Molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from a university hospital in Brazil
Introduction: The emergence of Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-Kpn) isolates is attracting significant attention in nosocomial infection settings. K. pneumoniae is the main pathogen that harbours blaKPC genes. Methodology: This study evaluated 54 K. pneumon...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2017-06-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/8614 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850036922695548928 |
|---|---|
| author | Ana Carolina Polano Vivan Juliana Ferraz Rosa Camila Fonseca Rizek Marsileni Pelisson Silvia Figueiredo Costa Mariangela Hungria Renata Kobayashi Eliana Carolina Vespero |
| author_facet | Ana Carolina Polano Vivan Juliana Ferraz Rosa Camila Fonseca Rizek Marsileni Pelisson Silvia Figueiredo Costa Mariangela Hungria Renata Kobayashi Eliana Carolina Vespero |
| author_sort | Ana Carolina Polano Vivan |
| collection | DOAJ |
| description |
Introduction: The emergence of Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-Kpn) isolates is attracting significant attention in nosocomial infection settings. K. pneumoniae is the main pathogen that harbours blaKPC genes.
Methodology: This study evaluated 54 K. pneumoniae carbapenem-resistant isolates from patients hospitalized at the University Hospital of Londrina, between July 2009 and July 2010. The isolates were phenotypically screened for carbapenemase production and submitted for genotypic confirmation by polymerase chain reaction (PCR) for KPC, metallo-β-lactamases, OXA-48, and extended-spectrum beta-lactamase genes. The absence of outer membrane proteins (OMP) was investigated by SDS-PAGE. The susceptibility profile was determined by broth microdilution, according to Clinical and Laboratory Standards Institute protocol.
Results: All isolates were phenotypically positive for class A carbapenemase production, but negative for metallo-β-lactamase activity. PCR analysis demonstrated that all isolates carried blaKPC genes and sequencing showed that all strains belonged to KPC-2 subtype. Four strains did not show porin expression, and all isolates were resistant to ertapenem, meropenem, and imipenem. Susceptibility rates reached 35.2% for gentamicin, 85.2% for polymixyn B, 87% for colistin, and 98.1% for both tigecycline and fosfomycin. Pulsed-field gel electrophoresis showed six clones, and three of them predominated among the isolates.
Conclusions: KPC-2-producing K. pneumoniae is becoming predominant among carbapenem-resistant K. pneumoniae isolates at the hospital. The association of the enzyme KPC with other resistance determinants, such as loss of porins, may increase the severity of the situation of nosocomial infections. There is an urgent need to develop strategies for infection control and prevention.
|
| format | Article |
| id | doaj-art-8d717739be5742fbbd824ca9e609434f |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-8d717739be5742fbbd824ca9e609434f2025-08-20T02:57:01ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-06-01110510.3855/jidc.8614Molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from a university hospital in BrazilAna Carolina Polano Vivan0Juliana Ferraz Rosa1Camila Fonseca Rizek2Marsileni Pelisson3Silvia Figueiredo Costa4Mariangela Hungria5Renata Kobayashi6Eliana Carolina Vespero7University of the Sacred Heart, Bauru, BrazilSão Paulo University, São Paulo, BrazilSão Paulo University, São Paulo, BrazilLondrina State University, Londrina, BrazilSão Paulo University, São Paulo, BrazilEmbrapa Soja, Londrina, PR, BrazilLondrina State University, Londrina, BrazilLondrina State University, Londrina, Brazil Introduction: The emergence of Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-Kpn) isolates is attracting significant attention in nosocomial infection settings. K. pneumoniae is the main pathogen that harbours blaKPC genes. Methodology: This study evaluated 54 K. pneumoniae carbapenem-resistant isolates from patients hospitalized at the University Hospital of Londrina, between July 2009 and July 2010. The isolates were phenotypically screened for carbapenemase production and submitted for genotypic confirmation by polymerase chain reaction (PCR) for KPC, metallo-β-lactamases, OXA-48, and extended-spectrum beta-lactamase genes. The absence of outer membrane proteins (OMP) was investigated by SDS-PAGE. The susceptibility profile was determined by broth microdilution, according to Clinical and Laboratory Standards Institute protocol. Results: All isolates were phenotypically positive for class A carbapenemase production, but negative for metallo-β-lactamase activity. PCR analysis demonstrated that all isolates carried blaKPC genes and sequencing showed that all strains belonged to KPC-2 subtype. Four strains did not show porin expression, and all isolates were resistant to ertapenem, meropenem, and imipenem. Susceptibility rates reached 35.2% for gentamicin, 85.2% for polymixyn B, 87% for colistin, and 98.1% for both tigecycline and fosfomycin. Pulsed-field gel electrophoresis showed six clones, and three of them predominated among the isolates. Conclusions: KPC-2-producing K. pneumoniae is becoming predominant among carbapenem-resistant K. pneumoniae isolates at the hospital. The association of the enzyme KPC with other resistance determinants, such as loss of porins, may increase the severity of the situation of nosocomial infections. There is an urgent need to develop strategies for infection control and prevention. https://jidc.org/index.php/journal/article/view/8614Klebsiella pneumoniaecarbapenemaseKPCcarbapenem resistance |
| spellingShingle | Ana Carolina Polano Vivan Juliana Ferraz Rosa Camila Fonseca Rizek Marsileni Pelisson Silvia Figueiredo Costa Mariangela Hungria Renata Kobayashi Eliana Carolina Vespero Molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from a university hospital in Brazil Journal of Infection in Developing Countries Klebsiella pneumoniae carbapenemase KPC carbapenem resistance |
| title | Molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from a university hospital in Brazil |
| title_full | Molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from a university hospital in Brazil |
| title_fullStr | Molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from a university hospital in Brazil |
| title_full_unstemmed | Molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from a university hospital in Brazil |
| title_short | Molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from a university hospital in Brazil |
| title_sort | molecular characterization of carbapenem resistant klebsiella pneumoniae isolates from a university hospital in brazil |
| topic | Klebsiella pneumoniae carbapenemase KPC carbapenem resistance |
| url | https://jidc.org/index.php/journal/article/view/8614 |
| work_keys_str_mv | AT anacarolinapolanovivan molecularcharacterizationofcarbapenemresistantklebsiellapneumoniaeisolatesfromauniversityhospitalinbrazil AT julianaferrazrosa molecularcharacterizationofcarbapenemresistantklebsiellapneumoniaeisolatesfromauniversityhospitalinbrazil AT camilafonsecarizek molecularcharacterizationofcarbapenemresistantklebsiellapneumoniaeisolatesfromauniversityhospitalinbrazil AT marsilenipelisson molecularcharacterizationofcarbapenemresistantklebsiellapneumoniaeisolatesfromauniversityhospitalinbrazil AT silviafigueiredocosta molecularcharacterizationofcarbapenemresistantklebsiellapneumoniaeisolatesfromauniversityhospitalinbrazil AT mariangelahungria molecularcharacterizationofcarbapenemresistantklebsiellapneumoniaeisolatesfromauniversityhospitalinbrazil AT renatakobayashi molecularcharacterizationofcarbapenemresistantklebsiellapneumoniaeisolatesfromauniversityhospitalinbrazil AT elianacarolinavespero molecularcharacterizationofcarbapenemresistantklebsiellapneumoniaeisolatesfromauniversityhospitalinbrazil |