithra Changalath Shiny Matilda Alex J. Rai Renata Welc-Falęciak Agnieszka Pawełczyk Lucas S. Blanton Aleš Chrdle Andrea Fořtová Daniel Růžek Gheyath K. Nasrallah Laith J. Abu-Raddadi Duaa W. Al-Sadeq Marah Abed Alhakim Abdallah Daniele Lilleri Chiara Fornara Piera D’Angelo Milena Furione Maria Söderlund-Venermo Klaus Hedman Dimosthenis Chochlakis Eirini Makridaki Artemis Ntoula Anna Psaroulaki Angélica Escárcega-Ávila John J. Rajasekaran A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases Scientific Reports Tick-borne infections Lyme disease Multiplex Microarray Immunoglobulin Tick bite |
| title | A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases |
| title_full | A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases |
| title_fullStr | A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases |
| title_full_unstemmed | A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases |
| title_short | A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases |
| title_sort | customizable multiplex protein microarray for antibody testing and its application for tick borne and other infectious diseases |
| topic | Tick-borne infections Lyme disease Multiplex Microarray Immunoglobulin Tick bite |
| url | https://doi.org/10.1038/s41598-024-84467-0 |
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