Mindin promotes the production of reactive oxygen species to regulate intestinal microbiota in kuruma shrimp Marsupenaeus japonicus
The Mindin/Spondin family, as host extracellular matrix components, comprises key immune-related molecules in both mammals and Drosophila. While the detailed mechanisms of Mindin's function and its role in initiating an effective immune response have been elucidated in model animals, its immune...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | Comparative Immunology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950311625000138 |
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| author | Kai-Min Zhou Bao-Rui Zhao Jie Gao Yongzhen Zhao Yueling Zhang Xian-Wei Wang |
| author_facet | Kai-Min Zhou Bao-Rui Zhao Jie Gao Yongzhen Zhao Yueling Zhang Xian-Wei Wang |
| author_sort | Kai-Min Zhou |
| collection | DOAJ |
| description | The Mindin/Spondin family, as host extracellular matrix components, comprises key immune-related molecules in both mammals and Drosophila. While the detailed mechanisms of Mindin's function and its role in initiating an effective immune response have been elucidated in model animals, its immune regulatory function in invertebrates remains largely unknown. In the present study, we identified and characterized a Mindin homolog from kuruma shrimp (Marsupenaeus japonicus), which was significantly up-regulated upon Vibrio anguillarum infection. Our findings revealed that Mindin plays a crucial protective role in the antibacterial response by regulating dual oxidase 2 (Duox2) expression. Mechanistically, Mindin recognizes V. anguillarum through its Tsp domain and simultaneously interacts with cell membrane integrin via its SpN domain. This dual interaction triggers the phosphorylation of extracellular regulated kinase (Erk), leading to the activation and phosphorylation of transcription factor Atf2. The phosphorylated Atf2 translocates to the nucleus where it induces the expression of Duox2, resulting in the production of reactive oxygen species (ROS) that effectively inhibit bacterial growth. By establishing the Mindin/Integrin/Erk/Atf2/Duox2 signaling axis, this study unveils a novel antibacterial mechanism in shrimp and provides new insights into the functional role of Mindin in invertebrate immunity. |
| format | Article |
| id | doaj-art-8d9c2e9c72b34a3d8260f235162a9dc1 |
| institution | OA Journals |
| issn | 2950-3116 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Comparative Immunology Reports |
| spelling | doaj-art-8d9c2e9c72b34a3d8260f235162a9dc12025-08-20T02:31:12ZengElsevierComparative Immunology Reports2950-31162025-06-01820020710.1016/j.cirep.2025.200207Mindin promotes the production of reactive oxygen species to regulate intestinal microbiota in kuruma shrimp Marsupenaeus japonicusKai-Min Zhou0Bao-Rui Zhao1Jie Gao2Yongzhen Zhao3Yueling Zhang4Xian-Wei Wang5Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, Shandong 266237, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, Shandong 266237, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, Shandong 266237, ChinaGuangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning 530021, ChinaGuangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou 515063, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, Shandong 266237, China; Corresponding author at: School of Life Sciences, Shandong University, Qingdao, Shandong 266237, China.The Mindin/Spondin family, as host extracellular matrix components, comprises key immune-related molecules in both mammals and Drosophila. While the detailed mechanisms of Mindin's function and its role in initiating an effective immune response have been elucidated in model animals, its immune regulatory function in invertebrates remains largely unknown. In the present study, we identified and characterized a Mindin homolog from kuruma shrimp (Marsupenaeus japonicus), which was significantly up-regulated upon Vibrio anguillarum infection. Our findings revealed that Mindin plays a crucial protective role in the antibacterial response by regulating dual oxidase 2 (Duox2) expression. Mechanistically, Mindin recognizes V. anguillarum through its Tsp domain and simultaneously interacts with cell membrane integrin via its SpN domain. This dual interaction triggers the phosphorylation of extracellular regulated kinase (Erk), leading to the activation and phosphorylation of transcription factor Atf2. The phosphorylated Atf2 translocates to the nucleus where it induces the expression of Duox2, resulting in the production of reactive oxygen species (ROS) that effectively inhibit bacterial growth. By establishing the Mindin/Integrin/Erk/Atf2/Duox2 signaling axis, this study unveils a novel antibacterial mechanism in shrimp and provides new insights into the functional role of Mindin in invertebrate immunity.http://www.sciencedirect.com/science/article/pii/S2950311625000138Innate immunityInvertebrateMindinReactive oxygen species |
| spellingShingle | Kai-Min Zhou Bao-Rui Zhao Jie Gao Yongzhen Zhao Yueling Zhang Xian-Wei Wang Mindin promotes the production of reactive oxygen species to regulate intestinal microbiota in kuruma shrimp Marsupenaeus japonicus Comparative Immunology Reports Innate immunity Invertebrate Mindin Reactive oxygen species |
| title | Mindin promotes the production of reactive oxygen species to regulate intestinal microbiota in kuruma shrimp Marsupenaeus japonicus |
| title_full | Mindin promotes the production of reactive oxygen species to regulate intestinal microbiota in kuruma shrimp Marsupenaeus japonicus |
| title_fullStr | Mindin promotes the production of reactive oxygen species to regulate intestinal microbiota in kuruma shrimp Marsupenaeus japonicus |
| title_full_unstemmed | Mindin promotes the production of reactive oxygen species to regulate intestinal microbiota in kuruma shrimp Marsupenaeus japonicus |
| title_short | Mindin promotes the production of reactive oxygen species to regulate intestinal microbiota in kuruma shrimp Marsupenaeus japonicus |
| title_sort | mindin promotes the production of reactive oxygen species to regulate intestinal microbiota in kuruma shrimp marsupenaeus japonicus |
| topic | Innate immunity Invertebrate Mindin Reactive oxygen species |
| url | http://www.sciencedirect.com/science/article/pii/S2950311625000138 |
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