Cytokine Levels and Human Herpesviruses in Saliva from Clinical Periodontal Healthy Subjects with Peri-Implantitis: A Case-Control Study
This study evaluated the presence of cytokines (IL-1β, IL-2, IL-4, IL-6, MCP-1, MIP-1α, MIP-1β, and TNF-α) and human herpesvirus (HSV1, HSV2, EBV, CMV, VZV, HHV6, HHV7, and HHV8) in saliva samples taken from subjects with and without peri-implantitis. Forty-two periodontally healthy subjects were di...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/6020625 |
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| author | Jaime S. Marques Filho Jorge Gobara Gustavo Vargas da Silva Salomao Laura M. Sumita Jamil A. Shibli Renato G. Viana Humberto O. Schwartz Filho Claudio Sergio Pannuti Paulo Henrique Braz-Silva Debora Pallos |
| author_facet | Jaime S. Marques Filho Jorge Gobara Gustavo Vargas da Silva Salomao Laura M. Sumita Jamil A. Shibli Renato G. Viana Humberto O. Schwartz Filho Claudio Sergio Pannuti Paulo Henrique Braz-Silva Debora Pallos |
| author_sort | Jaime S. Marques Filho |
| collection | DOAJ |
| description | This study evaluated the presence of cytokines (IL-1β, IL-2, IL-4, IL-6, MCP-1, MIP-1α, MIP-1β, and TNF-α) and human herpesvirus (HSV1, HSV2, EBV, CMV, VZV, HHV6, HHV7, and HHV8) in saliva samples taken from subjects with and without peri-implantitis. Forty-two periodontally healthy subjects were divided according to peri-implant condition: healthy and peri-implantitis groups. The clinical parameters as probing depth, clinical attachment level, plaque index, gingival bleeding, bleeding on probing, and suppuration were evaluated. For cytokine detection, multiplex analysis was performed, and PCR assay was used to identify herpesviruses. No significant differences were found in cytokine levels between groups (p>0.05). The presence of herpesvirus was 1.97-fold higher in patients with peri-implantitis (odds ratio, CI 0.52–7.49). The association of the presence or absence of herpesvirus with the salivary markers was statistically significant for MIP-1β (p=0.0087) and TNF-α (p=0.0437) only in the peri-implantitis group. The presence of herpesviruses in patients with peri-implantitis suggests the development of a proinflammatory environment, which is characterized by increased expression of MIP-1β and TNF-α in saliva. |
| format | Article |
| id | doaj-art-8d7aca952ca24e37a67d07c17a44e6ea |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-8d7aca952ca24e37a67d07c17a44e6ea2025-08-20T03:37:56ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/60206256020625Cytokine Levels and Human Herpesviruses in Saliva from Clinical Periodontal Healthy Subjects with Peri-Implantitis: A Case-Control StudyJaime S. Marques Filho0Jorge Gobara1Gustavo Vargas da Silva Salomao2Laura M. Sumita3Jamil A. Shibli4Renato G. Viana5Humberto O. Schwartz Filho6Claudio Sergio Pannuti7Paulo Henrique Braz-Silva8Debora Pallos9Department of Dentistry, University Santo Amaro, São Paulo, SP, BrazilDepartment of Dentistry, University Santo Amaro, São Paulo, SP, BrazilDivision of General Pathology, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, SP, BrazilDepartment of Virology, Institute of Tropical Medicine of São Paulo, University of São Paulo, São Paulo, SP, BrazilDepartment of Periodontology and Oral Implantology, University of Guarulhos, Guarulhos, SP, BrazilDepartment of Periodontology and Oral Implantology, University of Guarulhos, Guarulhos, SP, BrazilDepartment of Dentistry, University Santo Amaro, São Paulo, SP, BrazilDepartment of Virology, Institute of Tropical Medicine of São Paulo, University of São Paulo, São Paulo, SP, BrazilDivision of General Pathology, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, SP, BrazilDepartment of Dentistry, University Santo Amaro, São Paulo, SP, BrazilThis study evaluated the presence of cytokines (IL-1β, IL-2, IL-4, IL-6, MCP-1, MIP-1α, MIP-1β, and TNF-α) and human herpesvirus (HSV1, HSV2, EBV, CMV, VZV, HHV6, HHV7, and HHV8) in saliva samples taken from subjects with and without peri-implantitis. Forty-two periodontally healthy subjects were divided according to peri-implant condition: healthy and peri-implantitis groups. The clinical parameters as probing depth, clinical attachment level, plaque index, gingival bleeding, bleeding on probing, and suppuration were evaluated. For cytokine detection, multiplex analysis was performed, and PCR assay was used to identify herpesviruses. No significant differences were found in cytokine levels between groups (p>0.05). The presence of herpesvirus was 1.97-fold higher in patients with peri-implantitis (odds ratio, CI 0.52–7.49). The association of the presence or absence of herpesvirus with the salivary markers was statistically significant for MIP-1β (p=0.0087) and TNF-α (p=0.0437) only in the peri-implantitis group. The presence of herpesviruses in patients with peri-implantitis suggests the development of a proinflammatory environment, which is characterized by increased expression of MIP-1β and TNF-α in saliva.http://dx.doi.org/10.1155/2018/6020625 |
| spellingShingle | Jaime S. Marques Filho Jorge Gobara Gustavo Vargas da Silva Salomao Laura M. Sumita Jamil A. Shibli Renato G. Viana Humberto O. Schwartz Filho Claudio Sergio Pannuti Paulo Henrique Braz-Silva Debora Pallos Cytokine Levels and Human Herpesviruses in Saliva from Clinical Periodontal Healthy Subjects with Peri-Implantitis: A Case-Control Study Mediators of Inflammation |
| title | Cytokine Levels and Human Herpesviruses in Saliva from Clinical Periodontal Healthy Subjects with Peri-Implantitis: A Case-Control Study |
| title_full | Cytokine Levels and Human Herpesviruses in Saliva from Clinical Periodontal Healthy Subjects with Peri-Implantitis: A Case-Control Study |
| title_fullStr | Cytokine Levels and Human Herpesviruses in Saliva from Clinical Periodontal Healthy Subjects with Peri-Implantitis: A Case-Control Study |
| title_full_unstemmed | Cytokine Levels and Human Herpesviruses in Saliva from Clinical Periodontal Healthy Subjects with Peri-Implantitis: A Case-Control Study |
| title_short | Cytokine Levels and Human Herpesviruses in Saliva from Clinical Periodontal Healthy Subjects with Peri-Implantitis: A Case-Control Study |
| title_sort | cytokine levels and human herpesviruses in saliva from clinical periodontal healthy subjects with peri implantitis a case control study |
| url | http://dx.doi.org/10.1155/2018/6020625 |
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