Association between Lyn tyrosine kinase and renal injury in patients with systemic lupus erythematosus

Background To investigate protein kinase Lyn expression in patients with systemic lupus erythematosus (SLE) or lupus nephritis (LN) and its association with clinical markers.Methods Sixty-one inpatients with SLE were categorized into low SLE Disease Activity Index (SLEDAI) group (SLEDAI < 6, n = ...

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Main Authors: Jian Sun, Yihong Gu, Wei Lin, Linjuan Luo, Wei Li, Jinrong He, Zishun Zhan, Juan Tang
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Renal Failure
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Online Access:https://www.tandfonline.com/doi/10.1080/0886022X.2025.2515532
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author Jian Sun
Yihong Gu
Wei Lin
Linjuan Luo
Wei Li
Jinrong He
Zishun Zhan
Juan Tang
author_facet Jian Sun
Yihong Gu
Wei Lin
Linjuan Luo
Wei Li
Jinrong He
Zishun Zhan
Juan Tang
author_sort Jian Sun
collection DOAJ
description Background To investigate protein kinase Lyn expression in patients with systemic lupus erythematosus (SLE) or lupus nephritis (LN) and its association with clinical markers.Methods Sixty-one inpatients with SLE were categorized into low SLE Disease Activity Index (SLEDAI) group (SLEDAI < 6, n = 32) and high SLEDAI group (SLEDAI ≥ 6, n = 29). Thirty volunteers served as healthy controls (HC). SLE patients were further divided into non-LN (n = 31) and LN (n = 30) groups. Lyn mRNA and protein levels in peripheral blood mononuclear cells (PBMCs) were analyzed and correlations between Lyn expression and SLEDAI, clinical parameters were examined. Renal biopsies from LN (n = 24) were collected for immunohistochemical analysis of Lyn level.Results Compared to the HC group, SLE patients exhibited a decline in both Lyn mRNA and protein expression in PBMCs, with significant downregulation in the high SLEDAI group versus the low SLEDAI group and a notable reduction in the LN group relative to the non-LN group. Lyn levels negatively correlated with SLEDAI and 24-hour urine protein (24h-U-pro) while positively correlated with serum C3, C4, hemoglobin, and albumin. Multiple linear regression indicated an independent association between Lyn mRNA and SLEDAI. ROC curve analysis suggested Lyn as a reliable predictor for SLE, LN, and lupus activity, with histopathological examination revealing that decreased Lyn expression correlated negatively with the renal pathology index (r = −0.8016, p = 0.0016).Conclusions The protein kinase Lyn was inversely associated with disease activity, albuminuria level and renal pathology activity in SLE and may be a promising biomarker for assessing LN disease activity.
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spelling doaj-art-8d7a57960fb64c8aa6948bef6c5241202025-08-20T03:44:52ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492025-12-0147110.1080/0886022X.2025.2515532Association between Lyn tyrosine kinase and renal injury in patients with systemic lupus erythematosusJian Sun0Yihong Gu1Wei Lin2Linjuan Luo3Wei Li4Jinrong He5Zishun Zhan6Juan Tang7Department of Nephrology and Rheumatology, Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Nephrology and Rheumatology, Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Pathology, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Nephrology and Rheumatology, Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Nephrology and Rheumatology, Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Nephrology and Rheumatology, Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Nephrology and Rheumatology, Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Nephrology and Rheumatology, Third Xiangya Hospital, Central South University, Changsha, ChinaBackground To investigate protein kinase Lyn expression in patients with systemic lupus erythematosus (SLE) or lupus nephritis (LN) and its association with clinical markers.Methods Sixty-one inpatients with SLE were categorized into low SLE Disease Activity Index (SLEDAI) group (SLEDAI < 6, n = 32) and high SLEDAI group (SLEDAI ≥ 6, n = 29). Thirty volunteers served as healthy controls (HC). SLE patients were further divided into non-LN (n = 31) and LN (n = 30) groups. Lyn mRNA and protein levels in peripheral blood mononuclear cells (PBMCs) were analyzed and correlations between Lyn expression and SLEDAI, clinical parameters were examined. Renal biopsies from LN (n = 24) were collected for immunohistochemical analysis of Lyn level.Results Compared to the HC group, SLE patients exhibited a decline in both Lyn mRNA and protein expression in PBMCs, with significant downregulation in the high SLEDAI group versus the low SLEDAI group and a notable reduction in the LN group relative to the non-LN group. Lyn levels negatively correlated with SLEDAI and 24-hour urine protein (24h-U-pro) while positively correlated with serum C3, C4, hemoglobin, and albumin. Multiple linear regression indicated an independent association between Lyn mRNA and SLEDAI. ROC curve analysis suggested Lyn as a reliable predictor for SLE, LN, and lupus activity, with histopathological examination revealing that decreased Lyn expression correlated negatively with the renal pathology index (r = −0.8016, p = 0.0016).Conclusions The protein kinase Lyn was inversely associated with disease activity, albuminuria level and renal pathology activity in SLE and may be a promising biomarker for assessing LN disease activity.https://www.tandfonline.com/doi/10.1080/0886022X.2025.2515532Protein kinase Lynsystemic lupus erythematosuslupus nephritisSLEDAIrenal pathology index
spellingShingle Jian Sun
Yihong Gu
Wei Lin
Linjuan Luo
Wei Li
Jinrong He
Zishun Zhan
Juan Tang
Association between Lyn tyrosine kinase and renal injury in patients with systemic lupus erythematosus
Renal Failure
Protein kinase Lyn
systemic lupus erythematosus
lupus nephritis
SLEDAI
renal pathology index
title Association between Lyn tyrosine kinase and renal injury in patients with systemic lupus erythematosus
title_full Association between Lyn tyrosine kinase and renal injury in patients with systemic lupus erythematosus
title_fullStr Association between Lyn tyrosine kinase and renal injury in patients with systemic lupus erythematosus
title_full_unstemmed Association between Lyn tyrosine kinase and renal injury in patients with systemic lupus erythematosus
title_short Association between Lyn tyrosine kinase and renal injury in patients with systemic lupus erythematosus
title_sort association between lyn tyrosine kinase and renal injury in patients with systemic lupus erythematosus
topic Protein kinase Lyn
systemic lupus erythematosus
lupus nephritis
SLEDAI
renal pathology index
url https://www.tandfonline.com/doi/10.1080/0886022X.2025.2515532
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