Genome-wide identification and characterization of MdCYP86A family in apple
The cytochrome P450 enzyme is one of the largest protein families identified in cuticle biosynthesis, plant growth, development, and stress response. In the present study, 28 members of the MdCYP86A family were identified in apple (Malus × domestica Borkh.). Chromosomal localization analysis found t...
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2024-01-01
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| Series: | Fruit Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/frures-0024-0024 |
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| author | Hui-Min Lv Rui-Han Qi Zi-Han Yu Yao-Yang Man Yan-Hui Lv Han Jiang Tao Wang Ya-Li Zhang Yuan-Yuan Li |
| author_facet | Hui-Min Lv Rui-Han Qi Zi-Han Yu Yao-Yang Man Yan-Hui Lv Han Jiang Tao Wang Ya-Li Zhang Yuan-Yuan Li |
| author_sort | Hui-Min Lv |
| collection | DOAJ |
| description | The cytochrome P450 enzyme is one of the largest protein families identified in cuticle biosynthesis, plant growth, development, and stress response. In the present study, 28 members of the MdCYP86A family were identified in apple (Malus × domestica Borkh.). Chromosomal localization analysis found that 28 genes were distributed on 13 chromosomes in apple. Phylogenetic and conserved motifs analysis showed that MdCYP86As in apple is evolutionarily most closely related to Arabidopsis thaliana. Each gene contains a cytochrome P450 domain, suggesting that MdCYP86As are highly conserved during the evolution of the species. Cis-acting element analysis of the promoter identified various elements associated with stress response. MdCYP86A21 was cloned in the apple genome to investigate the function of MdCYP86A family genes. MdCYP86A21 responded to abiotic stress, including PEG, NaCl, and ABA. The tissue-specific expression patterns revealed that the highest expression tissue was in flower by real-time quantitative reverse transcription (RT-qPCR). Subcellular localization showed that MdCYP86A21 was localized in the endoplasmic reticulum. Further, the function of MdCYP86A21 to enhance the resistance to PEG and NaCl stress in apple calli was verified. These results contribute to further study on the function of MdCYP86As and provide a molecular basis for the study of apple stress tolerance. |
| format | Article |
| id | doaj-art-8d8bf574e4f34badb36bd35def49b850 |
| institution | OA Journals |
| issn | 2769-4615 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Fruit Research |
| spelling | doaj-art-8d8bf574e4f34badb36bd35def49b8502025-08-20T02:27:11ZengMaximum Academic PressFruit Research2769-46152024-01-014111110.48130/frures-0024-0024frures-0024-0024Genome-wide identification and characterization of MdCYP86A family in appleHui-Min Lv0Rui-Han Qi1Zi-Han Yu2Yao-Yang Man3Yan-Hui Lv4Han Jiang5Tao Wang6Ya-Li Zhang7Yuan-Yuan Li8National Apple Engineering Technology Research Center, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, ChinaNational Apple Engineering Technology Research Center, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, ChinaNational Apple Engineering Technology Research Center, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, ChinaNational Apple Engineering Technology Research Center, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, ChinaNational Apple Engineering Technology Research Center, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, ChinaNational Apple Engineering Technology Research Center, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, ChinaTai' an Institute For Food And Drug Control, Tai-An 271000, Shandong, ChinaNational Apple Engineering Technology Research Center, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, ChinaNational Apple Engineering Technology Research Center, Shandong Collaborative Innovation, Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, ChinaThe cytochrome P450 enzyme is one of the largest protein families identified in cuticle biosynthesis, plant growth, development, and stress response. In the present study, 28 members of the MdCYP86A family were identified in apple (Malus × domestica Borkh.). Chromosomal localization analysis found that 28 genes were distributed on 13 chromosomes in apple. Phylogenetic and conserved motifs analysis showed that MdCYP86As in apple is evolutionarily most closely related to Arabidopsis thaliana. Each gene contains a cytochrome P450 domain, suggesting that MdCYP86As are highly conserved during the evolution of the species. Cis-acting element analysis of the promoter identified various elements associated with stress response. MdCYP86A21 was cloned in the apple genome to investigate the function of MdCYP86A family genes. MdCYP86A21 responded to abiotic stress, including PEG, NaCl, and ABA. The tissue-specific expression patterns revealed that the highest expression tissue was in flower by real-time quantitative reverse transcription (RT-qPCR). Subcellular localization showed that MdCYP86A21 was localized in the endoplasmic reticulum. Further, the function of MdCYP86A21 to enhance the resistance to PEG and NaCl stress in apple calli was verified. These results contribute to further study on the function of MdCYP86As and provide a molecular basis for the study of apple stress tolerance.https://www.maxapress.com/article/doi/10.48130/frures-0024-0024applemdcyp86a gene familymdcyp86a21abiotic stress |
| spellingShingle | Hui-Min Lv Rui-Han Qi Zi-Han Yu Yao-Yang Man Yan-Hui Lv Han Jiang Tao Wang Ya-Li Zhang Yuan-Yuan Li Genome-wide identification and characterization of MdCYP86A family in apple Fruit Research apple mdcyp86a gene family mdcyp86a21 abiotic stress |
| title | Genome-wide identification and characterization of MdCYP86A family in apple |
| title_full | Genome-wide identification and characterization of MdCYP86A family in apple |
| title_fullStr | Genome-wide identification and characterization of MdCYP86A family in apple |
| title_full_unstemmed | Genome-wide identification and characterization of MdCYP86A family in apple |
| title_short | Genome-wide identification and characterization of MdCYP86A family in apple |
| title_sort | genome wide identification and characterization of mdcyp86a family in apple |
| topic | apple mdcyp86a gene family mdcyp86a21 abiotic stress |
| url | https://www.maxapress.com/article/doi/10.48130/frures-0024-0024 |
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