Protocol for RNA interference in gene expression in adult insects of Triatoma infestans (Hemiptera: Reduviidae)
Summary: RNA interference (RNAi) is a technique that enables post-transcriptional gene silencing in organisms. Here, we present a protocol for silencing the clock gene period (per) in adult insects of Triatoma infestans using RNAi. We describe steps for insect rearing, synthesis of double-stranded R...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002710 |
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| author | Lourdes E. Córdoba Alicia R. Pérez de Rosas Beatriz A. García María M. Stroppa |
| author_facet | Lourdes E. Córdoba Alicia R. Pérez de Rosas Beatriz A. García María M. Stroppa |
| author_sort | Lourdes E. Córdoba |
| collection | DOAJ |
| description | Summary: RNA interference (RNAi) is a technique that enables post-transcriptional gene silencing in organisms. Here, we present a protocol for silencing the clock gene period (per) in adult insects of Triatoma infestans using RNAi. We describe steps for insect rearing, synthesis of double-stranded RNA (dsRNA) of target genes, and abdominal microinjection with dsRNA. We then detail procedures for collecting and extracting RNA from heads at different time points and evaluating silencing efficiency using quantitative reverse-transcription PCR (RT-qPCR).For complete details on the use and execution of this protocol, please refer to Córdoba et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-8d7239db6e2448049c8d968f3535fbff |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-8d7239db6e2448049c8d968f3535fbff2025-08-20T03:55:22ZengElsevierSTAR Protocols2666-16672025-06-016210386510.1016/j.xpro.2025.103865Protocol for RNA interference in gene expression in adult insects of Triatoma infestans (Hemiptera: Reduviidae)Lourdes E. Córdoba0Alicia R. Pérez de Rosas1Beatriz A. García2María M. Stroppa3Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina; Corresponding authorInstituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, ArgentinaInstituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, ArgentinaInstituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina; Corresponding authorSummary: RNA interference (RNAi) is a technique that enables post-transcriptional gene silencing in organisms. Here, we present a protocol for silencing the clock gene period (per) in adult insects of Triatoma infestans using RNAi. We describe steps for insect rearing, synthesis of double-stranded RNA (dsRNA) of target genes, and abdominal microinjection with dsRNA. We then detail procedures for collecting and extracting RNA from heads at different time points and evaluating silencing efficiency using quantitative reverse-transcription PCR (RT-qPCR).For complete details on the use and execution of this protocol, please refer to Córdoba et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725002710Model OrganismsMolecular BiologySpecial IssueProtocols in Entomology |
| spellingShingle | Lourdes E. Córdoba Alicia R. Pérez de Rosas Beatriz A. García María M. Stroppa Protocol for RNA interference in gene expression in adult insects of Triatoma infestans (Hemiptera: Reduviidae) STAR Protocols Model Organisms Molecular Biology Special Issue Protocols in Entomology |
| title | Protocol for RNA interference in gene expression in adult insects of Triatoma infestans (Hemiptera: Reduviidae) |
| title_full | Protocol for RNA interference in gene expression in adult insects of Triatoma infestans (Hemiptera: Reduviidae) |
| title_fullStr | Protocol for RNA interference in gene expression in adult insects of Triatoma infestans (Hemiptera: Reduviidae) |
| title_full_unstemmed | Protocol for RNA interference in gene expression in adult insects of Triatoma infestans (Hemiptera: Reduviidae) |
| title_short | Protocol for RNA interference in gene expression in adult insects of Triatoma infestans (Hemiptera: Reduviidae) |
| title_sort | protocol for rna interference in gene expression in adult insects of triatoma infestans hemiptera reduviidae |
| topic | Model Organisms Molecular Biology Special Issue Protocols in Entomology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725002710 |
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